伽马辐照(灭活生物体），NR-1_Vaccinia virus

NR-56129?? SARS-Related Coronavirus 2, Isolate hCoV-19/USA/MD-HP05285/2021 (Lineage B.1.617.2; Delta Variant), Gamma-Irradiated(Inactivated Organisms)|SARS-Related Coronavirus 2|SARS-Related Coronavirus 2, Isolate hCoV-19/USA/MD-HP05285/2021 (Lineage B.1.617.2; Delta Variant), Gamma-Irradiated|-60°C or colder|AS PekoszAcknowledgment for publications should read “The following reagent was obtained through BEI Resources, NIAID, NIH: SARS-Related Coronavirus 2, Isolate hCoV-19/USA/MD-HP05285/2021 (Lineage B.1.617.2; Delta Variant), Gamma-Irradiated, NR 56129, contributed by Andrew S. Pekosz."|Quantity limit per order for this item is 1. This item can be ordered twice a year. Orders over this limit will be sent to NIAID for approval before shipment. NR-56129 consists of a crude preparation of cell lysate and supernatant from Homo sapiens lung adenocarcinoma epithelial cells (Calu-3) infected with severe acute respiratory syndrome-related coronavirus 2 (SARS CoV 2), isolate hCoV-19/USA/MD-HP05285/2021 that was gamma irradiated (5 × 10⁶ RADs) on dry ice, followed by sonication. SARS-CoV-2, isolate hCoV-19/USA/MD-HP05285/2021 was isolated from a human in Maryland, USA on April 26, 2021. The complete genome of SARS-CoV-2, hCoV-19/USA/MD-HP05285/2021 has been sequenced (GISAID: EPI_ISL_2103264). Each vial contains approximately 0.5 mL of gamma-irradiated and sonicated cell lysate and supernatant from Calu-3 cells infected with SARS-CoV-2, isolate hCoV-19/USA/MD-HP05285/2021.

NR-53258??Cercopithecus aethiops, Vero E6 Cell Lysate Control, Gamma-Irradiated(Inactivated Organisms)|Cercopithecus aethiops|Vero E6 Cell Lysate Control, Gamma-Irradiated| -60°C or colder|ATCC?Acknowledgment for publications should read "The following reagent was obtained through BEI Resources, NIAID, NIH: Vero E6 Cell Lysate Control, Gamma-Irradiated, NR-53258."|Quantity limit per order for this item is 1. This item can be ordered twice a year. Orders over this limit will be sent to NIAID for approval before shipment. NR-53258 consists of a crude preparation of cell lysate and supernatant from Cercopithecus aethiops kidney epithelial cells (Vero E6; ATCC ^? CRL-1586?) that was gamma-irradiated (5 × 10⁶ RADs) on dry ice followed by sonication to clarify the solution. NR-53258 is intended to be used as a control for gamma-irradiated viruses grown in Vero E6 cells. Each vial contains approximately 0.5 mL of gamma-irradiated cell lysate and supernatant from Vero E6 cells.

NR-52287?? SARS-Related Coronavirus 2, Isolate USA-WA1/2020, Gamma-Irradiated(Inactivated Organisms)|SARS-Related Coronavirus 2|SARS-Related Coronavirus 2, Isolate USA-WA1/2020, Gamma-Irradiated|-60°C or colder|CDCAcknowledgment for publications should read "The following reagent was obtained through BEI Resources, NIAID, NIH: SARS-Related Coronavirus 2, Isolate USA-WA1/2020, Gamma-Irradiated, NR-52287, contributed by the Centers for Disease Control and Prevention."|Quantity limit per order for this item is 1. This item can be ordered twice a year. Orders over this limit will be sent to NIAID for approval before shipment. NR-52287 consists of a crude preparation of cell lysate and supernatant from Cercopithecus aethiops kidney epithelial cells (Vero E6; ATCC ^? CRL-1586?) infected with severe acute respiratory syndrome-related coronavirus 2 (SARS-CoV-2), isolate USA-WA1/2020 that was gamma-irradiated (5 × 10⁶ RADs) on dry ice, followed by sonication. SARS-CoV-2, isolate USA-WA1/2020 was isolated from an oropharyngeal swab from a patient with a respiratory illness who had recently returned from travel to the affected region of China and developed clinical disease (COVID-19) in January 2020 in Washington, USA. The complete genome of SARS-CoV-2, USA-WA1/2020 has been sequenced (the isolate - GenBank: MN985325 and GISAID: EPI_ISL_404895 and after one passage in Vero cells - GenBank: MT020880). The complete genome of SARS-CoV-2, USA-WA1/2020 has been sequenced after four passages in Vero cells in collaboration with Database for Reference Grade Microbial Sequences (FDA-ARGOS; GenBank: MT246667). Intended Use: NR-52287 is intended for use in molecular assays or can be used as a process (work-flow) control (for example: viral RNA extraction through qPCR amplification process). Please refer to the Certificate of Analysis for pre-inactivation infectious titer and post-inactivated genome equivalence (GE) quantification by Droplet Digital PCR for your specific lot. The GE value is contributed by amplification of genome fragments and should be treated as an estimate for end-users to qualify assays on their platform. NR-52287 can also be used to make a dilution series to estimate level of detection. However, this product is not suitable as a whole cell antigen preparation because the protein content is largely contributed by the host cell and the fetal bovine serum used during virus propagation. Each vial contains approximately 0.5 mL of gamma-irradiated and sonicated cell lysate and supernatant from Vero E6 cells infected with SARS-CoV-2, isolate USA-WA1/2020. Additional information and tools are available at ViPR (Virus Pathogen Resource).