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产品图片
货号/SKU
NR-42868
货号/规格
EA
库存与交货期
3-8周
人民币价格
14000
试剂海关审批
A/B级风险物质只能直接使用人购买并持有实验室有效资质，其它询客服确认
国外采购
支持/部分限制一年内购买数量
厂牌
BEI Resources(ATCC)
产品基础信息
生物安全等级建议分类：美国、2
产品描述信息
NR-42868??Salmonella enterica subsp. enterica, 14028s (Serovar Typhimurium) Single-Gene Deletion Mutant Library, Plate SGD_033/034_Cm(Mutant Bacteria)|Salmonella enterica subsp. enterica|14028s (Serovar Typhimurium) Single-Gene Deletion Mutant Library, Plate SGD_033/034_Cm|-80°C or colder|M McClellandAcknowledgment for publications should read "The following reagent was obtained through BEI Resources, NIAID, NIH: Salmonella enterica subsp. enterica, Strain 14028s (Serovar Typhimurium) Single-Gene Deletion Mutant Library, Plate SGD_033/034_Cm, NR-42868."|Quantity limit per order for this item is 1. This item can be ordered twice a year. Orders over this limit will be sent to NIAID for approval before shipment.
The Salmonella enterica (S. enterica) subsp. enterica, strain 14028s (serovar Typhimurium) targeted single-gene deletion (SGD) mutant library contains a total of 3,773 individual genes deleted simultaneously across two collections of mutants differentiated by kanamycin or chloramphenicol resistance. The chloramphenicol-resistant mutant collection contains 3,376 mutants distributed among eleven 96-well plates, in which a single gene is replaced by a cassette conferring the chloramphenicol resistance gene, and includes 4 double mutants that contain both kanamycin and chloramphenicol cassettes. Deletions were confirmed by the depositor.
Genes were targeted for deletion by primers designed to preserve the first and last 30 bases of each deleted gene. Gene replacement followed a modified Lambda-Red technique, with an added T7 RNA polymerase promoter positioned in plasmid pCLF3 to generate a gene-specific transcript from the Salmonella genome directly downstream of each mutant.
Note: The strain designation on the plate, strain CDC 6516-60, is incorrect. The correct strain designation is strain 14028s. S. enterica subsp. enterica, strain 14028s was originally known as strain 14028. A variant of the original strain with a rough colony morphology was designated 14028r and the original smooth strain was renamed 14028s. Strain 14028 is a descendent of strain CDC 6516-60, which was isolated from pools of hearts and livers of 4-week-old chickens. The complete genome of S. enterica subsp. enterica, strain 14028s (GenBank: CP001363.1) and plasmid (GenBank: CP001362.1) sequences are available.
Each inoculated well of the 96-well plate contains approximately 50 ?L of culture in Luria Bertani (LB) broth containing 60 ?g/mL kanamycin supplemented with 10% glycerol.
Production in the 96-well format has increased risk of cross-contamination between adjacent wells. Individual clones should be purified (e.g. single colony isolation and purification using good microbiological practices) and sequence-verified prior to use. BEI Resources does not confirm or validate individual mutants provided by the contributor.
The Salmonella enterica (S. enterica) subsp. enterica, strain 14028s (serovar Typhimurium) targeted single-gene deletion (SGD) mutant library contains a total of 3,773 individual genes deleted simultaneously across two collections of mutants differentiated by kanamycin or chloramphenicol resistance. The chloramphenicol-resistant mutant collection contains 3,376 mutants distributed among eleven 96-well plates, in which a single gene is replaced by a cassette conferring the chloramphenicol resistance gene, and includes 4 double mutants that contain both kanamycin and chloramphenicol cassettes. Deletions were confirmed by the depositor.
Genes were targeted for deletion by primers designed to preserve the first and last 30 bases of each deleted gene. Gene replacement followed a modified Lambda-Red technique, with an added T7 RNA polymerase promoter positioned in plasmid pCLF3 to generate a gene-specific transcript from the Salmonella genome directly downstream of each mutant.
