柠檬酸钠缓冲粉(BZ213)500ml

产品图片
产品别名
货号/SKU
Chemazone654
库存与交货期
1-2周
人民币价格
4743
人民币价格说明
本商品人民币2024年销售价格正在调整中,请等待更新完毕。

本商品的展示的人民币价格已包含商品本身金额、VAT增值税13%、国际运输运费、国内物流运费、运输保险、以及冷链包装材料(例如液氮罐、泡沫箱、金属桶、蓝冰、湿冰、干冰、蓄冷剂、液氮等)、装卸费、相关资料费、人力支出等一切费用。

本商品的美元价、市场价、零售价、厂商指导价或该商品的曾经展示过的销售价等,并非商品原价,仅供参考。

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厂牌
Chemazone Inc
产品基础信息
1000mL, 100mL, 200mL, 500mL
产品描述信息

Product name Sodium Citrate Buffer with Powder (0.01mol/L, pH6.0) / Sodium Citrate Buffer with Powder (0.01mol/L, pH6.0)
Product specifications 1L | 2L

reagents are stored at room temperature and sealed, and the expiry date is at least 24 months.

Product introduction After cells or tissues are fixed with paraformaldehyde, formaldehyde or other aldehyde reagents, the intracellular antigens will form aldehyde bonds and carboxymethyl bonds, which will block part of the antigenic determinants, and at the same time cross-link between the proteins to make the antigen The determinants are concealed, leading to weakening of the staining signal during immunostaining, and even some false negative staining results. Therefore, it is required that when performing immunohistochemical staining, it is necessary to perform antigen retrieval or exposure first, that is, to destroy the crosslinks formed between molecules during fixation, and restore the original spatial shape of the antigen. Thereby increasing the detection rate of antigen, reducing background staining, and improving the accuracy of detection. Sodium citrate buffer 0.01mol/L, pH 6.0 is a commonly used antigen retrieval solution, which can be used for antigen retrieval after paraffin section, frozen section and other samples are fixed with paraformaldehyde, formaldehyde or other aldehyde reagents. It can effectively remove the crosslinks between proteins caused by aldehyde fixation reagents, and fully expose the epitopes in samples such as paraffin sections, thereby greatly improving the immunostaining effect. Normally, paraffin sections need to be processed for antigen retrieval, while frozen sections need not be processed for antigen retrieval. Antigen retrieval can improve the immunostaining effect of paraffin sections, and can also improve the staining effect of frozen sections to varying degrees. When the immunostaining effect of frozen sections is not satisfactory, consider antigen retrieval. According to the 10ml antigen retrieval solution required for each film, 1L of antigen retrieval solution can be used for antigen retrieval of 100 samples. Reference for Use This product is in dry powder form. Dissolve it to 1L or 2L in deionized water or distilled water according to the product specifications before use, and it can be used.

1. For paraffin sections:

a. Deparaffinization: xylene 3 times, 3-5 min each time → absolute ethanol 2 times, 3-5 min each time → 95% ethanol 1 time, 3-5 min → 90% ethanol 1 time, 3-5min→75% ethanol once, 3-5min→wash with distilled water twice, 3-5min each time.

b. Antigen retrieval: Immerse the slices in citric acid retrieval solution, and heat at 95-100℃ for about 15 minutes (The heating time can be controlled within 10-20 minutes. The best heating time should be explored according to different samples and target proteins. ). The citric acid repair solution needs to be preheated to 95-100°C before use. You can use an ordinary water bath or a microwave oven for heating. If you use a microwave oven for heating, you need to be careful to avoid bumping and excessive evaporation of water. Then cool to room temperature in approximately 20-30 minutes. Wash 1-2 times with immunostaining washing solution for 3-5 min each time. Subsequently, subsequent immunostaining steps such as blocking can be performed.

2. For frozen sections: Wash the sections with immunostaining washing solution for 5 minutes. Soak the slices in citric acid repair solution and heat at 95-100°C for about 15 minutes (heating time can be controlled within 10-20 minutes, and the best heating time needs to be explored according to different samples and target proteins). The citric acid repair solution needs to be preheated to 95-100°C before use. You can use an ordinary water bath or a microwave oven for heating. If you use a microwave oven for heating, take care to avoid bumping and excessive evaporation of water. Then cool to room temperature in approximately 20-30 minutes. Wash 1-2 times with immunostaining washing solution for 3-5 min each time. Subsequently, subsequent immunostaining steps such as blocking can be performed.

3. For antigen retrieval of other samples, refer to the steps of paraffin section or frozen section.

Matters needing attention For your safety and health, please wear laboratory clothes and gloves for operation.

