NR-48855_血吸虫属,从小鼠MicroRNA合成的互补DNA(未暴露于血吸虫物种)(核酸)
产品图片
货号/SKU
NR-48855
货号/规格
EA
库存与交货期
3-8周
人民币价格
14000
试剂海关审批
A/B级风险物质只能直接使用人购买并持有实验室有效资质,其它询客服确认
国外采购
支持/部分限制一年内购买数量
厂牌
BEI Resources(ATCC)
产品基础信息
生物安全等级建议分类:美国、1
产品描述信息
NR-48855??Schistosoma spp., Complementary DNA Synthesized from Mouse MicroRNA (Unexposed to Schistosoma Species)(Nucleic Acids)|Schistosoma spp.|Complementary DNA Synthesized from Mouse MicroRNA (Unexposed to Schistosoma Species)|-20°C or colder|MH HsiehAcknowledgment for publications should read "The following reagent was provided by the NIAID Schistosomiasis Resource Center for distribution through BEI Resources, NIAID, NIH: Complementary DNA Synthesized from Mouse MicroRNA (Unexposed to Schistosoma Species), NR-48855."|Quantity limit per order for this item is 1. This item can be ordered twice a year. Orders over this limit will be sent to NIAID for approval.
Complementary DNA (cDNA) was synthesized from microRNA (miRNA) extracted from pooled plasma of normal mice not exposed to Schistosoma sp.
miRNAs are members of a rapidly growing class of small non-coding RNAs, 19 to 23 nucleotides in length, that are present in a highly stable cell-free form in body fluids of animals. miRNAs play an important role in disease and can serve as biomarkers of Schistosoma infection. miRNA was obtained from uninfected mouse plasma using the NucleoSpin? miRNA Plasma kit (Clontech) which is designed for the isolation of short RNA species from cell free blood plasma or serum. cDNA was synthesized from the miRNA with the Mir-X? miRNA First-Strand Synthesis Kit (Clontech). In the synthesis reaction, miRNAs are poly(A)-tailed using poly(A) polymerase, and then copied using a modified oligo(dT) primer and reverse transcriptase.
Each vial of NR-48855 contains approximately 1 ?g of cDNA in DNase/RNase-free distilled water.
Complementary DNA (cDNA) was synthesized from microRNA (miRNA) extracted from pooled plasma of normal mice not exposed to Schistosoma sp.
miRNAs are members of a rapidly growing class of small non-coding RNAs, 19 to 23 nucleotides in length, that are present in a highly stable cell-free form in body fluids of animals. miRNAs play an important role in disease and can serve as biomarkers of Schistosoma infection. miRNA was obtained from uninfected mouse plasma using the NucleoSpin? miRNA Plasma kit (Clontech) which is designed for the isolation of short RNA species from cell free blood plasma or serum. cDNA was synthesized from the miRNA with the Mir-X? miRNA First-Strand Synthesis Kit (Clontech). In the synthesis reaction, miRNAs are poly(A)-tailed using poly(A) polymerase, and then copied using a modified oligo(dT) primer and reverse transcriptase.
Each vial of NR-48855 contains approximately 1 ?g of cDNA in DNase/RNase-free distilled water.
主要内容
此项目的每个订单数量限制为1.此商品每年可订购两次.该限制的订单将被发送到NIAID进行批准. 从未暴露于 SP. MiRNA是一种快速生长的小型非编码RNA,19至23个核苷酸的长度,其在动物的体液中的高度稳定的无细胞形式中存在. MiRNA在疾病中发挥着重要作用,可以用作血吸虫的生物标志物感染.使用Nucleospin ? miRNA等离子体试剂盒(Clontech)从未感染的小鼠血浆中获得miRNA,其设计用于从细胞无血浆或血清中分离短RNA物种.用miRNA用miR-X?miRNA第一链合成试剂盒(Clontech)合成cDNA.在合成反应中,MIRNA是聚(a) - 使用聚(a)聚合酶,然后使用改性的寡核苷酸(dt)底漆和逆转录酶复制. NR-48855的每个小瓶含有约1 μgcDNA在无DNase / RNase的蒸馏水中.
厂牌介绍
BEI Resources 由美国国家过敏和传染病研究所 ( NIAID )成立,旨在为研究 A、B 和 C 类优先病原体、新兴传染病病原体、非病原微生物和其他相关微生物材料提供试剂、工具和信息到研究界。BEI Resources 获取、验证和生产科学家进行基础研究和开发改进的诊断测试、疫苗和疗法所需的试剂。通过将这些功能集中在 BEI Resources 中,可以监控科学界对这些材料的访问和使用,并确保试剂的质量控制。
除了为传染病界提供材料外,BEI Resources 还鼓励和支持研究人员和机构的材料存放。使用 BEI Resources存放材料对研究人员和研究社区有许多优势,包括安全存储、社区访问和分发;同时保护存款人的知识产权。只要有需要,BEI 资源库将作为研究人员的资源进行维护。您在 BEI Resources 的存款是一项有助于未来研究的长期投资。
BEI Resources 自 2003 年起由美国典型培养物保藏中心 (ATCC) 根据合同管理。2016 年 5 月, ATCC获得了一份为期七年的继续管理 BEI Resources的合同。合同范围已扩大到更全面的研究目录材料,包括由其他政府支持的研究项目存放的材料,将提供给生物防御和新兴传染病科学界。真菌、寄生虫、载体和其他相关材料已添加到现有的细菌、病毒和毒素试剂中,涵盖 NIAID A、B 和 C 类优先病原体和 NIAID 指定的新发传染病病原体和生物。
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