NR-15781结核分枝杆菌、CDC1551、转座子突变体敲除池9(突变细菌)
产品图片
货号/SKU
NR-15781
货号/规格
EA
库存与交货期
3-8周
人民币价格
14000
试剂海关审批
A/B级风险物质只能直接使用人购买并持有实验室有效资质,其它询客服确认
国外采购
支持/部分限制一年内购买数量
厂牌
BEI Resources(ATCC)
产品基础信息
生物安全等级建议分类:美国、3
产品描述信息
NR-15781??Mycobacterium tuberculosis, CDC1551, Transposon Mutant Knock-Out Pool 9(Mutant Bacteria)|Mycobacterium tuberculosis|CDC1551, Transposon Mutant Knock-Out Pool 9|-60°C or colder|WR BishaiAcknowledgment for publications should read "The following reagent was obtained through BEI Resources, NIAID, NIH: Mycobacterium tuberculosis, Strain CDC1551, Transposon Mutant Knock-Out Pool 9, NR-15781."|Quantity limit per order for this item is 1. This item can be ordered twice a year. Orders over this limit will be sent to NIAID for approval before shipment.
In 2002, TARGET (Tuberculosis Animal Research and Gene Evaluation Taskforce) was formed to enable the modeling of human tuberculosis in multiple animal species using defined protocols and testing defined mutants of Mycobacterium tuberculosis (M. tuberculosis). In addition to animal modeling activities, a library of intragenic transposon mutants has been created and characterized.
There are 20 transposon mutant knock-out pools available from BEI Resources (NR-15773 to NR-15792) that are companion products to the DeADMAn DNA Microarray (available from BEI Resources as NR-18958). The DeADMAn DNA Microarray is used for identification of genes essential for the survival of a stress condition in an in vivo model system infection.
M. tuberculosis, strain CDC1551 transposon mutant knock-out pool 9 is reported to be a mixture of 20 genetically defined M. tuberculosis transposon mutants. Some of the transposon mutants in knock-out pool 9 are available individually (see Pool 9 components below).
Each vial contains approximately 1 mL of bacterial culture in Middlebrook 7H9 broth with OADC enrichment containing 100 ?g/mL cycloheximide and 20 ?g/mL kanamycin.
Components:
In 2002, TARGET (Tuberculosis Animal Research and Gene Evaluation Taskforce) was formed to enable the modeling of human tuberculosis in multiple animal species using defined protocols and testing defined mutants of Mycobacterium tuberculosis (M. tuberculosis). In addition to animal modeling activities, a library of intragenic transposon mutants has been created and characterized.
There are 20 transposon mutant knock-out pools available from BEI Resources (NR-15773 to NR-15792) that are companion products to the DeADMAn DNA Microarray (available from BEI Resources as NR-18958). The DeADMAn DNA Microarray is used for identification of genes essential for the survival of a stress condition in an in vivo model system infection.
M. tuberculosis, strain CDC1551 transposon mutant knock-out pool 9 is reported to be a mixture of 20 genetically defined M. tuberculosis transposon mutants. Some of the transposon mutants in knock-out pool 9 are available individually (see Pool 9 components below).
Each vial contains approximately 1 mL of bacterial culture in Middlebrook 7H9 broth with OADC enrichment containing 100 ?g/mL cycloheximide and 20 ?g/mL kanamycin.
