NR-51513单克隆抗沙粒病毒rGPC,克隆KL-AV-2A1(体外生产)(单克隆抗体)
产品图片

货号/SKU
NR-51513
货号/规格
EA
库存与交货期
3-8周
人民币价格
14000
试剂海关审批
A/B级风险物质只能直接使用人购买并持有实验室有效资质,其它询客服确认
国外采购
支持/部分限制一年内购买数量
厂牌
BEI Resources(ATCC)
产品基础信息
生物安全等级建议分类:美国、1
产品描述信息
NR-51513?? Monoclonal Anti-Arenavirus rGPC, Clone KL-AV-2A1 (produced in vitro)(Monoclonal Antibodies)|Arenavirus|Monoclonal Anti-Arenavirus rGPC, Clone KL-AV-2A1 (produced in vitro)| -20°C or colder |F KrammerAcknowledgment for publications should read “The following reagent was obtained through BEI Resources, NIAID, NIH: Monoclonal Anti-Arenavirus rGPC, Clone KL-AV-2A1 (produced in vitro), NR-51513.”|Quantity limit per order for this item is 1. This item can be ordered twice a year. Orders over this limit will be sent to NIAID for approval before shipment.
Antibody Class: IgG2aκ
Mouse monoclonal antibody prepared against both Old World and New World arenavirus recombinant glycoprotein complex (rGPC) was purified from clone KL-AV-2A1 hybridoma supernatant using protein G affinity chromatography. The B cell hybridoma was generated by the fusion of Sp2/0-Ag14 mouse myeloma cells with splenocytes from BALB/c mice sequentially immunized with DNA vaccines encoding ectodomain of glycoprotein from Lassa virus (LASV GPC), followed by Machupo virus (MACV GPC) and Mopeia virus (MOPV GPC) with a final LASV GPC recombinant protein boost.
NR-51513 is reactive in indirect immunofluorescence assays using BSC40 cells infected with recombinant vaccinia viruses expressing glycoproteins from various arenaviruses. The antibody is not neutralizing in vitro and shows no protection from virus challenge in in vivo mouse models. Clone KL-AV-2A1 antibody is also reported to be a broadly cross-reactive anti-arenavirus antibody in ELISA and shows binding to glycoproteins derived from MACV, LASV, White-water Arroyo virus, Tamiami virus, Tacaribe virus and Guanarito virus. It recognizes an epitope from subunit 2 of the glycoprotein complex that is relatively conserved among arenaviruses.
Each vial of NR-51513 contains approximately 100 ?L of purified monoclonal antibody in PBS.
Antibody Class: IgG2aκ
Mouse monoclonal antibody prepared against both Old World and New World arenavirus recombinant glycoprotein complex (rGPC) was purified from clone KL-AV-2A1 hybridoma supernatant using protein G affinity chromatography. The B cell hybridoma was generated by the fusion of Sp2/0-Ag14 mouse myeloma cells with splenocytes from BALB/c mice sequentially immunized with DNA vaccines encoding ectodomain of glycoprotein from Lassa virus (LASV GPC), followed by Machupo virus (MACV GPC) and Mopeia virus (MOPV GPC) with a final LASV GPC recombinant protein boost.
NR-51513 is reactive in indirect immunofluorescence assays using BSC40 cells infected with recombinant vaccinia viruses expressing glycoproteins from various arenaviruses. The antibody is not neutralizing in vitro and shows no protection from virus challenge in in vivo mouse models. Clone KL-AV-2A1 antibody is also reported to be a broadly cross-reactive anti-arenavirus antibody in ELISA and shows binding to glycoproteins derived from MACV, LASV, White-water Arroyo virus, Tamiami virus, Tacaribe virus and Guanarito virus. It recognizes an epitope from subunit 2 of the glycoprotein complex that is relatively conserved among arenaviruses.
Each vial of NR-51513 contains approximately 100 ?L of purified monoclonal antibody in PBS.
主要内容
此项目的每个订单数量限制为1.此商品每年可订购两次.此限制的订单将在发货前发送到NIAID批准.
抗体类:IgG2aκ
使用蛋白G亲和力纯化针对旧世界和新世界areNavirus重组糖蛋白复合物(RGPC)的小鼠单克隆抗体纯化含蛋白G亲和力的杂交瘤上清液
色谱法.通过SP2 / 0-AG14小鼠骨髓瘤细胞的融合产生B细胞杂交瘤
用脾细胞从BALB / C小鼠中依次免疫,与从兰萨病毒(LASV GPC)编码糖蛋白的外胚蛋白的DNA疫苗,然后用MACHUPO病毒(MACV GPC)和MOPV GPC重组蛋白升压.
NR-51513在间接免疫荧光测定中使用感染的BSC40细胞进行间接免疫荧光测定是反应性的,所述BSC40细胞来自表达各种蛛网病毒的糖蛋白的重组疫苗病毒.抗体在体外中没有中和,并且在体内小鼠模型中没有在中的病毒攻击免受保护.据报道,克隆KL-AV-2A1抗体也是ELISA中的广泛反应性抗arenavirus抗体,并显示与衍生自MacV,LASV,白水Arroyo病毒,祖美病毒,塔卡韦斯病毒和胍基因病毒的糖蛋白的结合.它识别来自糖蛋白复合物的亚基2的表位,所述糖蛋白复合物在胰岛素中相对保守.
每个小瓶NR-51513在PBS中含有大约100μl纯化的单克隆抗体.
厂牌介绍
BEI Resources 由美国国家过敏和传染病研究所 ( NIAID )成立,旨在为研究 A、B 和 C 类优先病原体、新兴传染病病原体、非病原微生物和其他相关微生物材料提供试剂、工具和信息到研究界。BEI Resources 获取、验证和生产科学家进行基础研究和开发改进的诊断测试、疫苗和疗法所需的试剂。通过将这些功能集中在 BEI Resources 中,可以监控科学界对这些材料的访问和使用,并确保试剂的质量控制。
除了为传染病界提供材料外,BEI Resources 还鼓励和支持研究人员和机构的材料存放。使用 BEI Resources存放材料对研究人员和研究社区有许多优势,包括安全存储、社区访问和分发;同时保护存款人的知识产权。只要有需要,BEI 资源库将作为研究人员的资源进行维护。您在 BEI Resources 的存款是一项有助于未来研究的长期投资。
BEI Resources 自 2003 年起由美国典型培养物保藏中心 (ATCC) 根据合同管理。2016 年 5 月, ATCC获得了一份为期七年的继续管理 BEI Resources的合同。合同范围已扩大到更全面的研究目录材料,包括由其他政府支持的研究项目存放的材料,将提供给生物防御和新兴传染病科学界。真菌、寄生虫、载体和其他相关材料已添加到现有的细菌、病毒和毒素试剂中,涵盖 NIAID A、B 和 C 类优先病原体和 NIAID 指定的新发传染病病原体和生物。
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