spycatcher003蛋白质

产品图片
产品英文名称
货号/SKU
EOX003
货号/规格
0.5mg
库存与交货期
1-2周
人民币价格
6700
人民币价格说明
本商品人民币2024年销售价格正在调整中,请等待更新完毕。

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试剂海关审批
使用人负责A/B风险申请资质
国外采购
支持/部分需签MTA
厂牌
Kerafast, Inc.
品牌
产品基础信息
From the laboratory of Mark Howarth, PhD, University of Oxford.
产品描述信息

 

Product Type: Protein
Name: SpyTag/SpyCatcher Protein Coupling Reagents
Accession ID: JQ478411.1 (SpyCatcher)
Source: Recombinant expression in E. coli
Molecular Weight: 15,597.7 Da (SpyCatcher003) 15,613.7 Da (SpyCatcher003, S49C); 44,867.78 Da (SpyTag003-MBP)
Amino Acid Sequence:

SpyCatcher003: SYYHHHHHHDYDIPTTENLYFQGAMVTTLSGLSGEQGPSGDMTTEEDSATHIKF

SKRDEDGRELAGATMELRDSSGKTISTWISDGHVKDFYLYPGKYTFVETAAPDGYEVATPIEFTVN

EDGQVTVDGEATEGDAHTGSSGS
SpyCatcher003 (S49C): SYYHHHHHHDYDIPTTENLYFQGAMVTTLSGLSGEQGPSGDMTTEE

DSATHIKFSKRDEDGRELAGATMELRDCSGKTISTWISDGHVKDFYLYPGKYTFVETAAPDGYE

VATPIEFTVNEDGQVTVDGEATEGDAHTGSSGS
SpyTag003: GSSHHHHHHSSGLVPRGSRGVPHIVMVDAYKRYKGSGESGKIEEGKLVIWINGD

KGYNGLAEVGKKFEKDTGIKVTVEHPDKLEEKFPQVAATGDGPDIIFWAHDRFGGYAQSGLLA

EITPDKAFQDKLYPFTWDAVRYNGKLIAYPIAVEALSLIYNKDLLPNPPKTWEEIPALDKELKA

KGKSALMFNLQEPYFTWPLIAADGGYAFKYENGKYDIKDVGVDNAGAKAGLTFLVDLIKNK

HMNADTDYSIAEAAFNKGETAMTINGPWAWSNIDTSKVNYGVTVLPTFKGQPSKPFVGVL

SAGINAASPNKELAKEFLENYLLTDEGLEAVNKDKPLGAVALKSYEEELAKDPRIAATM

ENAQKGEIMPNIPQMSAFWYAVRTAVINAASGRQTVDEALKDAQTNSSS

Fusion Tag(s): N-terminal 6xHis tag for each
Purity: >95%. Purified from Ni-NTA affinity chromatography and then by size exclusion chromatography.
Buffer: Phosphate buffered saline (PBS) pH 7.4
Concentration: 2.32mg/mL (SpyCatcher003), 2.38mg/mL (SpyCatcher003, S49C), 2.47mg/mL (SpyTag003-MBP)
Amount: 0.5mg
Storage: -80C, avoid multiple freeze-thaw cycles
Shipped: Dry ice
产品安全信息
Keeble AH, Turkki P, Stokes S, Khairil Anuar INA, Rahikainen R, Hytönen VP, Howarth M. Approaching infinite affinity through engineering of peptide-protein interaction. Proc Natl Acad Sci U S A. 2019 Dec 10;116(52):26523-26533.Zakeri B, Fierer JO, Celik E, Chittock EC, Schwarz-Linek U, Moy VT, Howarth M. Peptide tag forming a rapid covalent bond to a protein, through engineering a bacterial adhesin. Proc Natl Acad Sci U S A. 2012 Mar 20;109(12):E690-7. Veggiani G., Zakeri B., Howarth M. Superglue from Bacteria: Unbreakable Bridges for Protein Nanotechnology. Trends in Biotechnology 2014 Oct;32(10):506-12. Schoene C, Fierer JO, Bennett SP, Howarth M. SpyTag/SpyCatcher Cyclization Confers Resilience to Boiling on a Mesophilic Enzyme. Angewandte Chemie. 2014 Jun 10;53(24):6101-4. Fierer JO, Veggiani G, Howarth M. SpyLigase peptide-peptide ligation polymerizes affibodies to enhance magnetic cancer cell capture. Proc Natl Acad Sci U S A. 2014 Apr 1;111(13):E1176-81. Li L, Fierer JO, Rapoport TA, Howarth M. Structural analysis and optimization of the covalent association between SpyCatcher and a peptide tag. Journal of Molecular Biology. 