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产品图片
产品别名
货号/SKU
EOX001
货号/规格
0.5mg
库存与交货期
1-2周
人民币价格
8800
试剂海关审批
使用人负责A/B风险申请资质
国外采购
支持/部分需签MTA
厂牌
Kerafast, Inc.
品牌
产品基础信息
From the laboratory of Mark Howarth, PhD, University of Oxford.
产品描述信息
Product Type: | Protein |
Accession ID: | ADT80793.1 |
Source: | Recombinant, expressed in E. coli |
Molecular Weight: | 13.2 kDa (without boiling, traptavidin will run on SDS-PAGE as a tetramer and remain bound to biotin) |
Amino Acid Sequence: |
AEAGITGTWYNQLGSTFIVTAGADGALTGTYESAVGNAEGDYVLTGRYDSAPATDGSGTALGWTVAW KNNYRNAHSATTWSGQYVGGAEARINTQWLLTSGTTEANAWKSTLVGHDTFTKVKPSAAS |
Fusion Tag(s): | None |
Purity: | >98% by SDS PAGE (Purified from inclusion body refolding with selective ammonium sulfate precipitation and then iminobiotin-agarose affinity chromatography.) |
Buffer: | Phosphate buffered saline (PBS) pH 7.4; The protein is highly stable and should be compatible with a wide range of salts, ionic strengths and pH values (certainly 5-9), as well as DMF or DMSO up to 10%. |
Concentration: | 3.36 mg/mL in PBS. |
Comments: | 10-fold lower off-rate from biotin and biotin conjugates compared to streptavidin. Increased thermal stability and mechanical stability of biotin binding. |
Storage: | -80°C, stable for 1-2 weeks when kept at 4°C (avoid multiple freeze-thaw cycles) |
Shipped: | Dry ice |
产品安全信息
Chivers CE, Crozat E, Chu C, Moy VT, Sherratt DJ, Howarth M. A streptavidin variant with slower biotin dissociation and increased mechanostability. Nature Methods 2010 May;7(5):391-93.Chivers CE, Koner AL, Lowe ED, Howarth M. How the biotin-streptavidin interaction was made even stronger: investigation via crystallography and a chimaeric tetramer. Biochemical Journal 2011 Apr 1;435(1):55-63.Fu H, Jiang Y, Yang D, Scheiflinger F, Wong WP, Springer TA. Flow-induced elongation of von Willebrand factor precedes tension-dependent activation. Nat Commun. 2017 Aug 23;8(1):324. View ArticleQing Y, Ionescu SA, Pulcu GS, Bayley H. Directional control of a processive molecular hopper. Science. 2018 Aug 31;361(6405):908-912. View ArticleTalreja D, Cashman SM, Dasari B, Kumar B, Kumar-Singh R. G-quartet oligonucleotide mediated delivery of functional X-linked inhibitor of apoptosis protein into retinal cells following intravitreal injection. Exp Eye Res. 2018 Oct;175:20-31. View ArticleNguyen HC, Talledge N, McCullough J, et al. Membrane constriction and thinning by sequential ESCRT-III polymerization. Nat Struct Mol Biol. 2020;27(4):392-399.View articleIf you publish research with this product, please let us know so we can cite your paper.
主要内容
Traptavidin是链霉抗生物素蛋白的S52G,R53D突变体,高亲和力生物素结合蛋白。在各种成像,蛋白质纯化和纳米组件中有用。高灯:与Biotincincincriot的热稳定性和Biotin结合的有效热素结合相比,从生物素和生物素结合的机械稳定性与细胞低的非特异性结合相比,与Biotin结合的机械稳定性相比,从生物素和生物素结合的机械稳定性降低了10倍。 Dnastrepavidin与卓越的稳定性结合生物素缀合物,但是发生解离并且可以限制成像,DNA扩增和纳米技术。与链霉抗生物素蛋白相比,这种Traptavidin蛋白克服了〜10倍的速度较慢的速率,增加的机械强度和改善的热稳定性。从牛津大学Mark Howarth的实验室,博士学位。
厂牌介绍
关于Kerafast Inc.
Kerafast 是一家位于波士顿的试剂公司,其主要使命是为QuanQiu科学界提供易于使用的独特实验室研究工具。我们的产品组合包括细胞系、抗体、小分子、染料等,其中许多在其他地方无法获得。自 2011 年成立以来,来自全球 190 多个机构的研究人员通过我们的在线平台提供了他们的创新试剂,无需通过传统的材料转让协议流程即可快速获取材料。
我们处理提供实验室的所有销售和运输物流,并从每次销售中返还丰厚的特许权使用费。因此,我们帮助提供实验室节省时间和资源,同时为进一步研究提供额外资金。采购科学家可以更轻松地发现和获取其他地方通常无法获得的独特试剂,同时还可以资助其他研究人员的工作。这创建了一个QuanQiu科学家社区,他们贡献和获取Reagent for the Greater Good,以加速他们自己的研究以及整体科学进步。
2018 年,Kerafast 与Absolute Antibody合并,后者是一家总部位于英国的公司,其愿景是为所有研究人员提供重组抗体技术。此次合并将两家公司聚集在一起,共同致力于改善科学界可用的研究工具的选择。
品牌标识
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