4 次围观

产品图片

产品英文名称

Canine Hemangiosarcoma Cell Line (DD-1)

产品别名

Kerafast独特的生物试剂

货号/SKU

EMN009-FP

货号/规格

1 vial

库存与交货期

1-2周

人民币价格

14050

人民币价格说明

本商品人民币2024年销售价格正在调整中，请等待更新完毕。

本商品的展示的人民币价格已包含商品本身金额、VAT增值税13%、国际运输运费、国内物流运费、运输保险、以及冷链包装材料（例如液氮罐、泡沫箱、金属桶、蓝冰、湿冰、干冰、蓄冷剂、液氮等）、装卸费、相关资料费、人力支出等一切费用。

本商品的美元价、市场价、零售价、厂商指导价或该商品的曾经展示过的销售价等，并非商品原价，仅供参考。

试剂海关审批

使用人负责A/B风险申请资质

国外采购

支持/部分需签MTA

厂牌

Kerafast, Inc.

品牌

Kerafast®

产品基础信息

From the laboratory of Jaime F. Modiano, VMD, PhD, University Minnesota, Twin Cities.

产品描述信息

Product Type:	Cell Line
Name:	DD1
Cell Type:	Hemangiosarcoma
Organism:	Dog
Accession ID:	CVCL_0C94
Morphology:	Spindle
Source:	Spleen
Biosafety Level:	2
Growth Conditions:	Ham's F12, 10% FBS, 0.05mg/ml ECGS, 0.01mg/ml heparin, 10mM HEPES Buffer, 100ug/ml Primocin
Subculturing:	Split 1:3-1:5 when cells reach ~80% confluence.
Cryopreservation:	90%FBS/10% DMSO
Mycoplasma Tested:	Yes
Storage:	Liquid nitrogen
Shipped:	Dry ice

