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产品图片
产品别名
货号/SKU
EKC003
货号/规格
1 vial
库存与交货期
咨询客服
人民币价格
16285
试剂海关审批
使用人负责A/B风险申请资质
国外采购
支持/部分需签MTA
厂牌
Kerafast, Inc.
品牌
产品基础信息
From the laboratory of Lynda Bonewald, PhD, University of Missouri - Kansas City.
产品描述信息
Product Type: | Cell Line |
Name: | MLO-A5 |
Cell Type: | Postosteoblast/preosteocyte |
Accession ID: | CVCL_0P24 |
Organism: | Mouse |
Morphology: | Adherent osteoblast-like cell; honeycomb-like mineralized matrix within 7-9 days of culture |
Biosafety Level: | I |
Growth Conditions: |
Proliferation medium: AlphaMEM (containing L-glutamine and deoxyribonucleosides); supplemented with 5% FBS and 5% CS, both heat-inactivated; penicillin-streptomycin at 100U/ml-100ug/ml Differentiation medium: AlphaMEM (L-glutamine and deoxyribonucleosides); supplemented with 10% FBS; penicillin-streptomycin at 100U/ml-100ug/ml; approximately 100µg/ml Ascorbic Acid and 4mM β-glycerophosphate (see Comments). Grown on dishes coated with [0.15 mg/ml] rat tail type I collagen. |
Subculturing: | Maintain stock cultures in proliferation medium under subconfluent conditions on collagen coated plates at 37°C and at 5% CO 2. Passage at ~ 1:10 to 1:20 dilution using 0.05% Trypsin/0.53 mM EDTA every 3-4 days (see Rosser J. et al. Methods Mol Biol. 2012;816:67-81) |
Cryopreservation: | 60% alpha-MEM, 30% FBS, 10% DMSO, at 1 × 10 6 cells/ml/cryovial (see Rosser J. et al. Methods Mol Biol. 2012;816:67-81) |
Storage: | Liquid nitrogen |
Shipped: | Dry ice |
产品安全信息
Kato Y, Windle J,Koop B, Qiao M, Bonewald L. Establishment of an osteocyte-like cell line, MLO-Y4 J Bone Min. Res. 12:2014-2023, 1997.Kato, Y, Boskey, A, Spevak, L, Dallas, M, Hori, M, Bonewald, LF, Establishment of an Osteoid Pre-Osteocyte like cell, MLO-A5, that spontaneously mineralizes in culture without the addition of beta-glycerol phosphate and ascorbic acid. J. Bone Min. Res. 16:1622-1633, 2001.A. Sittichockechaiwut, A.M. Scutt, A.J. Ryan, L.M. Bonewald, G.C. Reilly: Use of rapidly mineralising osteoblasts and short periods of mechanical loading to accelerate matrix maturation in 3D scaffolds. Bone 44: 822-829. 2009.H. Morris, C. Reed, J. Haycock, G.C. Reilly. Osteoblast signalling and matrix responses to dynamic flow. Proceedings of the Institution of Mechanical Engineers: Part H Journal of Engineering in Medicine. 224: 1509-1521. 2010.Rosser J, Bonewald LF. Studying osteocyte function using the cell lines MLO-Y4 and MLO-A5. Methods Mol Biol. 2012;816:67-81.R. M. Delaine-Smith, S. MacNeil G.C. Reilly. Matrix production and collagen structure are enhanced in two types of osteogenic progenitor cells by a simple fluid shear stress stimulus. eCells and Materials. 24: 162-174. 2012.R. M. Delaine-Smith, A. Sittichokechaiwut, G. C. Reilly. Primary cilia respond to fluid shear stress and mediate flow-induced calcium deposition in osteoblasts. FASEB Journal. 28: 430-439. 2014.Khalid S, Yamazaki H, Socorro M, Monier D, Beniash E, Napierala D. Reactive oxygen species (ROS) generation as an underlying mechanism of inorganic phosphate (Pi)-induced mineralization of osteogenic cells. Free Radic Biol Med. 2020 Jun;153:103-111. View articleIf you publish research with this product, please let us know so we can cite your paper.
主要内容
MLO-A5细胞系是对骨细胞分化过程,矿化过程和机械负载对生物矿化的影响的模型.highlights:衍生自转基因小鼠,其中在骨钙的控制下表达永生化T-抗原的转基因小鼠晚骨细胞的启动子表达标记如极高的碱性磷酸酶,骨唾液酸蛋白,第1型受体,以及骨钙素在片材中迅速矿化,不在7-9天内形成“蜂窝”的矿物化基质最多身体中丰富的骨细胞,但也是最具挑战性的研究,因为它们嵌入矿化基质中,使它们难以隔离。该细胞系MLO-A5使得更容易研究骨细胞功能。从密苏里大学Lynda Bonewald的实验室,博士博士 - 堪萨斯城。
厂牌介绍
关于Kerafast Inc.
Kerafast 是一家位于波士顿的试剂公司,其主要使命是为QuanQiu科学界提供易于使用的独特实验室研究工具。我们的产品组合包括细胞系、抗体、小分子、染料等,其中许多在其他地方无法获得。自 2011 年成立以来,来自全球 190 多个机构的研究人员通过我们的在线平台提供了他们的创新试剂,无需通过传统的材料转让协议流程即可快速获取材料。
我们处理提供实验室的所有销售和运输物流,并从每次销售中返还丰厚的特许权使用费。因此,我们帮助提供实验室节省时间和资源,同时为进一步研究提供额外资金。采购科学家可以更轻松地发现和获取其他地方通常无法获得的独特试剂,同时还可以资助其他研究人员的工作。这创建了一个QuanQiu科学家社区,他们贡献和获取Reagent for the Greater Good,以加速他们自己的研究以及整体科学进步。
2018 年,Kerafast 与Absolute Antibody合并,后者是一家总部位于英国的公司,其愿景是为所有研究人员提供重组抗体技术。此次合并将两家公司聚集在一起,共同致力于改善科学界可用的研究工具的选择。
品牌标识
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