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产品图片

产品英文名称

Retinal Muller Cell Line rMC-1

产品别名

Kerafast独特的生物试剂

货号/SKU

ENW001

货号/规格

1 vial

库存与交货期

1-2周

人民币价格

17785

人民币价格说明

本商品人民币2024年销售价格正在调整中，请等待更新完毕。

本商品的展示的人民币价格已包含商品本身金额、VAT增值税13%、国际运输运费、国内物流运费、运输保险、以及冷链包装材料（例如液氮罐、泡沫箱、金属桶、蓝冰、湿冰、干冰、蓄冷剂、液氮等）、装卸费、相关资料费、人力支出等一切费用。

本商品的美元价、市场价、零售价、厂商指导价或该商品的曾经展示过的销售价等，并非商品原价，仅供参考。

试剂海关审批

使用人负责A/B风险申请资质

国外采购

支持/部分需签MTA

厂牌

Kerafast, Inc.

品牌

Kerafast®

产品基础信息

From the laboratory of Vijay Sarthy, PhD, Northwestern University.

产品描述信息

Product Type:	Cell Line
Name:	rMC-1
Cell Type:	Retinal Müller (glial) cell
Accession ID:	CVCL_8140
Organism:	Rat
Source:	Retina
Morphology:	Flat
Biosafety Level:	BSL1
Subculturing:	Harvest cells with 0.05% trypsin for 5 min and replate
Growth Conditions:	DMEM (high glucose, (+) L-Glutamine, (-) Sodium pyruvate) with 10% FBS and 1X pen/strep, with additional L-glutamine (2mM).
Cryopreservation:	DMEM with 10% DMSO and 10%FBS
Storage:	Liquid nitrogen
Shipped:	Dry ice

产品安全信息

Sarthy, V., S. Brodjian, K. Dutt, B. Kennedy, R. French and J. Crabb. Establishment and characterization of a retinal Muller cell line. Invest. Ophthalmol. Vis. Sci. 1998 Jan;39: 212-216.Kannan R, Bao Y, Wang Y, Sarthy VP, Kaplowitz N.Protection from oxidant injury by sodium-dependent GSH uptake in retinal Müller cells. Exp Eye Res. 1999 May;68(5):609-16.Lu SC1, Bao Y, Huang ZZ, Sarthy VP, Kannan R. Regulation of gamma-glutamylcysteine synthetase subunit gene expression in retinal Müller cells by oxidative stress. Invest Ophthalmol Vis Sci. 1999 Jul;40(8):1776-82.Kusner LL, Sarthy VP, Mohr S. Nuclear translocation of glyceraldehyde-3-phosphate dehydrogenase: a role in high glucose-induced apoptosis in retinal Müller cells. Invest Ophthalmol Vis Sci. 2004 May;45(5):1553-61.Du Y, Sarthy VP, Kern TS. Interaction between NO and COX pathways in retinal cells exposed to elevated glucose and retina of diabetic rats. Am J Physiol Regul Integr Comp Physiol. 2004 Oct;287(4):R735-41.Loewen N, Chen J, Dudley VJ, Sarthy VP, Mathura JR Jr. Genomic response of hypoxic Müller cells involves the very low density lipoprotein receptor as part of an angiogenic network.Exp Eye Res. 2009 May;88(5):928-37.Xue W, Du P, Lin S, Dudley VJ, Hernandez MR, Sarthy VP. Gene expression changes in retinal Müller (glial) cells exposed to elevated pressure. Curr Eye Res. 2011 Aug;36(8):754-67.Trueblood KE, Mohr S, Dubyak GR. Purinergic regulation of high-glucose-induced caspase-1 activation in the rat retinal Müller cell line rMC-1. Am J Physiol Cell Physiol. 2011 Nov;301(5):C1213-23. doi: 10.1152/ajpcell.00265.2011. Epub 2011 Aug 10. PubMed PMID: 21832250.Muto T, Tien T, Kim D, Sarthy VP, Roy S. High glucose alters cx43 expression and gap junction intercellular communication in retinal müller cells: promotes müller cell and pericyte apoptosis. Invest Ophthalmol Vis Sci. 2014 Jun 17;55(7):4327-37.Pfeffer BA, Xu L, Porter NA, Rao SR, Fliesler SJ. Differential cytotoxic effects of 7-dehydrocholesterol-derived oxysterols on cultured retina-derived cells: Dependence on sterol structure, cell type, and density. Exp Eye Res. 2016 Feb 12;145:297-316.Xi H, Katschke KJ Jr, Li Y, Truong T, Lee WP, Diehl L, Rangell L, Tao J, Arceo R, Eastham-Anderson J, Hackney JA, Iglesias A, Cote-Sierra J, Elstrott J, Weimer RM, van Lookeren Campagne M. IL-33 amplifies an innate immune response in the degenerating retina. J Exp Med. 2016 Feb 8;213(2):189-207. doi: 10.1084/jem.20150894. PubMed PMID: 26755704; PubMed Central PMCID: PMC4749925. View ArticleMishra S, Vazquez M. A Gal-MµS Device to Evaluate Cell Migratory Response to Combined Galvano-Chemotactic Fields. Biosensors (Basel). 2017 Nov 21;7(4). pii: E54. View ArticleTien T, Zhang J, Muto T, Kim D, Sarthy VP, Roy S. High Glucose Induces Mitochondrial Dysfunction in Retinal Müller Cells: Implications for Diabetic Retinopathy. Invest Ophthalmol Vis Sci. 2017 Jun 1;58(7):2915-2921. doi: 10.1167/iovs.16-21355. PubMed PMID: 28586916; PubMed Central PMCID: PMC5460955.Picconi F, Parravano M, Sciarretta F, Fulci C, Nali M, Frontoni S, Varano M, Maria Caccuri A. Activation of retinal Müller cells in response to glucose variability. Endocrine. 2019 Jul 20. View ArticlePeña JS, Robles D, Zhang S, Vazquez M. A Milled Microdevice to Advance Glia-Mediated Therapies in the Adult Nervous System. Micromachines (Basel). 2019 Jul 31;10(8). View ArticleMa M, Zhao S, Zhang J, Sun T, Fan Y, Zheng Z. High Glucose-Induced TRPC6 Channel Activation Decreases Glutamate Uptake in Rat Retinal Müller Cells. Front Pharmacol. 2020;10:1668. Published 2020 Feb 14.View articlePeña JS, Vazquez M. VEGF Upregulates EGFR Expression to Stimulate Chemotactic Behaviors in the rMC-1 Model of Müller Glia. Brain Sci. 2020;10(6):E330. Published 2020 May 29.View articleStanchfield ML, Webster SE, Webster MK, Linn CL. Involvement of HB-EGF/Ascl1/Lin28a Genes in Dedifferentiation of Adult Mammalian Müller Glia. Front Mol Biosci. 2020 Aug 14;7:200.View artcileIf you publish research with this product, please let us know so we can cite your paper.

