产品图片
货号/SKU
NR-50781
货号/规格
EA
库存与交货期
3-8周
人民币价格
14000
试剂海关审批
A/B级风险物质只能直接使用人购买并持有实验室有效资质，其它询客服确认
国外采购
支持/部分限制一年内购买数量
厂牌
BEI Resources(ATCC)
产品基础信息
生物安全等级建议分类：美国、3
产品描述信息
NR-50781??Mycobacterium tuberculosis, Erdman Barcode Library(Libraries)|Erdman Barcode Library|Mycobacterium tuberculosis|-80°C or colder|S Fortune, D SchnappingerAcknowledgment for publications should read “The following reagent was obtained through BEI Resources, NIAID, NIH: Mycobacterium tuberculosis, Strain Erdman Barcode Library, NR-50781.”|Quantity limit per order for this item is 5. This item can be ordered twice a year. Orders over this limit will be sent to NIAID for approval before shipment.
NR-50781 is a digitally barcoded Mycobacterium tuberculosis (M. tuberculosis), strain Erdman library where each bacterium in the library carries a unique, randomized sequence tag that can be used to quantitatively track the fate of each infecting bacterium. The “barcode” consists of a random 7-mer and adjacent 75-mer library identifier tag inserted at the bacterial attB L5 insertion site of a chromosomally integrating plasmid with pJEB402 backbone. The plasmid was a standard integrating mycobacterial vector containing an Escherichia coli origin of replication, the attP element for insertion into the L5 site and is maintained by a kanamycin resistance cassette. The strain remains susceptible to all first line antimycobacterial agents once the barcode is stably inserted into the bacterial chromosome. To quantitate barcodes via sequencing, the barcode is amplified from isolated genomic DNA in two rounds of nested PCR steps.
The virulence characteristics of the barcoded M. tuberculosis have been assessed by quantitative plating and assessments of pathology in over two dozen nonhuman primates where the barcoded strain has similar virulence characteristics to the parental strain of Erdman. The complete genome of M. tuberculosis, strain Erdman was deposited in the DNA Database of Japan under accession no. AP012340.
Each vial contains approximately 0.7 mL (1.45 × 108 cfu) of M. tuberculosis, strain Erdman barcode library in Middlebrook 7H9 medium with additives (10% oleic acid-dextrose-catalase; 0.2% glycerol; 0.05% Tween? 80 and 20 ?g/mL kanamycin) and cryopreservative (10% glycerol, 0.85% NaCl and 0.05% tyloxapol). The stock can be used directly for infection or can be recovered in broth culture prior to infection, depending on the lab’s infection protocol.
Note: Do not repeatedly culture the same aliquot. This can skew qtag/barcode (qbid) distribution. Expansion of the culture is the same for all but the protocol for infection will vary depending on the route of infection. Users should follow their lab protocol once the culture is expanded.
For-profit entities wishing to obtain this material must obtain permission from the Contributor’s institution. Please contact the Harvard University Licensing Office to obtain permission via email: hms_materialtransfer@harvard.edu.
NR-50781 is a digitally barcoded Mycobacterium tuberculosis (M. tuberculosis), strain Erdman library where each bacterium in the library carries a unique, randomized sequence tag that can be used to quantitatively track the fate of each infecting bacterium. The “barcode” consists of a random 7-mer and adjacent 75-mer library identifier tag inserted at the bacterial attB L5 insertion site of a chromosomally integrating plasmid with pJEB402 backbone. The plasmid was a standard integrating mycobacterial vector containing an Escherichia coli origin of replication, the attP element for insertion into the L5 site and is maintained by a kanamycin resistance cassette. The strain remains susceptible to all first line antimycobacterial agents once the barcode is stably inserted into the bacterial chromosome. To quantitate barcodes via sequencing, the barcode is amplified from isolated genomic DNA in two rounds of nested PCR steps.
The virulence characteristics of the barcoded M. tuberculosis have been assessed by quantitative plating and assessments of pathology in over two dozen nonhuman primates where the barcoded strain has similar virulence characteristics to the parental strain of Erdman. The complete genome of M. tuberculosis, strain Erdman was deposited in the DNA Database of Japan under accession no. AP012340.
Each vial contains approximately 0.7 mL (1.45 × 108 cfu) of M. tuberculosis, strain Erdman barcode library in Middlebrook 7H9 medium with additives (10% oleic acid-dextrose-catalase; 0.2% glycerol; 0.05% Tween? 80 and 20 ?g/mL kanamycin) and cryopreservative (10% glycerol, 0.85% NaCl and 0.05% tyloxapol). The stock can be used directly for infection or can be recovered in broth culture prior to infection, depending on the lab’s infection protocol.
