NR-15773结核分枝杆菌、CDC1551、转座子突变体敲除池1(突变细菌)
产品图片
货号/SKU
NR-15773
货号/规格
EA
库存与交货期
3-8周
人民币价格
14000
试剂海关审批
A/B级风险物质只能直接使用人购买并持有实验室有效资质,其它询客服确认
国外采购
支持/部分限制一年内购买数量
厂牌
BEI Resources(ATCC)
产品基础信息
生物安全等级建议分类:美国、3
产品描述信息
NR-15773??Mycobacterium tuberculosis, CDC1551, Transposon Mutant Knock-Out Pool 1(Mutant Bacteria)|Mycobacterium tuberculosis|CDC1551, Transposon Mutant Knock-Out Pool 1||WR BishaiAcknowledgment for publications should read "The following reagent was obtained through BEI Resources, NIAID, NIH: Mycobacterium tuberculosis, Strain CDC1551, Transposon Mutant Knock-Out Pool 1, NR-15773."|Quantity limit per order for this item is 1. This item can be ordered twice a year. Orders over this limit will be sent to NIAID for approval before shipment.
In 2002, TARGET (Tuberculosis Animal Research and Gene Evaluation Taskforce) was formed to enable the modeling of human tuberculosis in multiple animal species using defined protocols and testing defined mutants of M. tuberculosis. In addition to animal modeling activities, a library of intragenic transposon mutants has been created and characterized.
There are 20 transposon mutant knock-out pools available from BEI Resources (NR-15773 to NR-15792) that are companion products to the DeADMAn DNA Microarray (available from BEI Resources as NR-18958). The DeADMAn DNA Microarray is used for identification of genes essential for the survival of a stress condition in an in vivo model system infection.
M. tuberculosis, strain CDC1551 transposon mutant knock-out pool 1 is reported to be a mixture of 20 genetically defined M. tuberculosis transposon mutants. Some of the transposon mutants in knock-out pool 1 are available individually (see Pool 1 components below).
Each vial contains approximately 1 mL of bacterial culture in Middlebrook 7H9 broth with OADC enrichment containing 100 ?g/mL cycloheximide and 20 ?g/mL kanamycin.
Components:
In 2002, TARGET (Tuberculosis Animal Research and Gene Evaluation Taskforce) was formed to enable the modeling of human tuberculosis in multiple animal species using defined protocols and testing defined mutants of M. tuberculosis. In addition to animal modeling activities, a library of intragenic transposon mutants has been created and characterized.
There are 20 transposon mutant knock-out pools available from BEI Resources (NR-15773 to NR-15792) that are companion products to the DeADMAn DNA Microarray (available from BEI Resources as NR-18958). The DeADMAn DNA Microarray is used for identification of genes essential for the survival of a stress condition in an in vivo model system infection.
M. tuberculosis, strain CDC1551 transposon mutant knock-out pool 1 is reported to be a mixture of 20 genetically defined M. tuberculosis transposon mutants. Some of the transposon mutants in knock-out pool 1 are available individually (see Pool 1 components below).
Each vial contains approximately 1 mL of bacterial culture in Middlebrook 7H9 broth with OADC enrichment containing 100 ?g/mL cycloheximide and 20 ?g/mL kanamycin.
