产品图片
货号/SKU
NR-12188
货号/规格
EA
库存与交货期
3-8周
人民币价格
14000
试剂海关审批
A/B级风险物质只能直接使用人购买并持有实验室有效资质,其它询客服确认
国外采购
支持/部分限制一年内购买数量
厂牌
BEI Resources(ATCC)
产品基础信息
生物安全等级建议分类:美国、1
产品描述信息
NR-12188?? Monoclonal Anti-Bacillus anthracis Lethal Factor, Clone LF-9A11 (produced in vitro)(Monoclonal Antibodies)|Bacillus anthracis|Monoclonal Anti-Bacillus anthracis Lethal Factor, Clone LF-9A11 (produced in vitro)|-20°C or colder|B HaynesAcknowledgment for publications should read "The following reagent was obtained through BEI Resources, NIAID, NIH: Monoclonal Anti-Bacillus anthracis Lethal Factor, Clone LF-9A11 (produced in vitro), NR-12188."|Quantity limit per order for this item is 1. This item can be ordered twice a year. Orders over this limit will be sent to NIAID for approval.
Antibody Class: IgG1κ
Monoclonal antibody prepared against recombinant lethal factor (rLF) from Bacillus anthracis (B. anthracis) was purified from hybridoma clone LF-9A11 supernatant by protein G affinity chromatography. The B cell hybridoma was generated by the fusion of P3X63-Ag8 BALB/c mouse myeloma cells with splenocytes from female C57BL/6 x BALB/c F1 mice immunized intranasally with purified rLF.
Note: The P3X63-Ag8 myeloma cell line secretes the MOPC21 myeloma protein, a mouse IgG1κ antibody of unknown specificity. Thus, NR-12188 may contain both MOPC21 protein and B. anthracis LF-specific antibody of the IgG1κ isotype, as well as inactive hybrid immunoglobulin molecules.
Each vial of NR-12188 contains approximately 100 ?L of purified monoclonal antibody in PBS.
NR-12188 is released without confirmation of functional activity. Clone LF-9A11 antibody is reported to bind B. anthracis LF in ELISA and surface plasmon resonance assays. The antibody is also reported to neutralize anthrax lethal toxin in cell-based assays, and passive transfer provided statistically significant protection against morbidity in a mouse lethal toxin challenge model.
Antibody Class: IgG1κ
Monoclonal antibody prepared against recombinant lethal factor (rLF) from Bacillus anthracis (B. anthracis) was purified from hybridoma clone LF-9A11 supernatant by protein G affinity chromatography. The B cell hybridoma was generated by the fusion of P3X63-Ag8 BALB/c mouse myeloma cells with splenocytes from female C57BL/6 x BALB/c F1 mice immunized intranasally with purified rLF.
Note: The P3X63-Ag8 myeloma cell line secretes the MOPC21 myeloma protein, a mouse IgG1κ antibody of unknown specificity. Thus, NR-12188 may contain both MOPC21 protein and B. anthracis LF-specific antibody of the IgG1κ isotype, as well as inactive hybrid immunoglobulin molecules.
Each vial of NR-12188 contains approximately 100 ?L of purified monoclonal antibody in PBS.
NR-12188 is released without confirmation of functional activity. Clone LF-9A11 antibody is reported to bind B. anthracis LF in ELISA and surface plasmon resonance assays. The antibody is also reported to neutralize anthrax lethal toxin in cell-based assays, and passive transfer provided statistically significant protection against morbidity in a mouse lethal toxin challenge model.
主要内容
此项目的每个订单数量限制为1.此项目每年可订购两次.下列限制的订单将被送到NIAID批准. 抗体类:IgG1κ单克隆抗体从杆菌(B.炭疽)的重组致死因子(RLF)制备通过蛋白G亲和色谱法从杂交瘤克隆LF-9a11上清液中纯化“. B细胞杂交瘤由P3X63-AG8 BALB / C小鼠骨髓瘤细胞的融合产生,含有纯化的RLF免疫的雌性C57BL / 6 X BALB / C F1小鼠的脾细胞. 注意:P3X63-AG8骨髓瘤细胞系分泌MOPC21骨髓瘤蛋白,一种未知特异性的小鼠IgG1κ抗体.因此,NR-12188可以含有MOPC21蛋白和 B. IgG1κ同种型的炭疽生特异性抗体,以及无活性的杂交免疫球蛋白分子. 每个小瓶的NR-12188在PBS中含有约100μl纯化的单克隆抗体. > NR-12188未经确认功能活性释放.据报道克隆LF-9A11抗体结合 B.炭疽和表面等离子体共振测定中的炭疽感.还报道抗体中和在基于细胞的测定中的炭疽致死毒素中和,并且被动转移提供了统计学上的致死毒素攻击模型中的发病率的显着保护.
厂牌介绍
BEI Resources 由美国国家过敏和传染病研究所 ( NIAID )成立,旨在为研究 A、B 和 C 类优先病原体、新兴传染病病原体、非病原微生物和其他相关微生物材料提供试剂、工具和信息到研究界。BEI Resources 获取、验证和生产科学家进行基础研究和开发改进的诊断测试、疫苗和疗法所需的试剂。通过将这些功能集中在 BEI Resources 中,可以监控科学界对这些材料的访问和使用,并确保试剂的质量控制。
除了为传染病界提供材料外,BEI Resources 还鼓励和支持研究人员和机构的材料存放。使用 BEI Resources存放材料对研究人员和研究社区有许多优势,包括安全存储、社区访问和分发;同时保护存款人的知识产权。只要有需要,BEI 资源库将作为研究人员的资源进行维护。您在 BEI Resources 的存款是一项有助于未来研究的长期投资。
BEI Resources 自 2003 年起由美国典型培养物保藏中心 (ATCC) 根据合同管理。2016 年 5 月, ATCC获得了一份为期七年的继续管理 BEI Resources的合同。合同范围已扩大到更全面的研究目录材料,包括由其他政府支持的研究项目存放的材料,将提供给生物防御和新兴传染病科学界。真菌、寄生虫、载体和其他相关材料已添加到现有的细菌、病毒和毒素试剂中,涵盖 NIAID A、B 和 C 类优先病原体和 NIAID 指定的新发传染病病原体和生物。
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