朊蛋白MAb QYQRES IgG1同种型

Recongnizes a conserved epitope (QYQRES) on the ruminant prion protein in tissues from sheep, cattle, mule deer, elk and white-tailed deer. IgG1 isotype.Useful in detection agents of transmissible spongiform encephalopathies (TSEs), including sheep scrapie, bovine spongiform encephalopathy (BSE), chronic wasting disease (CWD). Techniques include immunoassays of fresh and formalin-fixed tissues, including Western immunoblot (performs better than F89/160.1.5), immunohistochemistry, ELISA, and fluorescence flow cytometry.Lot F990803 was evaluated by immunohistochemistry (IHC) of brain and lymph node from scrapie-infected sheep, and brain and lymph node from a sheep with no known exposure to scrapie. The antibody was diluted to 3-5µg/ml and incubated for 30 minutes with pretreated tissues. Detection was performed on a Ventana immunostainer using biotinylated goat anti-mouse Ig, Sterptavidin-horseradish peroxidase and AEC as the chromogen/substrate. There was no stining of the tissue from the sheep with no exposure to was intense. The quality of this lot of antibody is excellent. Tissues for IHC with this antibody are paraffin-embedded formalin-fixed tissues, often decontaminated by incubation of formalin-fixed tissue in formic Acid for 60 minutes before re-equilibration in formalin and routine processing and embedding. Three to 5 micron sections are rehydrated, pretreated first with acid (98% formic acid 5-20 minutes, then rinsed and neutralized with several changes of 0.1 M Tris buffer, pH 7.5) then with heat (autoclaved at 121°C for 20 minutes in modified citrate buffer, pH 6.1, preferably DAKO Target Petrieval Buffer). Note: This monoclonal antibody is produced as cell culture supernatant, clarified by centrifugation, and filtered though a 0.2 micron filter. The concentration is 1.0 mg/ml in phosphate-buffered saline containing 4 mg/ml BSA preserved with 0.09% sodium azide.