主要内容

RMC-1永生化细胞组成型表达Müller细胞中存在的GFAP（胶质纤维酸性蛋白质）和CRALBP（细胞转基因醛结合蛋白），其存在于Müller细胞中.Highlights：组成型表达GFAP和CRALBP通过从成年大鼠视网膜中转染原发性müller细胞培养物而永生化病毒oncogene表达诱导和基础标记的诱导和基础标志物在原发性Müller细胞培养物中发现，对于进行的糖尿病视网膜病细胞系验证的临床前研究非常有用 - 请参阅：Pfeffer Ba等。 exp眼睛。 2016年2月12日; 145：297-316.Müller细胞是脊椎动物视网膜中发现的最丰富的胶质细胞，并对支持视网膜神经元的活动进行多样化功能。这种永生化的细胞系有助于Müller细胞中的基因表达研究，提高了对Müller细胞 - 神经元相互作用的理解。从西北大学Vijay Sarthy的实验室。

厂牌介绍

关于Kerafast Inc.

Kerafast 是一家位于波士顿的试剂公司，其主要使命是为QuanQiu科学界提供易于使用的独特实验室研究工具。我们的产品组合包括细胞系、抗体、小分子、染料等，其中许多在其他地方无法获得。自 2011 年成立以来，来自全球 190 多个机构的研究人员通过我们的在线平台提供了他们的创新试剂，无需通过传统的材料转让协议流程即可快速获取材料。

我们处理提供实验室的所有销售和运输物流，并从每次销售中返还丰厚的特许权使用费。因此，我们帮助提供实验室节省时间和资源，同时为进一步研究提供额外资金。采购科学家可以更轻松地发现和获取其他地方通常无法获得的独特试剂，同时还可以资助其他研究人员的工作。这创建了一个QuanQiu科学家社区，他们贡献和获取Reagent for the Greater Good，以加速他们自己的研究以及整体科学进步。

2018 年，Kerafast 与Absolute Antibody合并，后者是一家总部位于英国的公司，其愿景是为所有研究人员提供重组抗体技术。此次合并将两家公司聚集在一起，共同致力于改善科学界可用的研究工具的选择。