产品描述信息
NR-8034??Francisella tularensis subsp. novicida, "Two-Allele" Transposon Mutant Library, Plates 1-33(Mutant Bacteria)|Francisella tularensis subsp. novicida|"Two-Allele" Transposon Mutant Library, Plates 1-33||C ManoilAcknowledgment for publications should read "The following reagent was obtained through BEI Resources, NIAID, NIH: Francisella tularensis subsp. novicida, "Two-Allele" Transposon Mutant Library, Plates 1-33, NR-8034."|
Quantity limit per order for this item is 1. This item can be ordered twice a year. Orders over this limit will be sent to NIAID for approval before shipment.
Francisella tularensis subsp. novicida, strain U112 is excluded from Select Agent status. Please see CDC Select Agent Program, Notification of Exclusion.
NR-8034 consists of Plates 1-33 (BEI Resources NR-8035 through NR-8066 and NR-10484) of the "two-allele" 3050-member sublibrary. Plate 33 contains 18 strains which were not previously available on Plates 1-32. Eleven strains remain unavailable.
Each well of each 96-well plate contains approximately 0.25 mL of bacterial culture in 0.7X Tryptic Soy Broth containing 0.1% L-cysteine and supplemented with 5% glycerol. The broth also contains kanamycin (10 ?g/mL) except for the strains in wells A04, A05 and A06 of Plate 33 (BEI Resources NR-10484). These 3 strains are erythromycin-resistant and were grown without antibiotic.
Note: Production in the 96-well format has a potential for cross-contamination. Individual mutants should be checked by the recipient prior to use.
Information about specific clones can be accessed through the Francisella Tularensis Genome Research homepage.