产品图片
货号/SKU
MRA-458
货号/规格
EA
库存与交货期
3-8周
人民币价格
14000
试剂海关审批
A/B级风险物质只能直接使用人购买并持有实验室有效资质，其它询客服确认
国外采购
支持/部分限制一年内购买数量
厂牌
BEI Resources(ATCC)
产品基础信息
生物安全等级建议分类：美国、1
产品描述信息
MRA-458??Plasmodium falciparum, pfGN-FTA Malaria Expression Vector(Plasmid/Vectors)|Plasmodium falciparum|pfGN-FTA Malaria Expression Vector|-20°C or colder|CV PloweAcknowledgment for publications should read "The following reagent was obtained through BEI Resources, NIAID, NIH: pfGN-FTA Malaria Expression Vector, MRA-458, contributed by Christopher V. Plowe."|Quantity limit per order for this item is 1. This item can be ordered twice a year. Orders over this limit will be sent to NIAID for approval before shipment.
This reagent was authenticated by the contributor. BEI Resources has not confirmed or validated this material.
pfGN-FTA is a malaria expression vector with a geneticin/kanamycin-selectable marker for growth in yeast and bacteria, respectively, while episomal selection in malaria is governed by a fused gfpm2/neo under control of malaria regulatory sequences. An antifolate-resistant dhfrts allele lacking a start codon and promoter allows for transfected antifolate-sensitive parasites to be selected for allelic replacement at the dhfrts locus under pyrimethamine pressure. The transfected parasites express the fused green fluorescent protein (GFP) for fluorescence-based analyses. This vector is useful for (1) analysis of unique dihydrofolate reductase (DHFR) mutants in malaria by yeast gap-repair of alleles into pfGN-FTA and transfection and allelic replacement in malaria, (2) stable integration of GFP into parasite strains at defined locus, and (3) locus-specific incorporation of heterologous gene of interest (replacing gfp-neo fusion) into malaria genome.
Each vial of MRA-458 contains plasmid DNA in TE buffer at a concentration of 1 ?g/?L.
Triglia, T., et al. "Allelic Exchange at the Endogenous Genomic Locus in Plasmodium falciparum Proves the Role of Dihydropteroate Synthase in Sulfadoxine-Resistant Malaria." EMBO J. 17 (1998): 3807-3815. PubMed: 9669998.
VanWye, J. D. and K. Haldar. "Expression of Green Fluorescent Protein in Plasmodium falciparum." Mol. Biochem. Parasitol. 87 (1997): 225-229. PubMed: 9247934.
Crabb, B. S., et al. "Stable Transgene Expression in Plasmodium falciparum." Mol. Biochem. Parasitol. 90 (1997): 131-144. PubMed: 9497038.
Mamoun, C. B., et al. "A Set of Independent Selectable Markers for Transfection of the Human Malaria Parasite Plasmodium falciparum." Proc. Natl. Acad. Sci. USA 96 (1999): 8716-8720. PubMed: 10411941.
This reagent was authenticated by the contributor. BEI Resources has not confirmed or validated this material.
pfGN-FTA is a malaria expression vector with a geneticin/kanamycin-selectable marker for growth in yeast and bacteria, respectively, while episomal selection in malaria is governed by a fused gfpm2/neo under control of malaria regulatory sequences. An antifolate-resistant dhfrts allele lacking a start codon and promoter allows for transfected antifolate-sensitive parasites to be selected for allelic replacement at the dhfrts locus under pyrimethamine pressure. The transfected parasites express the fused green fluorescent protein (GFP) for fluorescence-based analyses. This vector is useful for (1) analysis of unique dihydrofolate reductase (DHFR) mutants in malaria by yeast gap-repair of alleles into pfGN-FTA and transfection and allelic replacement in malaria, (2) stable integration of GFP into parasite strains at defined locus, and (3) locus-specific incorporation of heterologous gene of interest (replacing gfp-neo fusion) into malaria genome.
Each vial of MRA-458 contains plasmid DNA in TE buffer at a concentration of 1 ?g/?L.
Triglia, T., et al. "Allelic Exchange at the Endogenous Genomic Locus in Plasmodium falciparum Proves the Role of Dihydropteroate Synthase in Sulfadoxine-Resistant Malaria." EMBO J. 17 (1998): 3807-3815. PubMed: 9669998.