Note: Do not repeatedly culture the same aliquot. This can skew qtag/barcode (qbid) distribution. Expansion of the culture is the same for all but the protocol for infection will vary depending on the route of infection. Users should follow their lab protocol once the culture is expanded.
For-profit entities wishing to obtain this material must obtain permission from the Contributor’s institution. Please contact the Harvard University Licensing Office to obtain permission via email: hms_materialtransfer@harvard.edu.
主要内容
此项目的每个订单数量限制为5.＆nbsp;这个项目可以每年订购两次.＆nbsp;在此限制之前的订单将在发货前发送到NIAID进行批准. NR-50781
是数字条形码的分枝杆菌
结核病（ m.结核病），
菌株埃尔德曼图书馆，图书馆中的每个细菌都带有独特的，
可用于定量追踪命运的随机序列标记
每种感染细菌.＆nbsp; “条形码”包括
随机7-mer和相邻的75-mer库标识符标签插入
细菌attb l5染色体整合质粒的插入部位
PJEB402骨干.这
质粒是含有大肠杆菌＆nbsp的标准分枝杆菌载体; 复制的起源，
用于插入L5位点的ATTP元件并由A保持
卡那霉素抵抗盒.＆nbsp;菌株仍然易于第一次
一旦条形码稳定地插入，抗细菌剂
细菌染色体.通过测序定量条形码，可以扩增条形码
在两轮嵌套PCR步骤中的分离基因组DNA.
条形码的毒力特性 m.
结核病和; 已通过定量电镀和评估进行评估
在两个十几个非人的灵长类动物中的病理学，条形码菌株有
埃尔德曼父母株的类似毒力特征.
M的完整基因组.结核病，菌株埃尔德曼是
存放在日本的DNA数据库中. AP012340. 每个小瓶含有约0.7毫升（1.45×
M的10 8 cfu）.结核病，
菌株Erdman条形码库中的中间体7H9培养基（10％）
油酸 - 右旋糖 - 过氧化氢酶; 0.2％甘油; 0.05％Tween ?＆nbsp; 80和20μg/ ml卡那霉素）和冷冻保存（10％甘油，0.85％NaCl和0.05％
Tyloxapol）.该库存可直接用于感染或
在感染之前可以在肉汤培养中恢复，取决于实验室
感染方案. 注意：不要
反复培养相同的等分试样.这可以偏斜qtag /条形码（qbid）
分布.＆nbsp; 扩展
文化对所有内容相同，但感染议定书将取决于
关于感染途径.用户应遵循其实验协议一次
文化扩大.＆nbsp;
营利实体
希望获得此材料必须获得贡献者的许可
机构.请联系
哈佛大学许可办公室通过电子邮件获取许可：＆nbsp; hms_materialtransfer@harvard.edu .
厂牌介绍
BEI Resources 由美国国家过敏和传染病研究所 ( NIAID )成立，旨在为研究 A、B 和 C 类优先病原体、新兴传染病病原体、非病原微生物和其他相关微生物材料提供试剂、工具和信息到研究界。BEI Resources 获取、验证和生产科学家进行基础研究和开发改进的诊断测试、疫苗和疗法所需的试剂。通过将这些功能集中在 BEI Resources 中，可以监控科学界对这些材料的访问和使用，并确保试剂的质量控制。
除了为传染病界提供材料外，BEI Resources 还鼓励和支持研究人员和机构的材料存放。使用 BEI Resources存放材料对研究人员和研究社区有许多优势，包括安全存储、社区访问和分发；同时保护存款人的知识产权。只要有需要，BEI 资源库将作为研究人员的资源进行维护。您在 BEI Resources 的存款是一项有助于未来研究的长期投资。
BEI Resources 自 2003 年起由美国典型培养物保藏中心 (ATCC) 根据合同管理。2016 年 5 月， ATCC获得了一份为期七年的继续管理 BEI Resources的合同。合同范围已扩大到更全面的研究目录材料，包括由其他政府支持的研究项目存放的材料，将提供给生物防御和新兴传染病科学界。真菌、寄生虫、载体和其他相关材料已添加到现有的细菌、病毒和毒素试剂中，涵盖 NIAID A、B 和 C 类优先病原体和 NIAID 指定的新发传染病病原体和生物。
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