NR-52512含有血管紧张素转换酶2基因的载体pHAGE2(质粒/载体）

NR-52512?? Vector pHAGE2 Containing the Angiotensin-Converting Enzyme 2 Gene(Plasmid/Vectors)||Vector pHAGE2 Containing the Angiotensin-Converting Enzyme 2 Gene| -20°C or colder|J BloomAcknowledgment for publications should read “The following reagent was obtained through BEI Resources, NIAID, NIH: Vector pHAGE2 Containing the Angiotensin-Converting Enzyme 2 Gene, NR-52512.”|Quantity limit per order for this item is 1. This item can be ordered twice a year. Orders over this limit will be sent to NIAID for approval before shipment. The vector for the human angiotensin-converting enzyme 2 (ACE2) gene (GenBank: NM_021804) was subcloned into the pHAGE2 lentiviral backbone vector under the human EF1α promoter with an intron to increase expression. In addition, pHAGE2 includes the Woodchuck hepatitis virus post-transcriptional regulatory element to enhance levels of transcription and gene expression. NR-52512 contains the beta-lactamase gene, bla, to provide transformant selection through ampicillin resistance in Escherichia coli (E. coli). The resulting size of the plasmid is approximately 9390 base pairs. The complete plasmid sequence and map are provided on the BEI Resources webpage. The plasmid was produced in E. coli and extracted. NR-52512 is part of a lentiviral expression system, and additional BEI Resources items are required for successful ACE2 expression. Lentiviral expression of ACE2 requires lentiviral helper plasmids (BEI Resources NR-52517, NR-52518 and NR-52519, which are provided in the kit NR-52948). Protocols for the use of these items are published. Note: NR-52512 does not include an antibiotic selection cassette for mammalian expression. Immunocytometry is required to identify cells expressing ACE2. ACE2 is a human receptor expressed widely, including in the heart, kidney, small intestine and lung, and is involved in the regulation of hypertension and other cardiovascular diseases. The SARS-related coronavirus 2 spike glycoprotein mediates viral binding to the host ACE2 receptor. This protein forms a trimer, and when bound to ACE2 allows fusion of the viral and cellular membranes, allowing viral entry and replication. The interaction of ACE2 and the spike glycoprotein during viral infection is currently under study. Each vial contains plasmid DNA in TE buffer (10 mM Tris-HCl, 1 mM EDTA, pH 8.0). The vial should be centrifuged prior to opening. Note: The contents of the vial should be used to replicate the plasmid in E. coli prior to expression studies.