2014 Jan 23;426(2):309-17. Min, Duyoung; Arbing, Mark A; Jefferson, Robert E; Bowie, James U. A simple DNA handle attachment method for single molecule mechanical manipulation experiments.Protein Sci. 2016 01;25(8):1535-44 View ArticleDovala, Dustin; Sawyer, William S; Rath, Christopher M; Metzger, Louis E. Rapid analysis of protein expression and solubility with the SpyTag-SpyCatcher system.Protein Expr Purif. 2016 01;117:44-51 View ArticleAlves, Nathan J; Turner, Kendrick B; Daniele, Michael A; Oh, Eunkeu; Medintz, Igor L; Walper, Scott A. Bacterial Nanobioreactors--Directing Enzyme Packaging into Bacterial Outer Membrane Vesicles.ACS Appl Mater Interfaces. 2015 11 11;7(44):24963-72 View ArticleReddington, Samuel C; Howarth, Mark. Secrets of a covalent interaction for biomaterials and biotechnology: SpyTag and SpyCatcher.Curr Opin Chem Biol. 2015 12;29:94-9 View ArticleJanitzek, Christoph M; Matondo, Sungwa; Thrane, Susan; Nielsen, Morten A; Kavishe, Reginald; Mwakalinga, Steve B; Theander, Thor G; Salanti, Ali; Sander, Adam F. Bacterial superglue generates a full-length circumsporozoite protein virus-like particle vaccine capable of inducing high and durable antibody responses.Malar J. 2016 11 08;15(1):545 View ArticleSi, Meng; Xu, Qing; Jiang, Ling; Huang, He. SpyTag/SpyCatcher Cyclization Enhances the Thermostability of Firefly Luciferase.PLoS One. 2016;11(9):e0162318 View ArticleZhang, Wen-Bin; Sun, Fei; Tirrell, David A; Arnold, Frances H. Controlling macromolecular topology with genetically encoded SpyTag-SpyCatcher chemistry.J Am Chem Soc. 2013 09 18;135(37):13988-97 View ArticleFairhead, Michael; Veggiani, Gianluca; Lever, Melissa; Yan, Jun; Mesner, Dejan; Robinson, Carol V; Dushek, Omer; Van der Merwe, P Anton; Howarth, Mark. SpyAvidin hubs enable precise and ultrastable orthogonal nanoassembly.J Am Chem Soc. 2014 09 03;136(35):12355-63 View ArticleTan, Lee Ling; Hoon, Shawn S; Wong, Fong T. Kinetic Controlled Tag-Catcher Interactions for Directed Covalent Protein Assembly.PLoS One. 2016;11(10):e0165074 View ArticleWang, Jindan; Wang, Yilin; Wang, Xinzhe; Zhang, Dandan; Wu, Shuyu; Zhang, Guangya. Enhanced thermal stability of lichenase from Bacillus subtilis 168 by SpyTag/SpyCatcher-mediated spontaneous cyclization.Biotechnol Biofuels. 2016;9:79 View ArticleThrane, Susan; Janitzek, Christoph M; Matondo, Sungwa; Resende, Mafalda; Gustavsson, Tobias; De Jongh, Willem Adriaan; Clemmensen, Stine; Roeffen, Will; Van de Vegte-Bolmer, Marga; Van Gemert, Geert Jan; Sauerwein, Robert; Schiller, John T; Nielsen, Morten A; Theander, Thor G; Salanti, Ali; Sander, Adam F. Bacterial superglue enables easy development of efficient virus-like particle based vaccines.J Nanobiotechnology. 