- 93 次围观
产品图片
产品别名
货号/SKU
EVU301
货号/规格
1 vial
库存与交货期
1-2周
人民币价格
13285
试剂海关审批
使用人负责A/B风险申请资质
国外采购
支持/部分需签MTA
厂牌
Kerafast, Inc.
品牌
产品基础信息
From the laboratory of James E. Crowe, Jr., MD, Vanderbilt University.
产品描述信息
Product Type: | Cell Line |
Name: | EL-4-B5 |
Cell Type: | Mouse thymoma continuous cell line |
Organism: | Mouse |
Accession ID: | CVCL_5I39 |
Biosafety Level: | 2 |
Growth Conditions: | RPMI-1640, 10% FBS heat inactivated, with 2 mM glutamine, usually antibiotics (typically pen/strep or gentamicin plus amphotericin B), 10 mM HEPES, 2- mercaptoethanol (0.056 mM) |
Subculturing: | Cells are both adherent and suspension. The suspension cells are the more healthy ones. Can tolerate large splits after line is established (volume ratios of 1:10, for example). Helper function is optimal when cells are in log phase growth. Keep density below 106cells per mL at all times |
Cryopreservation: | Standard conditions (typically 10% DMSO, 90% FBS) |
Mycoplasma Tested: | Yes |
Storage: | Liquid nitrogen |
Shipped: | Dry ice |
产品安全信息
Zubler RH,Erard F,Lees RK,VanLaer M,Mingari C,Moretta L,MacDonald HR. Mutant EL-4 thymoma cells polyclonally activate murine and human B cells via direct cell interaction. J Immunol 1985, 134:3662-3668. Li W,Hanvanich M,Werner-Favre C,Brouwers N,Perrin LH,Zubler RH. Limiting dilution assay for human B cells based on their activation by mutant EL-4 thymoma cells: total and anti-malaria responder B cell frequencies. Eur J Immunol 1987, 17:887-892. Lundgren M,Persson U,Larsson P,Magnusson C,Smith CIE,Hammarstrom L, Severinson E. Interleukin 4 induces synthesis of IgE and IgG4 in human B cells. Eur J Immunol 1989, 19 :1311-1315. Kwekkeboom J, De Boer M, Tager JM, De Groot C. CD40 plays an essential role inthe activation of human B cells by murine EL4B5 cells. Immunology. 1993Jul;79(3):439-44. Steenbakkers PG, Van Wezenbeek PM, van Zanten J, The TH. Efficient generation of human anti-cytomegalovirus IgG monoclonal antibodies from preselectedantigen-specific B cells. Hum Antibodies Hybridomas. 1993 Oct;4(4):166-73. Brinkmann V, Heusser CH. T cell-dependent differentiation of human B cellsinto IgM, IgG, IgA, or IgE plasma cells: high rate of antibody production by IgE plasma cells, but limited clonal expansion of IgE precursors. Cell Immunol. 1993 Dec;152(2):323-32. Matthes T,Werner-Favre C, Tang H, Zhang X, Kindler V, Zubler RH. Cytokine mRNA expression during an in vitro response of human B lymphocytes: Kinetics of tumor necrosis factor-a, interleukin (IL) 6, IL-10 and transforming growth factor b-1 mRNAs. J Exp Med 1993, 178:521-528. DeWildt RMT,Steenbakkers PG,Pennings AHM,vandenHoogen FHJ,van Venrooij WJ, Hoet RMA. A new method for the analysis and production of monoclonal antibody fragments originating from single human B cells. J Immunol Methods 1997, 207:61-67 Leyendeckers H, Odendahl M, Lohndorf A, Irsch J, Spangfort M, Miltenyi S, Hunzelmann N, Assenmacher M, Radbruch A, Schmitz J. Corelation analysis between frequencies of circulating antigen-specific IgG-bearing memory B cells and serum titers of antigen-specific IgG. Eur J Immunol 1999, 29:1406-1417. Weitkamp J-H, Kallewaard N, Kusuhara K, Feiglstock D, Feng N, Greenberg HB, Crowe JE Jr. Generation of recombinant human monoclonal antibodies to rotavirus from single antigen-specific B cells selected with fluorescent virus-like particles. J Immunol Methods 2003, 275:223-237. If you publish research with this product, please let us know so we can cite your paper.
主要内容
细胞系EL-4-B5是鼠EL4胸腺瘤的亚旋钮,其是溴 - 脱氧尿苷突变体,并且可以用作进料层,以产生B细胞培养物,得到人单克隆抗体。il-2生产EL-4小鼠胸腺瘤细胞接受诱变与乙基甲烷 - 磺酸盐,导致T细胞杂交瘤的融合伴侣。然后发现胸苷激酶缺陷型和Oubainee抗性克隆EL-4洗衣型6.1也通过细胞接触激活小鼠和人B细胞。亚克隆产生强烈激活B细胞的EL-4-B5细胞。用B细胞生长EL4-B5以通过直接细胞接触激活B细胞以诱导抗体的增殖,分化和分泌。 EL-4-B5细胞系提供了一种从用荧光VLP选择的单一抗原特异性B细胞克隆产生重组人mAb的方法,可用于产生人体mAb,以向许多其他病毒产生蛋白质可以自组装成VLP的其他病毒从Vanderbilt University的James E. Crowe,Jr.,Jr.。
厂牌介绍
关于Kerafast Inc.
Kerafast 是一家位于波士顿的试剂公司,其主要使命是为QuanQiu科学界提供易于使用的独特实验室研究工具。我们的产品组合包括细胞系、抗体、小分子、染料等,其中许多在其他地方无法获得。自 2011 年成立以来,来自全球 190 多个机构的研究人员通过我们的在线平台提供了他们的创新试剂,无需通过传统的材料转让协议流程即可快速获取材料。
我们处理提供实验室的所有销售和运输物流,并从每次销售中返还丰厚的特许权使用费。因此,我们帮助提供实验室节省时间和资源,同时为进一步研究提供额外资金。采购科学家可以更轻松地发现和获取其他地方通常无法获得的独特试剂,同时还可以资助其他研究人员的工作。这创建了一个QuanQiu科学家社区,他们贡献和获取Reagent for the Greater Good,以加速他们自己的研究以及整体科学进步。
2018 年,Kerafast 与Absolute Antibody合并,后者是一家总部位于英国的公司,其愿景是为所有研究人员提供重组抗体技术。此次合并将两家公司聚集在一起,共同致力于改善科学界可用的研究工具的选择。
品牌标识
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