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产品图片

货号/SKU
NR-46429
货号/规格
EA
库存与交货期
3-8周
人民币价格
14000
试剂海关审批
A/B级风险物质只能直接使用人购买并持有实验室有效资质,其它询客服确认
国外采购
支持/部分限制一年内购买数量
厂牌
BEI Resources(ATCC)
产品基础信息
生物安全等级建议分类:美国、2
产品描述信息
NR-46429??Trypanosoma cruzi, Strain Brazil (+luc) (axenic epimastigote)(Parasitic Protozoa)|Trypanosoma cruzi|Strain Brazil (+luc) (axenic epimastigote)|-130°C or colder (Note: Do not under any circumstances store vials at temperatures warmer than -70°C. Storage under these conditions will result in the death of the culture.)|A RodriguezAcknowledgment for publications should read "The following reagent was obtained through BEI Resources, NIAID, NIH: Trypanosoma cruzi, Strain Brazil (+luc) (axenic epimastigote), NR-46429."|Quantity limit per order for this item is 1. This item can be ordered twice a year. Orders over this limit will be sent to NIAID for approval before shipment.
Note: The strain designation on the label is incorrect. The correct strain designation is Brazil (+luc). NR-46429 was deposited to BEI Resources as strain Y (+luc) and updated by the depositor to strain Brazil (+luc).
The epimastigote stage of Trypanosoma cruzi (T. cruzi), strain Brazil (+luc) was established from the trypomastigote form (BEI Resources NR-40347) through multiple passages in culture. The Brazil (+luc) strain is a luciferase-expressing transgenic clone derived from the Brazil strain, which was isolated from a human patient in Brazil.
The luciferase gene was introduced to the Brazil strain of T. cruzi via the pTREX-GFP plasmid where luc replaced GFP. The presence of the luciferase gene was confirmed in NR-40347 by PCR and by luciferase activity.
Note: The luciferase expressing plasmid was not integrated into the T. cruzi genome. It is recommended that luciferase activity be assayed monthly to confirm plasmid retention.
Each vial of NR-46429 contains approximately 0.5 mL of cells in cryopreservative [5% dimethylsulfoxide (DMSO)].
Note: The strain designation on the label is incorrect. The correct strain designation is Brazil (+luc). NR-46429 was deposited to BEI Resources as strain Y (+luc) and updated by the depositor to strain Brazil (+luc).
The epimastigote stage of Trypanosoma cruzi (T. cruzi), strain Brazil (+luc) was established from the trypomastigote form (BEI Resources NR-40347) through multiple passages in culture. The Brazil (+luc) strain is a luciferase-expressing transgenic clone derived from the Brazil strain, which was isolated from a human patient in Brazil.
The luciferase gene was introduced to the Brazil strain of T. cruzi via the pTREX-GFP plasmid where luc replaced GFP. The presence of the luciferase gene was confirmed in NR-40347 by PCR and by luciferase activity.
Note: The luciferase expressing plasmid was not integrated into the T. cruzi genome. It is recommended that luciferase activity be assayed monthly to confirm plasmid retention.
Each vial of NR-46429 contains approximately 0.5 mL of cells in cryopreservative [5% dimethylsulfoxide (DMSO)].
主要内容
此项目的每个订单数量限制为1.此商品每年可订购两次.通过此限制的订单将在发货前发送至NIAID进行批准.
注意:标签上的应变名称不正确.正确的应变名称是巴西(+ Luc ).将NR-46429沉积在BEI资源中,作为菌株Y(+ Luc,Luc ),并由存款仪更新到菌株巴西(+ Luc ).
通过培养的多个通道从胰透视形式(Bei Resources NR-40347)建立了触发瘤Cruzi(T.Cruzi),菌株巴西(+ Luc-/ I>)的映自夸(克鲁齐). .巴西(+ 1> luc rucl)菌株是衍生自巴西菌株的荧光素酶的转基因克隆,其从巴西的人患者中分离出来.
将荧光素酶基因引入 T的巴西菌株中. Cruzi通过ptrex-gfp质粒,其中 luc 更换了GFP.通过PCR和荧??光素酶活性在NR-40347中确认荧光素酶基因的存在.
注意:表达质粒的荧光素酶未整合到 T中. Cruzi 基因组.建议每月测定荧光素酶活性以确认质粒保留.
NR-46429的每个小瓶中含有约0.5ml冷冻渣的细胞[5%二甲基硫氧化物
(DMSO)].
厂牌介绍
BEI Resources 由美国国家过敏和传染病研究所 ( NIAID )成立,旨在为研究 A、B 和 C 类优先病原体、新兴传染病病原体、非病原微生物和其他相关微生物材料提供试剂、工具和信息到研究界。BEI Resources 获取、验证和生产科学家进行基础研究和开发改进的诊断测试、疫苗和疗法所需的试剂。通过将这些功能集中在 BEI Resources 中,可以监控科学界对这些材料的访问和使用,并确保试剂的质量控制。
除了为传染病界提供材料外,BEI Resources 还鼓励和支持研究人员和机构的材料存放。使用 BEI Resources存放材料对研究人员和研究社区有许多优势,包括安全存储、社区访问和分发;同时保护存款人的知识产权。只要有需要,BEI 资源库将作为研究人员的资源进行维护。您在 BEI Resources 的存款是一项有助于未来研究的长期投资。
BEI Resources 自 2003 年起由美国典型培养物保藏中心 (ATCC) 根据合同管理。2016 年 5 月, ATCC获得了一份为期七年的继续管理 BEI Resources的合同。合同范围已扩大到更全面的研究目录材料,包括由其他政府支持的研究项目存放的材料,将提供给生物防御和新兴传染病科学界。真菌、寄生虫、载体和其他相关材料已添加到现有的细菌、病毒和毒素试剂中,涵盖 NIAID A、B 和 C 类优先病原体和 NIAID 指定的新发传染病病原体和生物。
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