2016 04 27;14:30 View ArticleBedbrook, Claire N; Kato, Mihoko; Ravindra Kumar, Sripriya; Lakshmanan, Anupama; Nath, Ravi D; Sun, Fei; Sternberg, Paul W; Arnold, Frances H; Gradinaru, Viviana. Genetically Encoded Spy Peptide Fusion System to Detect Plasma Membrane-Localized Proteins In Vivo.Chem Biol. 2015 08 20;22(8):1108-21 View ArticleSun, Fei; Zhang, Wen-Bin; Mahdavi, Alborz; Arnold, Frances H; Tirrell, David A. Synthesis of bioactive protein hydrogels by genetically encoded SpyTag-SpyCatcher chemistry.Proc Natl Acad Sci U S A. 2014 08 05;111(31):11269-74 View ArticleBrune, Karl D; Leneghan, Darren B; Brian, Iona J; Ishizuka, Andrew S; Bachmann, Martin F; Draper, Simon J; Biswas, Sumi; Howarth, Mark. Plug-and-Display: decoration of Virus-Like Particles via isopeptide bonds for modular immunization.Sci Rep. 2016 01 19;6:19234 View ArticleVeggiani, Gianluca; Nakamura, Tomohiko; Brenner, Michael D; Gayet, Raphaël V; Yan, Jun; Robinson, Carol V; Howarth, Mark. Programmable polyproteams built using twin peptide superglues.Proc Natl Acad Sci U S A. 2016 02 02;113(5):1202-7 View ArticleSchoene, Christopher; Bennett, S Paul; Howarth, Mark. SpyRing interrogation: analyzing how enzyme resilience can be achieved with phytase and distinct cyclization chemistries.Sci Rep. 2016 02 10;6:21151 View ArticleBrune, Karl; Buldun, Can; Li, Yuanyuan; Taylor, Iona; Brod, Florian; Biswas, Sumi; Howarth, Mark. Dual plug-and-display synthetic assembly using orthogonal reactive proteins for twin antigen immunization.Bioconjug Chem. 2017 04 24; View ArticleGao, Xiaoye; Fang, Jie; Xue, Bin; Fu, Linglan; Li, Hongbin. Engineering Protein Hydrogels Using SpyCatcher-SpyTag Chemistry.Biomacromolecules. 2016 09 12;17(9):2812-9 View ArticleSchoene, C; Bennett, S P; Howarth, M. SpyRings Declassified: A Blueprint for Using Isopeptide-Mediated Cyclization to Enhance Enzyme Thermal Resilience.Methods Enzymol. 2016;580:149-67 View ArticleBotyanszki, Zsofia; Tay, Pei Kun R; Nguyen, Peter Q; Nussbaumer, Martin G; Joshi, Neel S. Engineered catalytic biofilms: Site-specific enzyme immobilization onto E. coli curli nanofibers.Biotechnol Bioeng. 2015 10;112(10):2016-24 View ArticleLiu, Zhida; Zhou, Hang; Wang, Wenjun; Tan, Wenjie; Fu, Yang-Xin; Zhu, Mingzhao. A novel method for synthetic vaccine construction based on protein assembly.Sci Rep. 2014 12 01;4:7266 View ArticlePröschel, Marlene; Detsch, Rainer; Boccaccini, Aldo R; Sonnewald, Uwe. Engineering of Metabolic Pathways by Artificial Enzyme Channels.Front Bioeng Biotechnol. 