产品安全信息
以下中文仅供参考,如专业术语有误,请以英文为准!###产品名称柠檬酸钠缓冲液,粉末(0.01mol / L,pH6.0)/柠檬酸钠缓冲液,粉末(0.01mol / L,pH6.0) 产品规格1L | 2L. 试剂在室温下储存并密封,到期日至少为24个月。 产品引入细胞或组织用多聚甲醛,甲醛或其他醛试剂固定后,细胞内抗原将形成醛键和羧甲基键,其将阻断部分抗原决定因素,同时在蛋白质之间交联决定因素被隐藏的抗原导致免疫染色期间染色信号的弱化,甚至一些假阴性染色结果。因此,需要在进行免疫组织化学染色时,首先需要进行抗原检索或暴露,即,以破坏固定期间在分子之间形成的交联,并恢复原始空间形状的抗原。从而提高抗原的检出率,减少背景染色,提高检测精度。柠檬酸钠缓冲液0.01mol / L,pH6.0是常用的抗原检索溶液,可用于在石蜡切片后的抗原检索,冷冻部分和其他样品用多聚甲醛,甲醛或其他醛试剂固定。它可以有效地除去由醛固定试剂引起的蛋白质之间的交联,并完全暴露在诸如石蜡切片的样品中的表位,从而大大提高了免疫染色效果。通常,需要将石蜡切片用于抗原检索,而冷冻部分不需要处理抗原检索。抗原检索可以改善石蜡切片的免疫染色作用,还可以改善冷冻切片对不同程度的染色效果。当冷冻部分的免疫染色效果不令人满意时,考虑抗原检索。根据每膜所需的10ml抗原检索溶液,1L抗原检索溶液可用于100个样品的抗原检索。参考用本产品处于干粉形式。在使用前根据产品规格将其溶解至去离子水或蒸馏水中的1L或2L,可以使用。 1.对于石蜡部分: 一个。羟甲烷化:二甲苯3次,每次3-5分钟,每次3-5分钟,每次3-5分钟→95%乙醇1时间,3-5分钟→90%乙醇1次,3-5min→75%乙醇一次,3-5分钟→用蒸馏水洗涤两次,每次3-5min。 湾抗原检索:将切片浸入柠檬酸检索溶液中,并在95-100℃下加热约15分钟(加热时间可以在10-20分钟内控制。应根据不同的样品和靶蛋白探索最佳加热时间。)。柠檬酸修复溶液在使用前需要预热至95-100°C。您可以使用普通的水浴或微波炉进行加热。如果您使用微波炉进行加热,则需要小心避免碰撞和过度蒸发水。然后在大约20-30分钟内冷却至室温。每次用免疫洗涤溶液洗涤1-2次,每次3-5分钟。随后,可以进行随后的免疫染色步骤,例如阻塞。 2.对于冷冻部分:用免疫染色洗涤溶液洗涤5分钟的截面。将柠檬酸修复溶液中的切片浸泡在95-100℃下加热约15分钟(可以在10-20分钟内控制加热时间,并且需要根据不同的样品和靶蛋白探索最佳加热时间)。柠檬酸修复溶液在使用前需要预热至95-100°C。您可以使用普通的水浴或微波炉进行加热。如果您使用微波炉进行加热,请注意避免碰撞和过度蒸发水。然后在大约20-30分钟内冷却至室温。每次用免疫洗涤溶液洗涤1-2次,每次3-5分钟。随后,可以进行随后的免疫染色步骤,例如阻塞。 3.对于其他样品的抗原检索,请参阅石蜡部分或冷冻部分的步骤。 需要注意您的安全和健康的事项,请佩戴实验室衣服和手套进行操作。可以根据要求提供柠檬酸钠缓冲液粉末缓冲液组合物和顾客; S指定的配置。稳定剂,pH值,包装尺寸, 柠檬酸钠缓冲液粉末和可定制的含量。 联系我们进行定制:Sales@coreab.cn 运输和储存:室温下的运输,4℃储存。对于长期储存在-20℃下冻结它
主要内容

Sodium citrate buffer powder Buffer composition and customer’s specified configurations can be delivered upon request. Stabilizer, pH Value, Pack Size,

Sodium citrate buffer powder and Contents of are customizable.
Contact us for Customization: sales@coreab.cn
Transportation and storage: Transportation at room temperature, storage at 4 ℃. For Longterm Storage Freeze it at -20 ℃

厂牌介绍

关于 Biochemazone™

人工模拟生物体液试剂和仪器的先驱

Biochemazone™是一家不断发展的生物科技公司,提供高品质、纯化的人工生物化学品、试剂和解决方案,如模拟唾液、模拟尿液、模拟汗液等,并开发学术研究单位、大学、医院、工业、政府和私人实验室。

我们的生化试剂经过全面测试,可满足对卓越品质、不妥协生物的研发需求,以具有竞争力的价格准确模拟真正的生物解决方案活动。

Biochemazone™在科学界建立了良好的声誉,其声誉建立在创新产品、客户服务和对细节的关注上。我们的质量声誉是在市场上取得有利地位以及与制造顶级合成生物产品(如人造唾液、人造尿液、人造皮脂、人造汗液、人造汗液、人造血清、人工血液、人工精液、人工耳垢等等……

欢迎消费品测试实验室和制造商联系 Biochemazone™或本地代理商以了解人工模拟生物流体液体和生化试剂的需求。

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2024-06-03