NR-51634 白色念珠菌

NR-51634??Candida albicans, CAI4-F2-Neut5L-NAT1-mCherry-GFP(Fungi)|Candida albicans|CAI4-F2-Neut5L-NAT1-mCherry-GFP| -70°C or colder |RP RaoAcknowledgment for publications should read “The following reagent was obtained through BEI Resources, NIAID, NIH: Candida albicans, Strain CAI4-F2-Neut5L-NAT1-mCherry-GFP, NR-51634.”|Quantity limit per order for this item is 1. This item can be ordered twice a year. Orders over this limit will be sent to NIAID for approval before shipment. For information on purchasing a license to use this product for research (for-profit entities) or commercial purposes (any entity) other than those permitted in the limited use label license, please contact a licensing representative at Takara Bio USA, Inc., by phone at 650.919.7320 or by e-mail at licensing@takarabio.com. Candida albicans (C. albicans), strain CAI4-F2-Neut5L-NAT1-mCherry-GFP was deposited to BEI Resources as a transgenic reporter strain that constitutively expresses green fluorescent protein (GFP) and red fluorescent protein via mCherry. Strain CAI4-F2-Neut5L-NAT1-mCherry-GFP was engineered by integrating the GFP and mCherry fluorescent tags, driven by the bidirectional ADH1 promoter, and a nourseothricin resistance (NATR) cassette at the Neut5L locus of C. albicans, strain CAI4-F2. Strain CAI4-F2 is a homozygous URA3 deletion mutant of strain CAI4, which was derived from the parental strain SC5314, a clinical isolate collected in the 1980s from a patient with generalized candidiasis in New York, USA. C. albicans, strain CAI4-F2-Neut5L-NAT1-mCherry-GFP is reported as genotype ura3::imm434/ura3::imm434 iro1/iro1::imm434 and has been used as an in vitro system for fluorescent sorting of C. albicans with macrophages. Each vial contains approximately 0.5 mL of yeast culture in 20% glycerol.