L6-Glut4MYC大鼠肌细胞系

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货号/SKU
ESK202-FP
货号/规格
1 vial
库存与交货期
1-2周
人民币价格
15160
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试剂海关审批
使用人负责A/B风险申请资质
国外采购
支持/部分需签MTA
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Kerafast, Inc.
品牌
产品基础信息
From the laboratory of Amira Klip, PhD, Hospital For Sick Children.
产品描述信息

Product Type: Cell Line
Name: L6-GLUT4myc
Cell Type: Skeletal muscle
Accession ID: CVCL_0P25
Organism: Rat
Morphology: Myoblast/Myotube
Source: Quadriceps
Biosafety Level: II
Growth Conditions: MEM-α (with Ribonucleosides and Deoxyribonucleosides) +10% FBS, 1% Antimycotic +5 ug/ml blasticidin
Subculturing: 2-3 days
Cryopreservation: MEM-α +10% FBS+ 10% DMSO
Comments: The myc-epitope in the GLUT4myc has the amino acid sequence of AEEQKLISEEDLLK
Storage: Liquid nitrogen
Shipped: Dry ice
产品安全信息
Cell Line ReferencesWang Q, Khayat Z, Kishi K, Ebina Y, Klip A. (1998) GLUT4 translocation by insulin in intact muscle cells: detection by a fast and quantitative assay. FEBS Letters 427: 193-7;Ueyama A, Yaworsky KL, Wang Q, Ebina Y, Klip A. (1999) GLUT-4myc ectopic expression in L6 myoblasts generates a GLUT-4-specific pool conferring insulin sensitivity. Am. J. Physiol. Endocrinol. Metab. 277: E572-8;Ishikura S, Antonescu CN, Klip A. (2010) Documenting GLUT4 Exocytosis and Endocytosis in Muscle Cell Monolayers. Curr. Protoc. Cell Biol., John Wiley & Sons, Inc., 46: Unit 15.15: 1-9;Rudich A, Konrad D, Török D, Ben-Romano R, Huang C, Niu W, Garg RR, Wijesekara N, Germinario RJ, Bilan PJ, Klip A. (2003)Indinavir uncovers different contributions of GLUT4 and GLUT1 towards glucose uptake in muscle and fat cells and tissues. Diabetologia 46: 649-58.Huang C, Somwar R, Patel N, Niu W, Török D, Klip A. Sustained exposure of L6 myotubes to high glucose and insulin decreases insulin-stimulated GLUT4 translocation but upregulates GLUT4 activity. Diabetes. 2002 Jul;51(7):2090-8.Application ReferencesPillon NJ, Li YE, Fink LN, Brozinick JT, Nikolayev A, Kuo MS, Bilan PJ, KlipA. (2014) Nucleotides released from palmitate-challenged muscle cells through pannexin-3 attract monocytes. Diabetes. 63: 3815-26.Li Q, Zhu X, Ishikura S, Zhang D, Gao J, Sun Y, Contreras-Ferrat A, Foley KP, Lavandero S, Yao Z, Bilan PJ, Klip A, Niu W. (2014) Ca²? signals promote GLUT4 exocytosis and reduce its endocytosis in muscle cells. Am J Physiol Endocrinol Metab. Jul 15;307(2):E209-24.Sun Y, Chiu TT, Foley KP, Bilan PJ, Klip A. (2014) Myosin Va mediates Rab8A-regulated GLUT4 vesicle exocytosis in insulin-stimulated muscle cells. Mol Biol Cell. 25: 1159-70.Foley KP, Klip A. (2014) Dynamic GLUT4 sorting through a syntaxin-6 compartment in muscle cells is derailed by insulin resistance-causing ceramide. Biol Open. 3: 314-25.Chiu TT, Sun Y, Koshkina A, Klip A. (2013) Rac-1 superactivation triggers insulin-independent glucose transporter 4 (GLUT4) translocation that bypasses signaling defects exerted by c-Jun N-terminal kinase (JNK)- and ceramide-induced insulin resistance. J Biol Chem. 288:17520-31.Pillon NJ, Arane K, Bilan PJ, Chiu TT, Klip A. (2012) Muscle cells challenged with saturated fatty acids mount an autonomous inflammatory response that activates macrophages. Cell Commun Signal. 