Note: The strain designation on the plate, strain CDC 6516-60, is incorrect. The correct strain designation is strain 14028s. S. enterica subsp. enterica, strain 14028s was originally known as strain 14028. A variant of the original strain with a rough colony morphology was designated 14028r and the original smooth strain was renamed 14028s. Strain 14028 is a descendent of strain CDC 6516-60, which was isolated from pools of hearts and livers of 4-week-old chickens. The complete genome of S. enterica subsp. enterica, strain 14028s (GenBank: CP001363.1) and plasmid (GenBank: CP001362.1) sequences are available.
Each inoculated well of the 96-well plate contains approximately 50 ?L of culture in Luria Bertani (LB) broth containing 60 ?g/mL kanamycin supplemented with 10% glycerol.
Production in the 96-well format has increased risk of cross-contamination between adjacent wells. Individual clones should be purified (e.g. single colony isolation and purification using good microbiological practices) and sequence-verified prior to use. BEI Resources does not confirm or validate individual mutants provided by the contributor.
主要内容
此项目的每个订单数量限制为1.此商品每年可订购两次.在此限制上订单将在发货前发送到NIAID批准. 沙门氏肠（S. enterica） subsp. 肠杆菌，菌株14028s（Serovar Typhimurium）靶向单基因缺失（SGD）突变文库含有总共3,773个单独的基因，横跨由卡那霉素或氯霉素抗性分化的两种突变体.抗氯霉素抗突变体收集含有3,376个突变体，其中在116孔板中分布，其中单个基因被赋予氯霉素抗性基因的盒代替，包括含有卡那霉素和氯霉素盒的4个双突变体.储存者证实了缺失. 基因旨在通过旨在保留每种缺失基因的第一和最后30个基因的引物删除.基因更换遵循改性的λ-红色技术，其中添加的T7 RNA聚合酶启动子定位于质粒 pclf3 从每个突变体的下游产生来自沙门氏菌的基因组的基因特异性转录物.正确的应变指定是应变14028s. s. enterica 子公司. 肠溶，菌株14028s最初称为菌株14028.用粗糙菌落形态的原始菌株的变型被指定为14028r，并将原始的光滑菌株重新命名为14028s.菌株14028是菌株CDC 6516-60的后代，其与4周龄鸡的心脏和肝脏池中分离出来. s的完整基因组. enterica 子公司. entenica ，应变14028s（genbank： CP001363.1 ）和质粒（Genbank： cp001362.1 ）序列可用.每个接种井在96孔板中含有大约50μl的含有约50μl的培养物在含有60μg/ ml卡那霉素的Luria Bertani（LB）肉汤中，其补充有10％甘油. 96的生产 - 相邻井之间的交叉污染风险增加了.应纯化各个克隆（例如，使用良好的微生物实践的单菌落分离和纯化）并在使用前进行序列验证. Bei资源不确认或验证贡献者提供的个体突变体.
厂牌介绍
BEI Resources 由美国国家过敏和传染病研究所 ( NIAID )成立，旨在为研究 A、B 和 C 类优先病原体、新兴传染病病原体、非病原微生物和其他相关微生物材料提供试剂、工具和信息到研究界。BEI Resources 获取、验证和生产科学家进行基础研究和开发改进的诊断测试、疫苗和疗法所需的试剂。通过将这些功能集中在 BEI Resources 中，可以监控科学界对这些材料的访问和使用，并确保试剂的质量控制。
除了为传染病界提供材料外，BEI Resources 还鼓励和支持研究人员和机构的材料存放。使用 BEI Resources存放材料对研究人员和研究社区有许多优势，包括安全存储、社区访问和分发；同时保护存款人的知识产权。只要有需要，BEI 资源库将作为研究人员的资源进行维护。您在 BEI Resources 的存款是一项有助于未来研究的长期投资。
BEI Resources 自 2003 年起由美国典型培养物保藏中心 (ATCC) 根据合同管理。2016 年 5 月， ATCC获得了一份为期七年的继续管理 BEI Resources的合同。合同范围已扩大到更全面的研究目录材料，包括由其他政府支持的研究项目存放的材料，将提供给生物防御和新兴传染病科学界。真菌、寄生虫、载体和其他相关材料已添加到现有的细菌、病毒和毒素试剂中，涵盖 NIAID A、B 和 C 类优先病原体和 NIAID 指定的新发传染病病原体和生物。
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