NR-4961_来自肠道病毒71(EV-71)、MP4(核酸)的基因组RNA
产品图片
货号/SKU
NR-4961
货号/规格
EA
库存与交货期
3-8周
人民币价格
14000
试剂海关审批
A/B级风险物质只能直接使用人购买并持有实验室有效资质,其它询客服确认
国外采购
支持/部分限制一年内购买数量
厂牌
BEI Resources(ATCC)
产品基础信息
生物安全等级建议分类:美国、1
产品描述信息
NR-4961?? Genomic RNA from Enterovirus 71 (EV-71), MP4(Nucleic Acids)|Enterovirus 71|Genomic RNA from Enterovirus 71 (EV-71), MP4|-60°C or colder|H-Y LeiAcknowledgment for publications should read "The following reagent was obtained through BEI Resources, NIAID, NIH: Genomic RNA from Enterovirus 71 (EV-71), MP4, NR-4961."|Quantity limit per order for this item is 1. This item can be ordered twice a year. Orders over this limit will be sent to NIAID for approval before shipment.
Genomic RNA was isolated from a preparation of cell lysate and supernatant from African green monkey (Vero) cells infected with enterovirus 71 (EV-71), MP4.
EV-71, MP4 was derived from the existing EV-71, Tainan/4643/1998 after four serial passages in mice. EV-71 is a small, non-enveloped, icosahedral virus with a single-stranded, ~ 7.5 kb RNA genome of positive polarity. The single open reading frame encodes a large polyprotein of ~ 2200 amino acids and is flanked by untranslated regions at the 5’ and 3’ ends. The complete genome of the parental EV-71, Tainan/4643/1998 has been sequenced (GenBank: AF304458).
NR-4961 has been qualified for PCR applications by amplification of an approximately 1200 nucleotide sequence.
Each vial contains 100 ?L of viral genomic RNA in TE buffer (10 mM Tris-HCl, 1 mM EDTA, pH 7.0) and may contain sodium azide. The viral genomic RNA is in a background of cellular nucleic acid and carrier RNA.
Genomic RNA was isolated from a preparation of cell lysate and supernatant from African green monkey (Vero) cells infected with enterovirus 71 (EV-71), MP4.
EV-71, MP4 was derived from the existing EV-71, Tainan/4643/1998 after four serial passages in mice. EV-71 is a small, non-enveloped, icosahedral virus with a single-stranded, ~ 7.5 kb RNA genome of positive polarity. The single open reading frame encodes a large polyprotein of ~ 2200 amino acids and is flanked by untranslated regions at the 5’ and 3’ ends. The complete genome of the parental EV-71, Tainan/4643/1998 has been sequenced (GenBank: AF304458).
NR-4961 has been qualified for PCR applications by amplification of an approximately 1200 nucleotide sequence.
Each vial contains 100 ?L of viral genomic RNA in TE buffer (10 mM Tris-HCl, 1 mM EDTA, pH 7.0) and may contain sodium azide. The viral genomic RNA is in a background of cellular nucleic acid and carrier RNA.
主要内容
此项目的每个订单数量限制为1.此商品每年可订购两次.此限制的订单将在发货前发送到NIAID批准.
从肠道病毒71(EV-71)的非洲绿猴(Vero)细胞的细胞裂解物和上清液中分离出基因组RNA,从非洲绿猴(VERO)细胞,MP4.
EV-71,MP4源自在小鼠四次串行通道后的现有EV-71,Tainan / 4643/1998. EV-71是一种小型,无封闭的ICOSaheShral病毒,具有单链,?7.5kb的正极基因组.单个开放阅读框编码大的聚丙烯蛋白为约2200氨基酸,并且在5'和3'末端的未转换区域侧翼.父母ev-71,tainan / 4643/1998的完整基因组(Genbank: af304458 ).
NR-4961通过扩增大约1200个核苷酸序列,已获得PCR应用.
每个小瓶在Te缓冲液中含有100μl病毒基因组RNA(10mM Tris-HCl,1mM EDTA,pH7.0),并且可以含有叠氮化钠.病毒基因组RNA在细胞核酸和载体RNA的背景中.
厂牌介绍
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