Westermann S, Wang HW, Avila-Sakar A, Drubin DG, Nogales E and Barnes G. The kinetochore ring complex moves processively on depolymerizing microtubule ends. NATURE 440:565-563, 2006

root 提交于 周日, 10/31/2021 - 01:47
Abstract Chromosomes interact through their kinetochores with microtubule plus ends and they are segregated to the spindle poles as the kinetochore microtubules shorten during anaphase A of mitosis. The molecular natures and identities of coupling proteins that allow microtubule depolymerization to pull chromosomes to poles during anaphase have long remained elusive. In budding yeast, the ten-protein Dam1 complex is a critical microtubule-binding component of the kinetochore that oligomerizes into a 50-nm ring around a microtubule in vitro. Here we show, with the use of a real-time, two-colour fluorescence microscopy assay, that the ring complex moves processively for several micrometres at the ends of depolymerizing microtubules without detaching from the lattice. Electron microscopic an......

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