Components:
Description of Transposon Knock-Out Mutant | Strain CDC1551 Gene | Strain H37Rv Gene1 | BEI Resources Product Number2 |
---|---|---|---|
PUTATIVE ACETYL HYDROLASE | MT2453 | Rv2385 | NA |
ENHANCED INTRACELLULAR SURVIVAL PROTEIN | MT2489 | Rv2416c | NR-17913 |
HYPOTHETICAL PROTEIN | MT2285.2 | Rv2226 | NR-14708 |
PROBABLE TRANSMEMBRANE SERINE/THREONINE-PROTEIN KINASE | MT2149 | Rv2088 | NR-14741 |
HYPOTHETICAL PROTEIN | MT1854 | Rv1804c | NR-14706 |
POSSIBLE FORMATE HYDROGENLYASE | MT0091 | Rv0084 | NR-17915 |
PUTATIVE AMINO ACID PERMEASE | MT2764 | Rv2690c | NR-15006 |
OXIDOREDUCTASE | MT0156 | Rv0148 | NR-17918 |
PROBABLE PHIRV1 PHAGE PROTEIN | MT3573.3 | Rv1582c | NA |
PROBABLE CONSERVED INTEGRAL MEMBRANE TRANSPORT PROTEIN | MT2395 | Rv2333c | NA |
CONSERVED HYPOTHETICAL PROTEIN | MT0523 | Rv0502 | NA |
PROBABLE FATTY-ACID CoA LIGASE | MT1222 | Rv1185c | NA |
TRANSALDOLASE | MT1495 | Rv1448c | NR-15011 |
HYPOTHETICAL PROTEIN | MT0992.1 | Rv0964c | NR-15014 |
PROBABLE MEMBRANE SUGAR TRANSFERASE | MT0723 | Rv0696 | NA |
HYPOTHETICAL PROTEIN | MT1814.2 | Rv* | NR-15017 |
CONSERVED HYPOTHETICAL PROTEIN | MT2804 | Rv2735c | NR-15019 |
HYPOTHETICAL PROTEIN | MT2316 | Rv* | NR-15020 |
HYPOTHETICAL PROTEIN | MT0472.1 | Rv* | NA |
PROTEIN-EXPORT MEMBRANE PROTEIN | MT2665 | Rv2588c | NR-14707 |
1Rv* - In some cases there is no M. tuberculosis, strain H37Rv homologue to the M. tuberculosis, strain CDC1551 gene | |||
2NA - Individual transposon mutant not available from BEI Resources but may be available from TARGET |
主要内容
此项目的每个订单数量限制为1.此商品每年可订购两次.通过此限制的订单将在发货前发送到NIAID进行批准. target (结核病动物研究和基因评估任务性)形成为使用定义的方案和测试的定义的突变体来实现多种动物物种中的人结核病建模.结核病.除了动物建模活动外,还创建和表征了一种腺体转座子突变体. 有来自BEI资源(NR-15773至NR-15792)的20名转座突变体敲除池产品到Deadman DNA微阵列(可从BEI资源获得NR-18958).死亡DNA微阵列用于鉴定在体内体内系统感染中的胁迫条件的生存基因所必需的基因. m.结核病,菌株CDC1551转座子突变突变池1被据报道为20种遗传定义的 m的混合物.结核病转座突变体.敲除池1中的一些转座突变体可单独使用(参见下面的池1个组分). 每个小瓶中含有大约1毫升含有100μg/ ml环己酰胺的OADC富集的1ml细菌培养物和20μg/ ml卡那霉素. 组件: 描述转座子敲除突变体 菌株 cdc1551基因 菌株H37RV 基因 1 bei资源 产品编号 2 推定乙酰水解酶 mt2453 rv2385 na 增强的细胞内存活蛋白 Mt2489 RV2416C 假设蛋白质 mt2285.2 rv2226 可能的跨膜丝氨酸/苏氨酸 - 蛋白激酶 mt2149 rv2088 nr-14741 假设蛋白 mt1854 rv1804c NR-14706 可能甲状腺型氢术酶 RV0084 nr-17915 推定氨基酸允许释放 mt2764 rv2690c 氧化还原酶 mt0156 rv0148 >nr-17918 可能phirv1 phage蛋白 MT3573.3 RV1582C Na 可能保守的整体膜传输蛋白 MT2395 RV2333C Na 保守假设蛋白 Mt0523 RV0502 Na 可能脂肪酸Coa连接酶 Mt1222 RV1185C transaldolase mt1495 rv1448c 假设蛋白 mt0992.1 rv0964c NR-15014 可能膜糖转移酶 Mt0723 RV0696 Na 假设蛋白 rv*/td> nr-15017 保守假设蛋白 mt2804 rv2735c nr- 15019 假设蛋白 MT2316 RV * 假设蛋白 mt0472.1 RV * Na 蛋白质 - 出口膜蛋白 mt2665 rv2588c NR-14707 1 rv * - 在某些情况下,没有 m.结核病,菌株H37RV同源物至 m.结核病,菌株CDC1551基因 2 na - 从BEI资源不提供的单个转座子突变体,但可以从 target
厂牌介绍
BEI Resources 由美国国家过敏和传染病研究所 ( NIAID )成立,旨在为研究 A、B 和 C 类优先病原体、新兴传染病病原体、非病原微生物和其他相关微生物材料提供试剂、工具和信息到研究界。BEI Resources 获取、验证和生产科学家进行基础研究和开发改进的诊断测试、疫苗和疗法所需的试剂。通过将这些功能集中在 BEI Resources 中,可以监控科学界对这些材料的访问和使用,并确保试剂的质量控制。
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