NR-53713用C-末端组氨酸和AVI标签的人冠状病毒

NR-53713?? Spike Glycoprotein (Stabilized) from Human Coronavirus, HKU1 with C-Terminal Histidine and Avi Tags, Recombinant from HEK293F Cells(Proteins)|Human Coronavirus|Spike Glycoprotein (Stabilized) from Human Coronavirus, HKU1 with C-Terminal Histidine and Avi Tags, Recombinant from HEK293F Cells| -20°C or colder|RK StrongAcknowledgment for publications should read “The following reagent was obtained through BEI Resources, NIAID, NIH: Spike Glycoprotein (Stabilized) from Human Coronavirus, HKU1 with C-Terminal Histidine and Avi Tags, Recombinant from HEK293F Cells, NR-53713.”|Quantity limit per order for this item is 1. This item can be ordered twice a year. Orders over this limit will be sent to NIAID for approval before shipment. A recombinant form of the spike (S) glycoprotein from human coronavirus (HCoV), HKU1 (GenPept: ABC70719) was produced in human embryonic kidney HEK293F cells and purified by immobilized metal affinity and size exclusion chromatography. NR-53713 lacks the signal sequence and contains 1264 residues (ectodomain) of the HCoV spike glycoprotein; the recombinant protein was stabilized by substitution at the furin S1/S2 cleavage site (RRKRR to GGSGS; residues 752 to 756) and with a pair of mutations (N1067P and L1068P, wild type numbering), and includes a thrombin cleavage site, T4 foldon trimerization domain and C-terminal hexa-histidine tag fused to an AviTag? BirA biotinylation acceptor sequence. NR-53713 has a theoretical molecular weight of 147,070 daltons. The crystal structure for trimeric S glycoprotein from HCoV, HKU1 has been solved at 4.04 ? resolution (PDB: 5I08). The S glycoprotein mediates viral binding to the host angiotensin converting enzyme 2 (ACE2). This protein forms a trimer, and when bound to a host receptor allows fusion of the viral and cellular membranes. The S protein is a target for neutralizing antibodies. Each vial contains approximately 50 ?L of NR-53713 in phosphate buffered saline (PBS; pH ~ 7). Additional information and tools are available at ViPR (Virus Pathogen Resource).