产品描述信息
NR-10150??Toxoplasma gondii, ME49 (B7 clone)(Parasitic Protozoa)|Toxoplasma gondii|ME49 (B7 clone)|-130°C or colder (Note: Do not under any circumstances store vials at temperatures warmer than -70°C. Storage under these conditions will result in the death of the culture.)|LD SibleyAcknowledgment for publications should read "The following reagent was obtained through BEI Resources, NIAID, NIH: Toxoplasma gondii, ME49 (B7 Clone), NR-10150."|Quantity limit per order for this item is 1. This item can be ordered twice a year. Orders over this limit will be sent to NIAID for approval before shipment.ME49 (B7 clone) is also referred to as P and PLK.Toxoplasma gondii (T. gondii), ME49 (originally isolated from a sheep in California and available as BEI Resources NR-362) was passed singly through a cat to obtain a line that was capable of producing oocysts, and then further cloned by limiting dilution to produce the B7 clone.Extensive testing of ME49, ME49 (B7 clone) and PTG (an independent clone of ME49) indicated that ME49 was effectively already a biological clone.T. gondii, ME49 (B7 clone) is the Type II parental strain that was used in a genetic cross with the genotype Type III parental strain CTG ARA-SNF (also known as CTG ARA-AR/SNFR and CEP ARA-AR/SNFR and available as BEI Resources NR-10151) to test the feasibility of inter-strain crosses. Strain CTG ARA-SNF is the product of a genetic cross between singly resistant parental clones of the CTG (CEP) strain obtained by chemical mutagenesis.T. gondii, ME49 (B7 clone) is commonly associated with AIDS. The whole genome shotgun sequence of ME49 (B7 clone) has been deposited in GenBank (ABPA01000000).Each vial of NR-10150 contains approximately 0.5 mL of culture in cryopreservative.