产品图片
货号/SKU
NR-15792
货号/规格
EA
库存与交货期
3-8周
人民币价格
14000
试剂海关审批
A/B级风险物质只能直接使用人购买并持有实验室有效资质,其它询客服确认
国外采购
支持/部分限制一年内购买数量
厂牌
BEI Resources(ATCC)
产品基础信息
生物安全等级建议分类:美国、3
产品描述信息
NR-15792??Mycobacterium tuberculosis, CDC1551, Transposon Mutant Knock-Out Pool 20(Mutant Bacteria)|Mycobacterium tuberculosis|CDC1551, Transposon Mutant Knock-Out Pool 20|-60°C or colder|WR BishaiAcknowledgment for publications should read "The following reagent was obtained through BEI Resources, NIAID, NIH: Mycobacterium tuberculosis, Strain CDC1551, Transposon Mutant Knock-Out Pool 20, NR-15792."|Quantity limit per order for this item is 1. This item can be ordered twice a year. Orders over this limit will be sent to NIAID for approval before shipment.
In 2002, TARGET (Tuberculosis Animal Research and Gene Evaluation Taskforce) was formed to enable the modeling of human tuberculosis in multiple animal species using defined protocols and testing defined mutants of M. tuberculosis. In addition to animal modeling activities, a library of intragenic transposon mutants has been created and characterized.
There are 20 transposon mutant knock-out pools available from BEI Resources (NR-15773 to NR-15792) that are companion products to the DeADMAn DNA Microarray (available from BEI Resources as NR-18958). The DeADMAn DNA Microarray is used for identification of genes essential for the survival of a stress condition in an in vivo model system infection.
M. tuberculosis, strain CDC1551 transposon mutant knock-out pool 20 is reported to be a mixture of 20 genetically defined M. tuberculosis transposon mutants. Some of the transposon mutants in knock-out pool 20 are available individually (see Pool 20 components below).
Each vial contains approximately 1 mL of bacterial culture in Middlebrook 7H9 broth with OADC enrichment containing 100 ?g/mL cycloheximide and 20 ?g/mL kanamycin.
Components:
In 2002, TARGET (Tuberculosis Animal Research and Gene Evaluation Taskforce) was formed to enable the modeling of human tuberculosis in multiple animal species using defined protocols and testing defined mutants of M. tuberculosis. In addition to animal modeling activities, a library of intragenic transposon mutants has been created and characterized.
There are 20 transposon mutant knock-out pools available from BEI Resources (NR-15773 to NR-15792) that are companion products to the DeADMAn DNA Microarray (available from BEI Resources as NR-18958). The DeADMAn DNA Microarray is used for identification of genes essential for the survival of a stress condition in an in vivo model system infection.
M. tuberculosis, strain CDC1551 transposon mutant knock-out pool 20 is reported to be a mixture of 20 genetically defined M. tuberculosis transposon mutants. Some of the transposon mutants in knock-out pool 20 are available individually (see Pool 20 components below).
Each vial contains approximately 1 mL of bacterial culture in Middlebrook 7H9 broth with OADC enrichment containing 100 ?g/mL cycloheximide and 20 ?g/mL kanamycin.
Components:
Description of Transposon Knock-Out Mutant | Strain CDC1551 Gene | Strain H37Rv Gene1 | BEI Resources Product Number2 |
---|---|---|---|