产品安全信息

Fosmire SP, Dickerson EB, Scott AM, Bianco SR, Pettengill MJ, Meylemans H, Padilla M, Frazer-Abel AA, Akhtar N, Getzy DM, Wojcieszyn J, Breen M, Helfand SC, Modiano JF. Canine malignant hemangiosarcoma as a model of primitive angiogenic endothelium. Lab Invest. 2004 May;84(5):562-72. Frazer-Abel AA, Baksh S, Fosmire SP, Willis D, Pierce AM, Meylemans H, Linthicum DS, Burakoff SJ, Coons T, Bellgrau D, Modiano JF. Nicotine activates nuclear factor of activated T cells c2 (NFATc2) and prevents cell cycle entry in T cells. J Pharmacol Exp Ther. 2004 Nov;311(2):758-69. Epub 2004 Jul 1. Dickerson EB, Thomas R, Fosmire SP, Lamerato-Kozicki AR, Bianco SR, Wojcieszyn JW, Breen M, Helfand SC, Modiano JF. Mutations of phosphatase and tensin homolog deleted from chromosome 10 in canine hemangiosarcoma. Vet Pathol. 2005 Sep;42(5):618-32. Lamerato-Kozicki AR, Helm KM, Jubala CM, Cutter GC, Modiano JF. Canine hemangiosarcoma originates from hematopoietic precursors with potential for endothelial differentiation. Exp Hematol. 2006 Jul;34(7):870-8. Tamburini BA, Trapp S, Phang TL, Schappa JT, Hunter LE, Modiano JF. Gene expression profiles of sporadic canine hemangiosarcoma are uniquely associated with breed. PLoS One. 2009 May 20;4(5):e5549. doi: 10.1371/journal.pone.0005549. Tamburini BA, Phang TL, Fosmire SP, Scott MC, Trapp SC, Duckett MM, Robinson SR, Slansky JE, Sharkey LC, Cutter GR, Wojcieszyn JW, Bellgrau D, Gemmill RM, Hunter LE, Modiano JF. Gene expression profiling identifies inflammation and angiogenesis as distinguishing features of canine hemangiosarcoma. BMC Cancer. 2010 Nov 9;10:619. doi: 10.1186/1471-2407-10-619. Schappa JT, Frantz AM, Gorden BH, Dickerson EB, Vallera DA, Modiano JF. Hemangiosarcoma and its cancer stem cell subpopulation are effectively killed by a toxin targeted through epidermal growth factor and urokinase receptors. Int J Cancer. 2013 Oct 15;133(8):1936-44. doi: 10.1002/ijc.28187. Epub 2013 Apr 25. Gorden BH, Kim JH, Sarver AL, Frantz AM, Breen M, Lindblad-Toh K, O'Brien TD, Sharkey LC, Modiano JF, Dickerson EB. Identification of three molecular and functional subtypes in canine hemangiosarcoma through gene expression profiling and progenitor cell characterization. Am J Pathol. 2014 Apr;184(4):985-95. doi: 10.1016/j.ajpath.2013.12.025. Epub 2014 Feb 11. Kim JH, Frantz AM, Anderson KL, Graef AJ, Scott MC, Robinson S, Sharkey LC, O'Brien TD, Dickerson EB, Modiano JF. Interleukin-8 promotes canine hemangiosarcoma growth by regulating the tumor microenvironment. Exp Cell Res. 2014 Apr 15;323(1):155-64. doi: 10.1016/j.yexcr.2014.02.020. Epub 2014 Feb 25. Gorden BH, Saha J, Khammanivong A, Schwartz GK, Dickerson EB. Lysosomal drug sequestration as a mechanism of drug resistance in vascular sarcoma cells marked by high CSF-1R expression. Vasc Cell. 2014 Oct 1;6:20. doi: 10.1186/2045-824X-6-20. eCollection 2014. Rodriguez AM, Graef AJ, LeVine DN, Cohen IR, Modiano JF, Kim JH. Association of Sphingosine-1-phosphate (S1P)/S1P Receptor-1 Pathway with Cell Proliferation and Survival in Canine Hemangiosarcoma. J Vet Intern Med. 2015 Jul;29(4):1088-97. doi: 10.1111/jvim.13570. Epub 2015 Jun 25.Lin W, Modiano JF, Ito D. Stage-specific embryonic antigen: determiningexpression in canine glioblastoma, melanoma, and mammary cancer cells. J Vet Sci.2017 Mar 30;18(1):101-104. doi: 10.4142/jvs.2017.18.1.101. PubMed PMID: 27456773;PubMed Central PMCID: PMC5366293.Alfaleh MA, et al. Canine CD117-Specific Antibodies with Diverse Binding Properties Isolated from a Phage Display Library Using Cell-Based Biopanning. Antibodies. 2019;8(1):15. View ArticleIf you publish research with this product, please let us know so we can cite your paper.

主要内容

DD1是一种甘蔗血管瘤细胞系，源于11岁的男性金毛猎犬/大型比利牛斯混合物中的脾脏.Hemangiosarcoma是一种迅速增长的，高度侵入性的癌症，通常在狗中发生。这是一种由血管衬里引起的肉瘤。经常死亡的死因是这种肿瘤的破裂，导致患者迅速渗出死亡。从jaime f. modiano，vmd，博士，明尼苏达大学，孪生城市的实验室。

厂牌介绍

关于Kerafast Inc.

Kerafast 是一家位于波士顿的试剂公司，其主要使命是为QuanQiu科学界提供易于使用的独特实验室研究工具。我们的产品组合包括细胞系、抗体、小分子、染料等，其中许多在其他地方无法获得。自 2011 年成立以来，来自全球 190 多个机构的研究人员通过我们的在线平台提供了他们的创新试剂，无需通过传统的材料转让协议流程即可快速获取材料。

我们处理提供实验室的所有销售和运输物流，并从每次销售中返还丰厚的特许权使用费。因此，我们帮助提供实验室节省时间和资源，同时为进一步研究提供额外资金。采购科学家可以更轻松地发现和获取其他地方通常无法获得的独特试剂，同时还可以资助其他研究人员的工作。这创建了一个QuanQiu科学家社区，他们贡献和获取Reagent for the Greater Good，以加速他们自己的研究以及整体科学进步。

2018 年，Kerafast 与Absolute Antibody合并，后者是一家总部位于英国的公司，其愿景是为所有研究人员提供重组抗体技术。此次合并将两家公司聚集在一起，共同致力于改善科学界可用的研究工具的选择。