2015;3:168 View ArticleSiegmund, Vanessa; Piater, Birgit; Zakeri, Bijan; Eichhorn, Thomas; Fischer, Frank; Deutsch, Carl; Becker, Stefan; Toleikis, Lars; Hock, Björn; Betz, Ulrich a K; Kolmar, Harald. Spontaneous Isopeptide Bond Formation as a Powerful Tool for Engineering Site-Specific Antibody-Drug Conjugates.Sci Rep. 2016 12 16;6:39291 View ArticleWang, Hejia Henry; Altun, Burcin; Nwe, Kido; Tsourkas, Andrew. Proximity-Based Sortase-Mediated Ligation.Angew Chem Int Ed Engl. 2017 05 02;56(19):5349-5352 View ArticleAlves, Nathan J; Turner, Kendrick B; Medintz, Igor L; Walper, Scott A. Protecting enzymatic function through directed packaging into bacterial outer membrane vesicles.Sci Rep. 2016 04 27;6:24866 View ArticleChen, Allen Y; Deng, Zhengtao; Billings, Amanda N; Seker, Urartu O S; Lu, Michelle Y; Citorik, Robert J; Zakeri, Bijan; Lu, Timothy K. Synthesis and patterning of tunable multiscale materials with engineered cells.Nat Mater. 2014 05;13(5):515-23 View ArticleRegan, Lynne; Caballero, Diego; Hinrichsen, Michael R; Virrueta, Alejandro; Williams, Danielle M; O'hern, Corey S. Protein design: Past, present, and future.Biopolymers. 2015 07;104(4):334-50 View ArticleLeonard, John D; Narlikar, Geeta J. A nucleotide-driven switch regulates flanking DNA length sensing by a dimeric chromatin remodeler.Mol Cell. 2015 03 05;57(5):850-9 View ArticleLiu, Xueliang; Lopez, Paola A; Giessen, Tobias W; Giles, Michael; Way, Jeffrey C; Silver, Pamela A. Engineering Genetically-Encoded Mineralization and Magnetism via Directed Evolution.Sci Rep. 2016 11 29;6:38019 View ArticleSchmid-Burgk, Jonathan L; Höning, Klara; Ebert, Thomas S; Hornung, Veit. CRISPaint allows modular base-specific gene tagging using a ligase-4-dependent mechanism.Nat Commun. 2016 07 28;7:12338 View ArticleZakeri, Bijan; Lu, Timothy K. Synthetic biology of antimicrobial discovery.ACS Synth Biol. 2013 07 19;2(7):358-72 View ArticleAlam MK, El-Sayed A, Barreto K, Bernhard W, Fonge H, Geyer CR. Site-Specific Fluorescent Labeling of Antibodies and Diabodies Using SpyTag/SpyCatcher System for In Vivo Optical Imaging. Mol Imaging Biol. 2018 Jun 12. View ArticleIf you publish research with this product, please let us know so we can cite your paper.
主要内容
Spytag / Spycatcher系统是一种方便的蛋白质偶联工具,用于不可逆的肽 - 蛋白质连接。它是绑定,标记,固定化和创造新种类蛋白质结构的理想选择。高灯:SpyTAG在结合其遗传编码的合作伙伴间谍运动器时形成自发的酰胺键,其高度兼容性 - SpyTag在极稳定的反应产品的广泛条件下与瞬间反应 - SPYCatcher / SPYTAG复合物在SDS SPYCATCHER中稳定稳定(S49C)版本含有独特的半胱氨酸残留物,用于精确标记用染料或精确附着到表面或珠粒肽与蛋白质的相互作用通常弱。 Spytag是一种遗传编码的肽(SpyTAG003序列RGVPHIVMVDEDKRYK),其在结合其遗传编码的合作伙伴间谍程序时形成自发的酰胺键(使用此处提供的最新版本的SPYCATCHER003)。 SpyTAG003在各种条件下与Spycatcher003反应,并在反应后该产物在SDS中沸腾稳定。 Spycatcher003(S49C)具有独特的半胱氨酸,用于精确标记染料或精确附着到表面或珠粒。从牛津大学Mark Howarth的实验室,博士学位。
厂牌介绍

关于Kerafast Inc.

Kerafast 是一家位于波士顿的试剂公司,其主要使命是为QuanQiu科学界提供易于使用的独特实验室研究工具。我们的产品组合包括细胞系、抗体、小分子、染料等,其中许多在其他地方无法获得。自 2011 年成立以来,来自全球 190 多个机构的研究人员通过我们的在线平台提供了他们的创新试剂,无需通过传统的材料转让协议流程即可快速获取材料。

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