NR-50413单克隆抗寨卡病毒包膜(E)蛋白,克隆ZV-16(免疫球蛋白G,小鼠)(单克隆抗体）
产品图片
货号/SKU
NR-50413
货号/规格
EA
库存与交货期
3-8周
人民币价格
14000
试剂海关审批
A/B级风险物质只能直接使用人购买并持有实验室有效资质，其它询客服确认
国外采购
支持/部分限制一年内购买数量
厂牌
BEI Resources(ATCC)
产品基础信息
生物安全等级建议分类：美国、1
产品描述信息
NR-50413?? Monoclonal Anti-Zika Virus Envelope (E) Protein, Clone ZV-16 (immunoglobulin G, Mouse)(Monoclonal Antibodies)|Zika virus|Monoclonal Anti-Zika Virus Envelope (E) Protein, Clone ZV-16 (immunoglobulin G, Mouse)|-20°C or colder|MS DiamondAcknowledgment for publications should read "The following reagent was obtained through BEI Resources, NIAID, NIH: Monoclonal Anti-Zika Virus Envelope (E) Protein, Clone ZV-16 (immunoglobulin G, Mouse), NR-50413."|Quantity limit per order for this item is 1. This item can be ordered twice a year. Orders over this limit will be sent to NIAID for approval before shipment.
This item is currently in our production queue. Please allow ample time for distribution lots to be made available.
Antibody Class: IgG2cκ
Mouse monoclonal antibody prepared against the envelope (E) glycoprotein of Zika virus (ZIKV) was purified from ascites fluid by protein G affinity chromatography. Ascites formation was induced by injecting approximately 2.5 × 106 cultured hybridoma clone ZV-16 cells into the peritoneal cavities of pristane-primed mice. The B cell hybridoma was generated by the fusion of P3X63Ag8.653 mouse myeloma cells with immunized mouse splenocytes. The ZV-16 antibody specifically recognizes the E glycoprotein, is non-neutralizing, and does not cross-react with dengue, Japanese encephalitis, or West Nile viruses.
Each vial of NR-50413 contains approximately 100 ?L of purified monoclonal antibody in phosphate-buffered saline, pH 7.4.
NR-50413 specifically recognizes ZIKV-infected cells in indirect immunofluorescence assays. The clone ZV-16 antibody is also reported to function in ELISA and in western blot assays performed under non-reducing conditions.
NR-50413 is claimed in U.S. Provisional Patent Application number 62/366,782 and the continuations, continuations-in-part, re-issues, and foreign counterparts thereof. For-profit entities wishing to obtain this material must confirm in writing to BEI Resources that they have executed a licensing agreement with the Contributor’s institution. Please contact the Office of Technology Management of the Washington University in St. Louis directly at mta@dom.wustl.edu.
This item is currently in our production queue. Please allow ample time for distribution lots to be made available.
Antibody Class: IgG2cκ
Mouse monoclonal antibody prepared against the envelope (E) glycoprotein of Zika virus (ZIKV) was purified from ascites fluid by protein G affinity chromatography. Ascites formation was induced by injecting approximately 2.5 × 106 cultured hybridoma clone ZV-16 cells into the peritoneal cavities of pristane-primed mice. The B cell hybridoma was generated by the fusion of P3X63Ag8.653 mouse myeloma cells with immunized mouse splenocytes. The ZV-16 antibody specifically recognizes the E glycoprotein, is non-neutralizing, and does not cross-react with dengue, Japanese encephalitis, or West Nile viruses.
Each vial of NR-50413 contains approximately 100 ?L of purified monoclonal antibody in phosphate-buffered saline, pH 7.4.
NR-50413 specifically recognizes ZIKV-infected cells in indirect immunofluorescence assays. The clone ZV-16 antibody is also reported to function in ELISA and in western blot assays performed under non-reducing conditions.
NR-50413 is claimed in U.S. Provisional Patent Application number 62/366,782 and the continuations, continuations-in-part, re-issues, and foreign counterparts thereof. For-profit entities wishing to obtain this material must confirm in writing to BEI Resources that they have executed a licensing agreement with the Contributor’s institution. Please contact the Office of Technology Management of the Washington University in St. Louis directly at mta@dom.wustl.edu.
主要内容
此项目的每个订单数量限制为1.此商品每年可订购两次.通过此限制的订单将在发货前发送至NIAID进行批准.
此项目目前处于我们的生产队列中.请允许充分时间进行分销批次. 抗体类：IgG2Cκ
通过蛋白G亲和层析从腹水流体纯化对Zika病毒（ZIKV）的封套（e）糖蛋白制备的小鼠单克隆抗体.通过将大约2.5×10 6克隆杂交瘤克隆ZV-16细胞注入预丙烷底粒小鼠的腹膜腔中来诱导腹水形成.通过P3X63AG8.653小鼠骨髓瘤细胞的融合产生B细胞杂交瘤，具有免疫小鼠脾细胞. ZV-16抗体特异性识别出E糖蛋白，是不中和的，并且不会与登革热，日本脑炎或西尼罗病毒交叉反应.
NR-50413的每个小瓶中含有大约100μl的磷酸盐缓冲盐水纯化的单克隆抗体，pH 7.4.
NR-50413具体识别间接免疫荧光测定中的Zikv感染的细胞.克隆Zv-16抗体还报告在ELISA中的功能和在非还原条件下进行的蛋白质印迹测定.
NR-50413在美国临时专利申请号62 / 366,782中索赔，持续，持续的，重新发布及其外来同行.希望获得此材料的营利性实体必须以书面形式确认，以便他们与派遣国的机构执行了许可协议.请直接联系华盛顿大学技术管理办公室，直接在 mta@dom.wustl.edu
厂牌介绍
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BEI Resources 自 2003 年起由美国典型培养物保藏中心 (ATCC) 根据合同管理。2016 年 5 月， ATCC获得了一份为期七年的继续管理 BEI Resources的合同。合同范围已扩大到更全面的研究目录材料，包括由其他政府支持的研究项目存放的材料，将提供给生物防御和新兴传染病科学界。真菌、寄生虫、载体和其他相关材料已添加到现有的细菌、病毒和毒素试剂中，涵盖 NIAID A、B 和 C 类优先病原体和 NIAID 指定的新发传染病病原体和生物。
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