VanWye, J. D. and K. Haldar. "Expression of Green Fluorescent Protein in Plasmodium falciparum." Mol. Biochem. Parasitol. 87 (1997): 225-229. PubMed: 9247934.
Crabb, B. S., et al. "Stable Transgene Expression in Plasmodium falciparum." Mol. Biochem. Parasitol. 90 (1997): 131-144. PubMed: 9497038.
Mamoun, C. B., et al. "A Set of Independent Selectable Markers for Transfection of the Human Malaria Parasite Plasmodium falciparum." Proc. Natl. Acad. Sci. USA 96 (1999): 8716-8720. PubMed: 10411941.
主要内容
此项目的每个订单数量限制为1.此商品每年可订购两次.通过此限制的订单将在发货前发送至NIAID进行批准.
该试剂由贡献者认证. Bei资源尚未确认或验证了这种材料. Pfgn-FTA分别是一种疟疾表达载体，其分别是酵母和细菌生长的遗传蛋白/卡那霉素可选择的标记，而疟疾中的eCisomal选择受到疟疾调节序列的控制下的融合GFPM2 / Neo的管辖.缺乏起始密码子和启动子的抗性抗性 DHFRTS等位基因允许转染的抗灰酸敏感寄生虫，以在吡米甲胺压力下在 DHFRTS 基因座处选择等位基因替代品.转染的寄生虫表达了融合的绿色荧光蛋白（GFP），用于荧光基分析.该载体可用于（1）通过酵母间隙 - 在PFGN-FTA中的等位基因的修复和疟疾转染和等位基因替代的疟疾中独特的二氢醇还原酶（DHFR）突变体的分析是有用的，（2）稳定地将GFP与定义寄生虫菌株基因座，和（3）轨迹特异性掺入感兴趣的异源基因（将GFP-Neo融合融合）变为疟疾基因组.
MRA-458的每个小瓶含有Te缓冲液中的质粒DNA，浓度为1μg/μl.
Triglia，T.等人. “在疟原虫的内源性基因组轨迹处的等位基因交换是证明了二氢化盐合酶在磺基肟抗性疟疾中的作用.” Embo J. 17（1998）：3807-3815. PubMed：9669998. Vanwye，J. D.和K. Haldar. “在疟原虫中的绿色荧光蛋白的表达”. 摩尔.生物学习.寄生醇. 87（1997）：225-229. PubMed：9247934. Crabb，B. S.等人. “稳定的转基因表达在疟原虫疟原虫.” 摩尔.生物学习.寄生醇. 90（1997）：131-144. PubMed：9497038. Mamoun，C. B.等人. “一组独立的可选标记，用于转染人疟疾寄生虫疟原虫疟原虫.” Proc. natl.阿卡. SCI.美国 96（1999）：8716-8720. PUBMED：10411941.
厂牌介绍
BEI Resources 由美国国家过敏和传染病研究所 ( NIAID )成立，旨在为研究 A、B 和 C 类优先病原体、新兴传染病病原体、非病原微生物和其他相关微生物材料提供试剂、工具和信息到研究界。BEI Resources 获取、验证和生产科学家进行基础研究和开发改进的诊断测试、疫苗和疗法所需的试剂。通过将这些功能集中在 BEI Resources 中，可以监控科学界对这些材料的访问和使用，并确保试剂的质量控制。
除了为传染病界提供材料外，BEI Resources 还鼓励和支持研究人员和机构的材料存放。使用 BEI Resources存放材料对研究人员和研究社区有许多优势，包括安全存储、社区访问和分发；同时保护存款人的知识产权。只要有需要，BEI 资源库将作为研究人员的资源进行维护。您在 BEI Resources 的存款是一项有助于未来研究的长期投资。
BEI Resources 自 2003 年起由美国典型培养物保藏中心 (ATCC) 根据合同管理。2016 年 5 月， ATCC获得了一份为期七年的继续管理 BEI Resources的合同。合同范围已扩大到更全面的研究目录材料，包括由其他政府支持的研究项目存放的材料，将提供给生物防御和新兴传染病科学界。真菌、寄生虫、载体和其他相关材料已添加到现有的细菌、病毒和毒素试剂中，涵盖 NIAID A、B 和 C 类优先病原体和 NIAID 指定的新发传染病病原体和生物。
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