Components:
Description of Transposon Knock-Out Mutant | Strain CDC1551 Gene | Strain H37Rv Gene1 | BEI Resources Product Number2 |
---|---|---|---|
POSSIBLE L-FUCULOSE PHOSPHATE ALDOLASE | MT0752 | Rv0727 | NA |
PROBABLE INTEGRAL MEMBRANE PROTEIN | MT3585 | Rv3481c | NR-13469 |
PROBABLE TRANSCRIPTIONAL REPRESSOR | MT2858 | Rv2788 | NA |
PROBABLE CONSERVED MEMBRANE PROTEIN | MT1416 | Rv1371 | NR-17587 |
PROBABLE GLYCOSYLTRANSFERASE | MT1577 | Rv1526c | NA |
PROBABLE CYCLASE | MT2509 | Rv2435c | NR-15655 |
PROBABLE DEHYDROGENASE | MT2820 | Rv2750 | NR-15703 |
CONSERVED 13E12 REPEAT FAMILY PROTEIN | MT0404 | Rv0393 | NR-15651 |
POSSIBLE COA-TRANSFERASE | MT3656 | Rv3552 | NR-15654 |
CONSERVED HYPOTHETICAL PROTEIN | MT2081 | Rv* | NA |
GLUTAMATE-CYSTEINE LIGASE | MT3807 | Rv3704c | NA |
PROBABLE CATECHOL-O-METHYLTRANSFERASE | MT1743 | Rv1703c | NR-15725 |
POSSIBLE AMIDASE | MT3264 | Rv3175 | NA |
POSSIBLE TRANSPOSASE | MT3534 | Rv3430c | NR-15726 |
CONSERVED HYPOTHETICAL PROTEIN | MT1568 | Rv1518 | NA |
CONSERVED HYPOTHETICAL PROTEIN | MT2359 | Rv2302 | NR-15735 |
HYPOTHETICAL PROTEIN | MT1188 | Rv1154c | NR-18217 |
PROBABLE CONSERVED TRANSMEMBRANE PROTEIN | MT3888 | Rv3779 | NA |
HYPOTHETICAL PROTEIN | MT1945 | Rv* | NR-15730 |
HYPOTHETICAL PROTEIN | MT0640 | Rv0610c | NR-15733 |
1Rv*- In some cases there is no M. tuberculosis, strain H37Rv homologue to the M. tuberculosis, strain CDC1551 gene | |||
2NA- Individual transposon mutant not available from BEI Resources but may be available from TARGET |
主要内容
此项目的每个订单数量限制为1.此商品每年可订购两次.在此限制上订单将在发货前发送到北欧批准. 目标(结核病形成动物研究和基因评估任务性,以使使用定义的方案和测试分枝杆菌的定义突变体(M.Tuberculosis)的定义突变体来实现多种动物物种中的人结核病建模.除了动物建模活动外,还创建和表征了一种腺体转座子突变体. 有来自BEI资源(NR-15773至NR-15792)的20名转座突变体敲除池产品到Deadman DNA微阵列(可从BEI资源获得NR-18958).死亡DNA微阵列用于鉴定在体内体内系统感染中的胁迫条件的生存基因所必需的基因. m.结核病,菌株CDC1551转座子突变突出池9被据报道为20种遗传定义的 m的混合物.结核病转座突变体.敲除池9中的一些转座子突变体可单独使用(参见下面的池9个组分). 每个小瓶在含有100μg/ ml环己酰胺的OADC富集中含有大约1毫升的细菌培养物和20μg/ ml kanamycin. .customtable {border:0px;单元填充:5px;细胞间距:5px;}.定制TD,TH {边框:0PX;垂直对齐:底部;}#col1 {text-align:left;宽度:70%;}#col2 {text-align:center;宽度:10%;}#col3 {text-align:center;宽度:10%;}#col4 {text-align:center;宽度:10%;} 转座子敲除突变体的描述 菌株 cdc1551 基因 菌株 基因 1 bei资源产品 2 可能的l-岩糖磷酸族醛糖酶 mt0752 RV0727 na 可能的整体膜蛋白 mt3585 rv3481c NR-13469 可能的转录压缩机 MT2858 RV2788 NA 可能保守膜>蛋白质 mt1416 rv1371 可能的糖基转移酶 mt1577 rv1526c na 可能的周期酶 mt2509 rv2435c NR-15655 可能的脱氢酶 mt2820 RV2750 nr-15703 保守13e12重复家庭蛋白 mt0404 rv0393 NR-15651 可能的CoA传送酶 mt3656 RV3552 保守假设蛋白 mt2081 RV * na 谷氨酸 - 半胱氨酸连接酶 mt3807 RV3704C NA 可能的儿茶酚-O-甲基转移酶 mt1743 rv1703c nr-15725 可能酰胺酶 mt3264 RV3175 NA 可能的转座酶 mt3534 rv3430c 保守假设蛋白 mt1568 RV1518 Na 保守假设蛋白 mt2359 RV2302 NR-15735 假设蛋白 mt1188 rv1154c NR-18217 可能保守跨膜蛋白 MT3888 rv3779 na 假设蛋白 mt1945 RV * nr-15730 假设蛋白 mt0640 rv0610c nr -15733 1 rv * - 在某些情况下没有 m.结核病,菌株H37RV同源物至 m.结核病,菌株CDC1551基因 2 na - 从BEI资源不提供的单个转座子突变体,但可以从 target
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