PPE FAMILY PROTEIN | MT2840 | Rv2770c | NA |
HYPOTHETICAL PROTEIN | MT1537 | Rv* | NA |
PROBABLE LIPOPROTEIN LPPT | MT1848 | Rv1799 | NA |
PROBABLE NITRATE REDUCTASE NARX | MT1778 | Rv1736c | NA |
TRANSCRIPTIONAL REGULATOR | MT1712 | Rv1674c | NR-14745 |
POSSIBLE TRANSCRIPTIONAL REGULATORY PROTEIN | MT2319 | Rv2258c | NR-17995 |
CONSERVED HYPOTHETICAL PROTEIN | MT1003.1 | Rv0976c | NR-15125 |
VIRULENCE FACTOR | MT3599 | Rv3495c | NR-13633 |
PROBABLE SHORT CHAIN ALCOHOL DEHYDROGENASE/REDUCTASE | MT3143 | Rv3057c | NA |
PROBABLE EPOXIDE HYDROLASE | MT1988 | Rv1938 | NR-18002 |
TRANSPORT PROTEIN | MT0951 | Rv0924c | NR-13634 |
PROBABLE PRECORRIN-6Y METHYLTRANSFERASE CobL | MT2132 | Rv2072c | NA |
PROBABLE TRANSMEMBRANE PROTEIN | MT1272 | Rv1234 | NA |
POSSIBLE LIPASE LIPU | MT1106 | Rv1076 | NA |
PROBABLE ATP-DEPENDENT HELICASE DING | MT1371 | Rv1329c | NA |
CONSERVED HYPOTHETICAL PROTEIN | MT2701 | Rv2626c | NA |
PROBABLE FATTY-ACID-CoA LIGASE FADD30 | MT0417 | Rv0404 | NA |
PROBABLE MEMBRANE PROTEIN | MT2152 | Rv2091c | NA |
CONSERVED HYPOTHETICAL PROTEIN | MT3980 | Rv3866 | NR-15127 |
POSSIBLE PROTEASE IV SPPA | MT0749 | Rv0724 | NA |
1Rv* - In some cases there is no M. tuberculosis, strain H37Rv homologue to the M. tuberculosis, strain CDC1551 gene | |||
2NA - Individual transposon mutant not available from BEI Resources but may be available from TARGET |
主要内容
此项目的每个订单数量限制为1.此商品每年可订购两次.通过此限制的订单将在发货前发送到NIAID进行批准. target (结核病动物研究和基因评估任务性)形成为使用定义的方案和测试的定义的突变体来实现多种动物物种中的人结核病建模.结核病.除了动物建模活动外,还创建和表征了一种腺体转座子突变体. 有来自BEI资源(NR-15773至NR-15792)的20名转座突变体敲除池产品到Deadman DNA微阵列(可从BEI资源获得NR-18958).死亡DNA微阵列用于鉴定在体内体内系统感染中的胁迫条件的生存基因所必需的基因. m.结核病,菌株CDC1551转座子突变突变池20被据报道为20种遗传定义的 m的混合物.结核病转座突变体.敲除池20中的一些转座突变体可单独使用(参见下面的池20个组分). 每个小瓶在含有100μg/ ml环己酰胺的OADC富集中含有约1ml的细菌培养物和20μg/ ml卡那霉素. 组件: 描述转座子敲除突变体 菌株 cdc1551基因 菌株H37RV 基因 1 bei资源 产品编号 2 ppe家族蛋白 mt2840 rv2770c na 假设蛋白 mt1537 RV * Na 可能脂蛋白LPPT Mt1848 Rv1799 Na 可能硝酸还原酶Narx Mt1778 RV1736C Na 转录调节器 MT1712 RV1674C nr-14745 可能的转录调节蛋白 mt2319 rv2258c nr-17995 保守假设蛋白 mt1003.1 rv0976c nr-15125 毒力因子 mt3599 rv3495c NR-13633 可能的短链醇脱氢酶/还原酶 MT3143 RV3057C Na 可能环氧化物水解酶 Mt1988 RV1938 nr-18002 传输蛋白 mt0951 rv0924c nr-13634 可能的预rorrin-6y甲基转移酶cobl mt2132 rv2072c na 可能跨膜蛋白 mt1272 RV1234 Na 可能脂肪酶Lipu MT1106 RV1076 na 可能的ATP依赖性螺旋酶Ding Mt1371 RV1329C 保守假设蛋白 Mt2701 RV2626C Na 可能脂肪酸 - COA连接酶FADD30 MT0417 RV0404 Na 可能膜蛋白 MT2152 RV2091C Na 保守假设蛋白 mt3980 rv3866 nr-15127 可能的蛋白酶IV sppa mt0749 rv0724 na 1 rv * - 在某些情况下没有 m.结核病,菌株H37RV同源物至 m.结核病,菌株CDC1551基因 2 na - 从BEI资源不提供的单个转座子突变体,但可以从 target
厂牌介绍
BEI Resources 由美国国家过敏和传染病研究所 ( NIAID )成立,旨在为研究 A、B 和 C 类优先病原体、新兴传染病病原体、非病原微生物和其他相关微生物材料提供试剂、工具和信息到研究界。BEI Resources 获取、验证和生产科学家进行基础研究和开发改进的诊断测试、疫苗和疗法所需的试剂。通过将这些功能集中在 BEI Resources 中,可以监控科学界对这些材料的访问和使用,并确保试剂的质量控制。
除了为传染病界提供材料外,BEI Resources 还鼓励和支持研究人员和机构的材料存放。使用 BEI Resources存放材料对研究人员和研究社区有许多优势,包括安全存储、社区访问和分发;同时保护存款人的知识产权。只要有需要,BEI 资源库将作为研究人员的资源进行维护。您在 BEI Resources 的存款是一项有助于未来研究的长期投资。
BEI Resources 自 2003 年起由美国典型培养物保藏中心 (ATCC) 根据合同管理。2016 年 5 月, ATCC获得了一份为期七年的继续管理 BEI Resources的合同。合同范围已扩大到更全面的研究目录材料,包括由其他政府支持的研究项目存放的材料,将提供给生物防御和新兴传染病科学界。真菌、寄生虫、载体和其他相关材料已添加到现有的细菌、病毒和毒素试剂中,涵盖 NIAID A、B 和 C 类优先病原体和 NIAID 指定的新发传染病病原体和生物。
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