10: 30.Boguslavsky S, Chiu T, Foley KP, Osorio-Fuentealba C, Antonescu CN, Bayer KU, Bilan PJ, Klip A. (2012) Myo1c binding to submembrane actin mediates insulin-induced tethering of GLUT4 vesicles. Mol Biol Cell. 23: 4065-78.Kewalramani G, Fink LN, Asadi F, Klip A. (2011) Palmitate-activated macrophages confer insulin resistance to muscle cells by a mechanism involving protein kinase C ? and ?. PLoS One. 6: e26947.Sun Y, Bilan PJ, Liu Z, Klip A. (2010) Rab8A and Rab13 are activated by insulin and regulate GLUT4 translocation in muscle cells. Proc Natl Acad Sci U S A. 107: 19909-14.Ishikura S, Antonescu CN, Klip A. (2010) Documenting GLUT4 Exocytosis and Endocytosis in Muscle Cell Monolayers. Curr. Protoc. Cell Biol., John Wiley & Sons, Inc., 46: Unit 15.15: 1-9.Samokhvalov V, Bilan PJ, Schertzer JD, Antonescu CN, Klip A. (2009) Palmitate- and lipopolysaccharide-activated macrophages evoke contrasting insulin responses in muscle cells. Am J Physiol Endocrinol Metab. 296: E37-46.Antonescu CN, Díaz M, Femia G, Planas JV, Klip A. (2008) Clathrin-dependent and independent endocytosis of glucose transporter 4 (GLUT4) in myoblasts: regulation by mitochondrial uncoupling. Traffic. 9: 1173-90.Thong FS, Bilan PJ, Klip A. (2007) The Rab GTPase-activating protein AS160 integrates Akt, protein kinase C, and AMP-activated protein kinase signals regulating GLUT4 traffic. Diabetes. 56: 414-23.Wijesekara N, Tung A, Thong F, Klip A. (2006) Muscle cell depolarization induces again in surface GLUT4 via reduced endocytosis independently of AMPK. Am J Physiol Endocrinol Metab. 290: E1276-86.Ishiki M, Randhawa VK, Poon V, Jebailey L, Klip A. (2005) Insulin regulates the membrane arrival, fusion, and C-terminal unmasking of glucose transporter-4 via distinct phosphoinositides. J Biol Chem. 280: 28792-802.Rudich A, Konrad D, Török D, Ben-Romano R, Huang C, Niu W, Garg RR, Wijesekara N, Germinario RJ, Bilan PJ, Klip A. (2003) Indinavir uncovers different contributions of GLUT4 and GLUT1 towards glucose uptake in muscle and fat cells and tissues. Diabetologia 46: 649-58.Wang Q, Somwar R, Bilan PJ, Liu Z, Jin J, Woodgett JR, Klip A. (1999) Protein kinase B/Akt participates in GLUT4 translocation by insulin in L6 myoblasts. Mol Cell Biol. 19: 4008-18.Wang Q, Khayat Z, Kishi K, Ebina Y, Klip A. (1998) GLUT4 translocation by insulin in intact muscle cells: detection by a fast and quantitative assay. FEBS Letters 427: 193-7.Gao MH, Giamouridis D, Lai NC, Walenta E, Paschoal VA, Kim YC, Miyanohara A, Guo T, Liao M, Liu L, Tan Z, Ciaraldi TP, Schenk S, Bhargava A, Oh DY, Hammond HK. One-time injection of AAV8 encoding urocortin 2 provides long-term resolution of insulin resistance. JCI Insight. 2016 Sep 22;1(15):e88322. View ArticleTunduguru R, Zhang J, Aslamy A, Salunkhe VA, Brozinick JT, Elmendorf JS, Thurmond DC. The actin-related p41ARC subunit contributes to p21-activated kinase-1 (PAK1)-mediated glucose uptake into skeletal muscle cells. J Biol Chem. 2017 Sep 25. pii: jbc.M117.801340. View ArticleYang WM, Min KH, Son YS, Park SW, Lee W. Data on the expression and insulin-stimulated phosphorylation of IRS-1 by miR-96 in L6-GLUT4myc myocytes. Data Brief. 