- 27 次围观
产品图片
产品别名
货号/SKU
EUI006
货号/规格
120ug
库存与交货期
1-2周
人民币价格
8785
试剂海关审批
使用人负责A/B风险申请资质
国外采购
支持/部分需签MTA
厂牌
Kerafast, Inc.
品牌
产品基础信息
From the laboratory of James H. Morrissey, PhD, University of Michigan.
产品描述信息
Product Type: | Small Molecule |
Name: | Polyphosphate, Long Chain (Biotinylated) |
Chemical Formula: | (NaPO3)n[actual counterions may be Na &/or Li; nis approximately 900 to 1100] |
Molecular Weight: | Heterogeneous; Number average ~100 kDa; Range ~8 kDa to 192 kDa |
Format: | Lyophilized |
Purity: | |
Buffer: | 20 mM Hepes-NaOH pH 8.0, 5 mM EDTA |
Solubility: | At least 100 mM in water; |
Concentration: | When reconstituted:10 mM (phosphate monomer) NOTE: To estimate the polymer concentration, divide the phosphate monomer concentration by the modal polymer length |
Size Distribution: | Mode of n: 900-1100 (as measured by migration on PAGE) |
Comments: | It is recommended to use ~10.2 ug of the biotin-polyphosphate to coat all wells of a streptavidin-coated 96-well plate. |
Storage: |
Dry material should be stored at room temperature (or lower) with dessication. Once reconstituted: - Stable at least 12 hours at room temperature - Stable at least 72 hours at 4C - Store long-term at -80C - Aliquot for repeated use, avoiding repeated freeze-thaw cycles - Do not store in the presence of divalent metal ions |
Shipped: | Ambient temperature |
产品安全信息
Choi SC et al. Phosphoramidate end labeling of inorganic polyphosphates: facile manipulation of polyphosphate for investigating and modulating its biological activities. Biochemistry 49(45):9935-41, 2010.Smith SA et al. Inhibition of polyphosphate as a novel strategy for preventing thrombosis and inflammation. Blood 120(26):5103-10, 2012.Morrissey JH, Choi SH, and Smith S.A. Polyphosphate: an ancient molecule that links platelets, coagulation and inflammation. Blood 119:5972-5979, 2012.Rao NN, Gómez-García MR, and Kornberg A. Inorganic polyphosphate: essential for growth and survival. Annu Rev Biochem. 78:605-647, 2009.Dinarvand P, Hassanian SM, Qureshi SH, Manithody C, Eissenberg JC, Yang L, Rezaie AR. Polyphosphate amplifies proinflammatory responses of nuclear proteins through interaction with receptor for advanced glycation end products and P2Y1 purinergic receptor. Blood. 2014 Feb 6;123(6):935-45.Suess PM, Gomer RH. Extracellular polyphosphate inhibits proliferation in anautocrine negative feedback loop in Dictyostelium discoideum. J Biol Chem. 2016 Aug 12. pii: jbc.M116.737825. View ArticleMorrissey JH. Poly-P as Modulator of Hemostasis, Thrombosis, and Inflammation. Blood 2017 130:SCI-1.An J, Cho J. Potential immune-modulatory effects of wheat phytase on the performance of a mouse macrophage cell line, Raw 264.7, exposed to long-chain inorganic polyphosphate. Asian-Australas J Anim Sci. 2020 May 12. View articleIf you publish research with this product, please let us know so we can cite your paper.
主要内容
该多磷酸盐(长链,生物素化)的尺寸是非均相的,具有〜200-1300〜300次磷酸盐单位的近似聚合物长度。高灯含有共价连接到的生物素,可以固定在三相磷酸根的末端磷酸盐中可以固定在链霉素蛋白涂层上多孔板或用于结合蛋白质与多磷酸二磷酸磷酸盐的珠子,无机正磷酸盐的线性聚合物,其在生物体中普及并在生物学中起着不同的作用,最近被证明是人血液凝固系统的有效调制器。其促凝血活性依赖于聚合物长度;这种高MW多磷酸盐制剂在血浆凝血测定中具有非常高的比活性。除了血浆凝血测定的有用工具之外,多磷酸盐可用于研究细菌生长,毒力和细菌膜功能。可用于研究细菌生长,毒力和细菌膜功能。多磷酸盐,中链(P100)多磷酸,长链(P700)生物素化多磷酸盐,培养基密歇根大学詹姆斯H. Morrissey实验室的链(P100)。
厂牌介绍
关于Kerafast Inc.
Kerafast 是一家位于波士顿的试剂公司,其主要使命是为QuanQiu科学界提供易于使用的独特实验室研究工具。我们的产品组合包括细胞系、抗体、小分子、染料等,其中许多在其他地方无法获得。自 2011 年成立以来,来自全球 190 多个机构的研究人员通过我们的在线平台提供了他们的创新试剂,无需通过传统的材料转让协议流程即可快速获取材料。
我们处理提供实验室的所有销售和运输物流,并从每次销售中返还丰厚的特许权使用费。因此,我们帮助提供实验室节省时间和资源,同时为进一步研究提供额外资金。采购科学家可以更轻松地发现和获取其他地方通常无法获得的独特试剂,同时还可以资助其他研究人员的工作。这创建了一个QuanQiu科学家社区,他们贡献和获取Reagent for the Greater Good,以加速他们自己的研究以及整体科学进步。
2018 年,Kerafast 与Absolute Antibody合并,后者是一家总部位于英国的公司,其愿景是为所有研究人员提供重组抗体技术。此次合并将两家公司聚集在一起,共同致力于改善科学界可用的研究工具的选择。
品牌标识
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