产品图片

货号/SKU
NR-36206
货号/规格
EA
库存与交货期
3-8周
人民币价格
14000
试剂海关审批
A/B级风险物质只能直接使用人购买并持有实验室有效资质,其它询客服确认
国外采购
支持/部分限制一年内购买数量
厂牌
BEI Resources(ATCC)
产品基础信息
生物安全等级建议分类:美国、1
产品描述信息
NR-36206??Cavia porcellus, Guinea Pig Expression Clone CD8β/pcDNA3.1 Hygro(+)(Clones)|Cavia porcellus|Guinea Pig Expression Clone CD8β/pcDNA3.1 Hygro(+)|-20°C or colder|RG RankAcknowledgment for publications should read "The following reagent was obtained through BEI Resources, NIAID, NIH: Guinea Pig Expression Clone CD8β/pcDNA3.1 Hygro(+), NR-36206."|Quantity limit per order for this item is 1. This item can be ordered twice a year. Orders over this limit will be sent to NIAID for approval before shipment.
NR-36206 is a full-length expression clone containing the CD8β gene (GenBank: AY303774.1) from guinea pig (Cavia porcellus). The CD8β gene was cloned into pcDNA3.1/hygro(+) via BamHI and EcoRV insertion sites, transformed into MAX Efficiency?Escherichia coli (E. coli) DH5α? competent cells (Invitrogen?), and extracted using a QIAGEN? Plasmid Mega Kit.
Note: Sequencing results for NR-36206 revealed that most regions of the vector match the depositor's information, including the ampicillin resistance gene, the hygromycin resistance gene under control of the SV40 promoter, polyadenylation signals, and the pUC and f1 origins of replication. However, the native cytomegalovirus (CMV) promoter has been replaced by a ubiquitin (UbC) promoter upstream of CD8β; this replacement also resulted in the loss of the T7 promoter. The hygromycin resistance gene contains an in-frame 21-base-pair deletion which removes seven amino acids near the C-terminal end of the protein (not near the active site). The resulting size of the plasmid is 6.540 kilobases. The plasmid map and the complete plasmid sequence are provided on the Certificate of Analysis for NR-36206.
Each vial of NR-36206 contains approximately 1 ?g of plasmid DNA in TE buffer.
Note: The vial labels for NR-36206 incorrectly state that the plasmid DNA is provided as an E. coli glycerol stock. NR-36206 is purified plasmid DNA that was extracted from bacteria prior to vialing.
NR-36206 is a full-length expression clone containing the CD8β gene (GenBank: AY303774.1) from guinea pig (Cavia porcellus). The CD8β gene was cloned into pcDNA3.1/hygro(+) via BamHI and EcoRV insertion sites, transformed into MAX Efficiency?Escherichia coli (E. coli) DH5α? competent cells (Invitrogen?), and extracted using a QIAGEN? Plasmid Mega Kit.
Note: Sequencing results for NR-36206 revealed that most regions of the vector match the depositor's information, including the ampicillin resistance gene, the hygromycin resistance gene under control of the SV40 promoter, polyadenylation signals, and the pUC and f1 origins of replication. However, the native cytomegalovirus (CMV) promoter has been replaced by a ubiquitin (UbC) promoter upstream of CD8β; this replacement also resulted in the loss of the T7 promoter. The hygromycin resistance gene contains an in-frame 21-base-pair deletion which removes seven amino acids near the C-terminal end of the protein (not near the active site). The resulting size of the plasmid is 6.540 kilobases. The plasmid map and the complete plasmid sequence are provided on the Certificate of Analysis for NR-36206.
Each vial of NR-36206 contains approximately 1 ?g of plasmid DNA in TE buffer.
Note: The vial labels for NR-36206 incorrectly state that the plasmid DNA is provided as an E. coli glycerol stock. NR-36206 is purified plasmid DNA that was extracted from bacteria prior to vialing.
主要内容
此项目的每个订单数量限制为1.此商品每年可订购两次.此限制的订单将在发货前发送到NIAID进行批准. NR-36206是包含CD8β基因(Genbank:)来自豚鼠(豚鼠porcellus ).将CD8β基因克隆到PCDNA3.1 / hygro(+)中通过 Bam HI和 eCO / i> RV插入位点,转化为最大效率? 大肠杆菌(大肠杆菌)(大肠杆菌)DH5α?珍珠电池(Invitrogen?),并使用Qiagen ?质粒套件提取. 注意:NR-36206的测序结果表明,向量的大多数区域匹配沉积仪的信息,包括氨苄青霉素抗性基因,在SV40启动子,聚腺苷酸化信号和PUC和PUC和F1的控制下匹配潮霉素抗性基因复制.然而,本地巨细胞病毒(CMV)启动子已被CD8β上游的泛素(UBC)启动子取代;这种替代物也导致T7启动子的丧失.潮霉素抵抗基因含有框架的21-碱基对缺失,其除去蛋白质的C末端附近的七个氨基酸(不附近的活性位点).得到的质粒尺寸为6.540千碱基.对NR-36206的分析证书提供了质粒图和完全质粒序列. 每个小瓶的NR-36206含有约1μg缓冲液中的质粒DNA. 注意:NR-36206的小瓶标签不正确地表示质粒DNA作为 e提供. Coli 甘油股票. NR-36206是纯化的质粒DNA,在瓶中在细菌中提取.
厂牌介绍
BEI Resources 由美国国家过敏和传染病研究所 ( NIAID )成立,旨在为研究 A、B 和 C 类优先病原体、新兴传染病病原体、非病原微生物和其他相关微生物材料提供试剂、工具和信息到研究界。BEI Resources 获取、验证和生产科学家进行基础研究和开发改进的诊断测试、疫苗和疗法所需的试剂。通过将这些功能集中在 BEI Resources 中,可以监控科学界对这些材料的访问和使用,并确保试剂的质量控制。
除了为传染病界提供材料外,BEI Resources 还鼓励和支持研究人员和机构的材料存放。使用 BEI Resources存放材料对研究人员和研究社区有许多优势,包括安全存储、社区访问和分发;同时保护存款人的知识产权。只要有需要,BEI 资源库将作为研究人员的资源进行维护。您在 BEI Resources 的存款是一项有助于未来研究的长期投资。
BEI Resources 自 2003 年起由美国典型培养物保藏中心 (ATCC) 根据合同管理。2016 年 5 月, ATCC获得了一份为期七年的继续管理 BEI Resources的合同。合同范围已扩大到更全面的研究目录材料,包括由其他政府支持的研究项目存放的材料,将提供给生物防御和新兴传染病科学界。真菌、寄生虫、载体和其他相关材料已添加到现有的细菌、病毒和毒素试剂中,涵盖 NIAID A、B 和 C 类优先病原体和 NIAID 指定的新发传染病病原体和生物。
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