线性化(质粒/载体），NR-1_Vaccinia virus

NR-9343?? Plasmid Containing a Segment of the E9L Gene from Vaccinia Virus, New York City Board of Health, Linearized(Plasmid/Vectors)|Vaccinia virus|Plasmid Containing a Segment of the E9L Gene from Vaccinia Virus, New York City Board of Health, Linearized|-60°C or colder|NIH Biodefense and Emerging Infections Research Resources RepositoryAcknowledgment for publications should read "The following reagent was obtained through BEI Resources, NIAID, NIH: Plasmid Containing a Segment of the E9L Gene from Vaccinia Virus, New York City Board of Health, Linearized, NR-9343."|Quantity limit per order for this item is 1. This item can be ordered twice a year. Orders over this limit will be sent to NIAID for approval before shipment.A segment of the E9L gene from vaccinia virus, New York City Board of Health (BEI Resources NR-54) was amplified from extracted DNA and cloned into a commercial vector. The plasmid was produced in Escherichia coli DH5α?-T1^R cells, extracted using a QIAGEN^? Plasmid Maxi Kit and linearized with HindIII.NR-9343 has been qualified for use as a standard for real-time quantitative PCR. A Pan-Orthopox Virus E9L Gene-Specific Quantitative PCR Assay Detection Kit containing forward and reverse primers, probe and NR-9343 is available as BEI Resources NR-9350.Each vial contains approximately 45 ng of plasmid DNA in TE buffer (10 mM Tris-HCl, 1 mM EDTA, pH 7.0).

NR-4076?? Plasmid Containing Hemagglutinin Gene from Monkeypox Virus, Zaire 79, Linearized(Plasmid/Vectors)|Monkeypox virus|Plasmid Containing Hemagglutinin Gene from Monkeypox Virus, Zaire 79, Linearized|-60°C or colder|BEI ResourcesAcknowledgment for publications should read "The following reagent was obtained through BEI Resources, NIAID, NIH: Plasmid Containing the Hemagglutinin Gene from Monkeypox Virus, Zaire 79, Linearized, NR-4076."|Quantity Limit per Order for this item is 1. This item can be ordered twice a year. Orders over this limit will be sent to NIAID for approval before shipment.The hemagglutinin (HA) gene from monkeypox virus, Zaire 79 (BEI Resources NR-2324) was amplified from extracted DNA and cloned into the commercial vector. The plasmid was produced in Escherichia coli DH5alpha?-T1^R cells, extracted using a QIAGEN Plasmid Maxi Kit and linearized with HindIII. The sequence of the HA gene of NR-4076 differs from the monkeypox virus, Zaire 79 HA sequence (GenBank: DQ011155) by one nucleotide at position 516 (T to C).NR-4076 has been qualified for use as a standard for real-time quantitative PCR. A Monkeypox Virus Hemagglutinin Gene-Specific Quantitative PCR Assay Detection Kit containing forward and reverse primers, probe and NR-4076 is available as BEI Resources NR-9351.Each vial contains approximately 100 ?L of plasmid DNA in TE buffer (10 mM Tris-HCl, 1 mM EDTA, pH 7.0).