2017 Oct 28;15:728-732. doi: 10.1016/j.dib.2017.10.054. eCollection 2017 Dec. View ArticleKellogg DL 3rd, McCammon KM, Hinchee-Rodriguez KS, Adamo ML, Roman LJ. Neuronal nitric oxide synthase mediates insulin- and oxidative stress-induced glucose uptake in skeletal muscle myotubes. Free Radic Biol Med. 2017 Sep;110:261-269. View ArticleStierwalt HD, Ehrlicher SE, Bergman BC, Robinson MM, Newsom SA. Insulin-stimulated Rac1-GTP binding is not impaired by palmitate treatment in L6 myotubes. Physiol Rep. 2018 Dec;6(24):e13956. View ArticleAzhar S, Bittner S, Hu J, Shen WJ, Cortez Y, Hao X, Han L, Lagerstedt JO, Kraemer FB, Johansson JO. Novel ABCA1 peptide agonists with antidiabetic action. Mol Cell Endocrinol. 2019 Jan 15;480:1-11. View ArticleMin KH, Yang WM, Lee W. Saturated fatty acids-induced miR-424-5p aggravates insulin resistance via targeting insulin receptor in hepatocytes. Biochem Biophys Res Commun. 2018 Sep 10;503(3):1587-1593. View ArticleJaldin-Fincati JR, Bilan PJ, Klip A. GLUT4 Translocation in Single Muscle Cells in Culture: Epitope Detection by Immunofluorescence. Methods Mol Biol. 2018;1713:175-192. View ArticleZhang J, Oh E, Merz KE, Aslamy A, Veluthakal R, Salunkhe VA, Ahn M, Tunduguru R, Thurmond DC. DOC2B promotes insulin sensitivity in mice via a novel KLC1-dependent mechanism in skeletal muscle. Diabetologia. 2019 May;62(5):845-859. doi: 10.1007/s00125-019-4824-2. Epub 2019 Feb 1. View ArticleBone DBJ, Meister J, Knudsen JR, Dattaroy D, Cohen A, Lee R, Lu H, Metzger D, Jensen TE, Wess J. Skeletal Muscle-Specific Activation of G(q) Signaling Maintains Glucose Homeostasis. Diabetes. 2019 Jun;68(6):1341-1352. View ArticleKalinovich A, Dehvari N, Åslund A, et al. Treatment with a β-2-adrenoceptor agonist stimulates glucose uptake in skeletal muscle and improves glucose homeostasis, insulin resistance and hepatic steatosis in mice with diet-induced obesity. Diabetologia. 2020;63(8):1603-1615.View ArticleMin KH, Lee W. Alteration of mitochondrial DNA content modulates antioxidant enzyme expressions and oxidative stress in myoblasts. Korean J Physiol Pharmacol. 2019 Nov;23(6):519-528.View ArticleFujimoto BA, Young M, Carter L, Pang APS, Corley MJ, Fogelgren B, Polgar N. The exocyst complex regulates insulin-stimulated glucose uptake of skeletal muscle cells. Am J Physiol Endocrinol Metab. 2019 Dec 1;317(6):E957-E972.View ArticleMasson SWC, Sorrenson B, Shepherd PR, Merry TL. β-catenin regulates muscle glucose transport via actin remodelling and M-cadherin binding. Mol Metab. 2020 Dec;42:101091. View ArticleIf you publish research with this product, please let us know so we can cite your paper.
主要内容
永生化的大鼠骨骼肌细胞(L6)用Myc-epitope过度表达Glut4,这对于测量Glut4易位是有用的。亮点:L6肌细胞线稳定地表达含有14个氨基酸表位的人C-Myc的Glut4蛋白在其第一个外部外圈仅可用于研究葡萄糖摄取和Glut4易位的细胞模型,而不会透化或分馏可用于新型抗糖尿病的细胞复合筛选可用于通过基于质粒的基因转移和病毒感染方案(逆转录病毒和腺病毒)胰岛素刺激骨骼肌中的胰岛素刺激的胰岛素肌肉的结果主要是由于葡萄糖转运蛋白的转移到细胞表面。 L6细胞最初来自大鼠骨骼肌,作为单核肌细胞繁殖,但可以分化为多核原发性肌管。 Myotubes表达了几种典型的骨骼肌,包括Glut4葡萄​​糖转运蛋白。胰岛素刺激具有高灵敏度和最大反应性的葡萄糖摄取,并且在分化的L6 myotubes和Glut4表达方中,随着L6细胞分化,可以获得这些特性。 L6 myotubes的这些特征是重要的,因为Glut4负责成熟的骨骼肌中的胰岛素依赖性葡萄糖摄取。也可用:L6大鼠肌细胞细胞系列从amira Klip,Phd,Sick Childs医院的实验室。
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