NR-52967载体pLVX-EF1α-IRES-Puro含有SARS相关冠状病毒2,USA-WA1/2020包膜基因(质粒/载体）
产品图片
货号/SKU
NR-52967
货号/规格
EA
库存与交货期
3-8周
人民币价格
14000
试剂海关审批
A/B级风险物质只能直接使用人购买并持有实验室有效资质，其它询客服确认
国外采购
支持/部分限制一年内购买数量
厂牌
BEI Resources(ATCC)
产品基础信息
生物安全等级建议分类：美国、1
产品描述信息
NR-52967?? Vector pLVX-EF1α-IRES-Puro Containing the SARS-Related Coronavirus 2, USA-WA1/2020 Envelope Gene(Plasmid/Vectors)|SARS-Related Coronavirus 2|Vector pLVX-EF1α-IRES-Puro Containing the SARS-Related Coronavirus 2, USA-WA1/2020 Envelope Gene| -20°C or colder|N KroganAcknowledgment for publications should read “The following reagent was obtained through BEI Resources, NIAID, NIH: Vector pLVX-EF1α-IRES-Puro Containing the SARS-Related Coronavirus 2, USA-WA1/2020 Envelope Gene, NR-52967.”|Quantity limit per order for this item is 1. This item can be ordered twice a year. Orders over this limit will be sent to NIAID for approval before shipment.
Note: The vial label indicates this product contains a TST tag. This nomenclature refers to a 2X Strep tag. This product does not express the Twin-Strep-tag? that is commonly referred to as a TST tag.
The envelope (E) gene from severe acute respiratory syndrome-related coronavirus 2 (SARS-CoV-2), USA-WA1/2020 (GenBank: MN985325) was codon optimized and modified by the addition of a C-terminal 2X Strep tag and cloned into the pLVX-EF1α-IRES-Puro lentiviral expression plasmid. The vector contains an internal ribosomal entry site (IRES) that allows a gene-of-interest and a puromycin resistance gene to be simultaneously co expressed from a single mRNA transcript. Expression of the transcript is driven by the human elongation factor 1 alpha (EF1α) promoter. The beta-lactamase gene, bla, provides transformant selection through ampicillin resistance in Escherichia coli (E. coli) and the puromycin resistance gene, pac, provides transformant selection through puromycin resistance in eukaryotic cells. The resulting size of the plasmid is approximately 9740 base pairs. NR-52967 can be used for transient expression and lentivirus generation. The complete plasmid sequence and map are provided on the BEI Resources webpage. The plasmid was produced in E. coli and extracted.
The E protein is one of the four major structural protein and is involved in viral assembly and virion release. It is required for efficient movement of virions through the host secretory pathway. The E protein has been identified as playing a role in regulation of host cell response.
Each vial contains plasmid DNA in TE buffer (10 mM Tris-HCl, 1 mM EDTA, pH 8.0). The vial should be centrifuged prior to opening. Note: The contents of the vial should be used to replicate the plasmid in E. coli prior to mammalian expression studies.
Note: The vial label indicates this product contains a TST tag. This nomenclature refers to a 2X Strep tag. This product does not express the Twin-Strep-tag? that is commonly referred to as a TST tag.
The envelope (E) gene from severe acute respiratory syndrome-related coronavirus 2 (SARS-CoV-2), USA-WA1/2020 (GenBank: MN985325) was codon optimized and modified by the addition of a C-terminal 2X Strep tag and cloned into the pLVX-EF1α-IRES-Puro lentiviral expression plasmid. The vector contains an internal ribosomal entry site (IRES) that allows a gene-of-interest and a puromycin resistance gene to be simultaneously co expressed from a single mRNA transcript. Expression of the transcript is driven by the human elongation factor 1 alpha (EF1α) promoter. The beta-lactamase gene, bla, provides transformant selection through ampicillin resistance in Escherichia coli (E. coli) and the puromycin resistance gene, pac, provides transformant selection through puromycin resistance in eukaryotic cells. The resulting size of the plasmid is approximately 9740 base pairs. NR-52967 can be used for transient expression and lentivirus generation. The complete plasmid sequence and map are provided on the BEI Resources webpage. The plasmid was produced in E. coli and extracted.
The E protein is one of the four major structural protein and is involved in viral assembly and virion release. It is required for efficient movement of virions through the host secretory pathway. The E protein has been identified as playing a role in regulation of host cell response.
Each vial contains plasmid DNA in TE buffer (10 mM Tris-HCl, 1 mM EDTA, pH 8.0). The vial should be centrifuged prior to opening. Note: The contents of the vial should be used to replicate the plasmid in E. coli prior to mammalian expression studies.
主要内容
此项目的每个订单数量限制为1.此商品每年可订购两次.通过此限制的订单将在发货前发送至NIAID进行批准.
注意：小瓶标签表示本产品包含TST标记. 该命名法是指2x strep标签. 本产品不会表达通常称为TST标记的双击标签?.
来自严重急性呼吸综合征相关的冠状病毒2（SARS-COV-2），USA-WA1 / 2020（Genbank： Mn985325 ）是密码子通过添加C终端2x Strep标签来优化和修改，并克隆到 PLVX-EF1α-IRES-PURO 慢病毒表达质粒.载体含有内部核糖体进入位点（IRE），其允许兴趣基因和嘌呤霉素抗性基因同时与单个mRNA转录物表达.转录物的表达由人伸长因子1α（EF1α）启动子驱动. β-内酰胺酶基因 BLA 通过在大肠杆菌中通过氨苄青霉素抗性提供转化体选择
COLI（大肠杆菌）和嘌呤霉素抗性基因， PAC ，通过真核细胞中的嘌呤霉素抗性提供转化体选择.得到的质粒尺寸约为9740碱基对. NR-52967可用于瞬时表达和慢病毒生成.在北部资源网页上提供完整的质粒序列和地图.在 e中制备质粒. Coli 并提取.
E蛋白是四个主要结构蛋白之一，参与病毒组装和病毒虫释放.需要通过主体分泌途径有效地运动.已被鉴定为在宿主细胞反应调节中发挥作用.
每个小瓶在Te缓冲液中含有质粒DNA（10mM Tris-HCl，1mM EDTA，pH8.0）.在打开之前应该离心小瓶. 注意：Vial的内容应该用于复制 e中的质粒. Coli 在哺乳动物表达研究之前.
厂牌介绍
BEI Resources 由美国国家过敏和传染病研究所 ( NIAID )成立，旨在为研究 A、B 和 C 类优先病原体、新兴传染病病原体、非病原微生物和其他相关微生物材料提供试剂、工具和信息到研究界。BEI Resources 获取、验证和生产科学家进行基础研究和开发改进的诊断测试、疫苗和疗法所需的试剂。通过将这些功能集中在 BEI Resources 中，可以监控科学界对这些材料的访问和使用，并确保试剂的质量控制。
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BEI Resources 自 2003 年起由美国典型培养物保藏中心 (ATCC) 根据合同管理。2016 年 5 月， ATCC获得了一份为期七年的继续管理 BEI Resources的合同。合同范围已扩大到更全面的研究目录材料，包括由其他政府支持的研究项目存放的材料，将提供给生物防御和新兴传染病科学界。真菌、寄生虫、载体和其他相关材料已添加到现有的细菌、病毒和毒素试剂中，涵盖 NIAID A、B 和 C 类优先病原体和 NIAID 指定的新发传染病病原体和生物。
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