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产品图片
货号/SKU
NR-14851
货号/规格
EA
库存与交货期
3-8周
人民币价格
14000
试剂海关审批
A/B级风险物质只能直接使用人购买并持有实验室有效资质，其它询客服确认
国外采购
支持/部分限制一年内购买数量
厂牌
BEI Resources(ATCC)
产品基础信息
生物安全等级建议分类：美国、1
产品描述信息
NR-14851??Mycobacterium tuberculosis, H37Rv, Purified Mycolylarabinogalactan-Peptidoglycan (mAGP)(Antigen Preparations)|Mycobacterium tuberculosis|H37Rv, Purified Mycolylarabinogalactan-Peptidoglycan (mAGP)|Room temperature (dry atmosphere)|BEI Resources or NIH - TB Vaccine Testing and Research Materials ContractAcknowledgment for publications should read "The following reagent was obtained through BEI Resources, NIAID, NIH: Mycobacterium tuberculosis, Strain H37Rv, Purified Mycolylarabinogalactan-Petidoglycan (mAGP), NR-14851."|Quantity limit per order for this item is 1. This item can be ordered twice a year. Orders over this limit will be sent to NIAID for approval before shipment.
NR-14851 is a preparation of the mycolylarabinogalactan-peptidoglycan (mAGP) complex of the Mycobacterium tuberculosis (M. tuberculosis), strain H37Rv cell wall core.
The culture was grown to late-log phase in glycerol-alanine-salts medium, washed with PBS pH 7.4 and inactivated by gamma irradiation. The bacilli were suspended at a concentration of 2 g/mL in PBS containing 8 mM EDTA, DNase, RNase and a proteinase inhibitor tablet, and broken in a French Press pressure cell at 4°C. Unbroken cells were removed by low speed (3,000 × g) centrifugation. The cell wall was isolated by centrifugation at 27,000 × g for one hour, the pellet suspended in PBS with 2% SDS and the cell wall associated proteins and lipoglycans were extracted at room temperature. Residual contaminating proteins were removed from the cell wall by further SDS extraction and proteinase-K treatment at 55°C, followed by several extractions with boiling SDS. The resulting mAGP was washed several times with water, and residual SDS was removed by extraction with large volumes of acetone.
Each vial contains approximately 5 mg of dried, purified mAGP from M. tuberculosis, strain H37Rv.
Note: mAGP is soluble in acetone. DMSO or water can also be used depending on the downstream application. mAGP is poorly soluble and sonication is generally required to achieve a uniform suspension.
NR-14851 is a preparation of the mycolylarabinogalactan-peptidoglycan (mAGP) complex of the Mycobacterium tuberculosis (M. tuberculosis), strain H37Rv cell wall core.
The culture was grown to late-log phase in glycerol-alanine-salts medium, washed with PBS pH 7.4 and inactivated by gamma irradiation. The bacilli were suspended at a concentration of 2 g/mL in PBS containing 8 mM EDTA, DNase, RNase and a proteinase inhibitor tablet, and broken in a French Press pressure cell at 4°C. Unbroken cells were removed by low speed (3,000 × g) centrifugation. The cell wall was isolated by centrifugation at 27,000 × g for one hour, the pellet suspended in PBS with 2% SDS and the cell wall associated proteins and lipoglycans were extracted at room temperature. Residual contaminating proteins were removed from the cell wall by further SDS extraction and proteinase-K treatment at 55°C, followed by several extractions with boiling SDS. The resulting mAGP was washed several times with water, and residual SDS was removed by extraction with large volumes of acetone.
Each vial contains approximately 5 mg of dried, purified mAGP from M. tuberculosis, strain H37Rv.
Note: mAGP is soluble in acetone. DMSO or water can also be used depending on the downstream application. mAGP is poorly soluble and sonication is generally required to achieve a uniform suspension.
主要内容
此项目的每个订单数量限制为1.此商品每年可订购两次.此限制的订单将在发货前发送到NIAID批准.
NR-14851是一种制备硫代吡喃 - 肽 - 肽聚糖（MagP）复合物的结核分枝杆菌（Cuberculosis） - 菌株H37RV细胞壁芯.
将培养物生长至甘油 - 丙氨酸 - 盐培养基中的后对数相，用PBS pH 7.4洗涤并通过γ辐射灭活.将溶液悬浮在含有8mM EDTA，DNA酶，RNase和蛋白酶抑制剂片中的PBS中的2g / ml的浓度，并在4℃下在法国压力下破裂.通过低速（3,000×g）离心除去不间断的细胞.通过以27,000×g离心1小时，将细胞壁分离1小时，在室温下悬浮在PBS中悬浮在PBS中的沉淀和细胞壁相关蛋白质和脂肪组.通过进一步的SDS萃取和在55℃下处理从细胞壁中除去残留的污染蛋白，然后用沸点SDS进行几种萃取.将所得MAG用水洗涤数次，通过用大量的丙酮提取除去残留的SDS.
每个小瓶含有约5mg干燥的，纯化的Magp从 m.结核病，菌株H37RV.
注意：MAG溶于丙酮.根据下游应用，也可以使用DMSO或水. MAGP易溶，并且通常需要超声处理来实现均匀的悬浮液.
厂牌介绍
BEI Resources 由美国国家过敏和传染病研究所 ( NIAID )成立，旨在为研究 A、B 和 C 类优先病原体、新兴传染病病原体、非病原微生物和其他相关微生物材料提供试剂、工具和信息到研究界。BEI Resources 获取、验证和生产科学家进行基础研究和开发改进的诊断测试、疫苗和疗法所需的试剂。通过将这些功能集中在 BEI Resources 中，可以监控科学界对这些材料的访问和使用，并确保试剂的质量控制。
除了为传染病界提供材料外，BEI Resources 还鼓励和支持研究人员和机构的材料存放。使用 BEI Resources存放材料对研究人员和研究社区有许多优势，包括安全存储、社区访问和分发；同时保护存款人的知识产权。只要有需要，BEI 资源库将作为研究人员的资源进行维护。您在 BEI Resources 的存款是一项有助于未来研究的长期投资。
BEI Resources 自 2003 年起由美国典型培养物保藏中心 (ATCC) 根据合同管理。2016 年 5 月， ATCC获得了一份为期七年的继续管理 BEI Resources的合同。合同范围已扩大到更全面的研究目录材料，包括由其他政府支持的研究项目存放的材料，将提供给生物防御和新兴传染病科学界。真菌、寄生虫、载体和其他相关材料已添加到现有的细菌、病毒和毒素试剂中，涵盖 NIAID A、B 和 C 类优先病原体和 NIAID 指定的新发传染病病原体和生物。
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