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产品图片
货号/SKU
NR-844
货号/规格
EA
库存与交货期
3-8周
人民币价格
14000
试剂海关审批
A/B级风险物质只能直接使用人购买并持有实验室有效资质,其它询客服确认
国外采购
支持/部分限制一年内购买数量
厂牌
BEI Resources(ATCC)
产品基础信息
生物安全等级建议分类:美国、1
产品描述信息
NR-844??Escherichia coli, Monoclonal Anti-Shiga Toxin 1 Subunit B (produced in vitro) (Similar to 13C4) (Monoclonal Antibodies)|Escherichia coli|Monoclonal Anti-Shiga Toxin 1 Subunit B (produced in vitro) (Similar to 13C4) |-20°C or colder|AD O'Brien, JF SinclairAcknowledgment for publications should read "The following reagent was obtained through BEI Resources, NIAID, NIH: Monoclonal Anti-Shiga Toxin 1 Subunit B (produced in vitro) (Similar to 13C4), NR-844."|Quantity limit per order for this item is 1. This item can be ordered twice a year. Orders over this limit will be sent to NIAID for approval before shipment.
Antibody Class: IgG1κ
Mouse monoclonal antibody to the B subunit of Shiga-like toxin 1 from Escherichia coli (E. coli) H30 was purified using protein A (lot 4469046) or protein G (lot 61506759) affinity chromatography from supernatants obtained from a mouse hybridoma clonal cell line (ATCC ? CRL-1794?) that produces monoclonal antibody (MAb) 13C4. The hybridoma cell line was generated by the fusion of SP2/0 myeloma cells with immunized mouse splenocytes.
MAb 13C4 is specific to the B subunit of Shiga-like toxin 1 from E. coli H30 by standard Western blot analysis. MAb 13C4 binds both the denatured and native protein and immunoprecipitates intact labeled toxin. MAb 13C4 neutralizes the cytotoxicity of purified E. coli Shiga-like toxin 1 and Shigella dysenteriae Shiga toxin 1 as well as the Shiga-like toxic activities in crude cell extracts of Shigella flexneri, Vibrio cholerae, Vibrio parahaemolyticus and Salmonella typhimurium. MAb 13C4 has been successfully used in a colony blot assay for the detection of bacterial colonies which produce high levels of Shiga-like toxin 1.
Each vial of lot 4469046 contains approximately 50 ?g of NR-844. Sodium azide (0.05%) was added to the preparation of purified monoclonal antibody as a preservative. Each vial of lot 61506759 contains approximately 10 ?g of NR-844 in PBS and does not contain preservatives.
Antibody Class: IgG1κ
Mouse monoclonal antibody to the B subunit of Shiga-like toxin 1 from Escherichia coli (E. coli) H30 was purified using protein A (lot 4469046) or protein G (lot 61506759) affinity chromatography from supernatants obtained from a mouse hybridoma clonal cell line (ATCC ? CRL-1794?) that produces monoclonal antibody (MAb) 13C4. The hybridoma cell line was generated by the fusion of SP2/0 myeloma cells with immunized mouse splenocytes.
MAb 13C4 is specific to the B subunit of Shiga-like toxin 1 from E. coli H30 by standard Western blot analysis. MAb 13C4 binds both the denatured and native protein and immunoprecipitates intact labeled toxin. MAb 13C4 neutralizes the cytotoxicity of purified E. coli Shiga-like toxin 1 and Shigella dysenteriae Shiga toxin 1 as well as the Shiga-like toxic activities in crude cell extracts of Shigella flexneri, Vibrio cholerae, Vibrio parahaemolyticus and Salmonella typhimurium. MAb 13C4 has been successfully used in a colony blot assay for the detection of bacterial colonies which produce high levels of Shiga-like toxin 1.
Each vial of lot 4469046 contains approximately 50 ?g of NR-844. Sodium azide (0.05%) was added to the preparation of purified monoclonal antibody as a preservative. Each vial of lot 61506759 contains approximately 10 ?g of NR-844 in PBS and does not contain preservatives.
主要内容
此项目的每个订单数量限制为1.此商品每年可订购两次.在此限制上订单将在发货前发送到核心批准. 抗体类:IgG1κ小鼠单克隆抗体从大肠杆菌的Shiga样毒素1的B亚基(大肠杆菌)使用蛋白A(批次4469046)或蛋白G(Lot 61506759)来自由小鼠杂交瘤克隆(ATCC?CRL-1794?)的上清液(ATCC?CRL-1794?)产生的蛋白质G(Lot 61506759)亲和层析纯化.产生单克隆抗体(MAB)13C4.杂交瘤细胞系由具有免疫小鼠脾细胞的SP2 / 0骨髓瘤细胞的融合产生. MAB 13C4特异于S.I> e的Shiga样毒素1的B亚基. COLI H30通过标准Western印迹分析. MAB 13C4结合变性和天然蛋白质和免疫沉淀物完整标记的毒素. MAB 13C4中和纯化的细胞毒性 e. Coli 滋生毒素1和志贺菌痢疾滋生毒素1以及滋生细胞提取物中的滋阴类毒性活性志贺氏菌,霍乱霍乱,血管溶血液,副乙酰氨醇 - vibrio parahaemolyticus 和沙门氏菌伤寒伤寒. MAB 13C4已成功用于菌落印迹测定中,用于检测产生高水平的滋生毒素1. 批次的每个小瓶4469046含有约50μg的NR-844.将叠氮化钠(0.05%)加入到制备纯化的单克隆抗体中作为防腐剂. Lot 61506759的每个小瓶含有大约10μg的PBS NR-844,不含防腐剂.
厂牌介绍
BEI Resources 由美国国家过敏和传染病研究所 ( NIAID )成立,旨在为研究 A、B 和 C 类优先病原体、新兴传染病病原体、非病原微生物和其他相关微生物材料提供试剂、工具和信息到研究界。BEI Resources 获取、验证和生产科学家进行基础研究和开发改进的诊断测试、疫苗和疗法所需的试剂。通过将这些功能集中在 BEI Resources 中,可以监控科学界对这些材料的访问和使用,并确保试剂的质量控制。
除了为传染病界提供材料外,BEI Resources 还鼓励和支持研究人员和机构的材料存放。使用 BEI Resources存放材料对研究人员和研究社区有许多优势,包括安全存储、社区访问和分发;同时保护存款人的知识产权。只要有需要,BEI 资源库将作为研究人员的资源进行维护。您在 BEI Resources 的存款是一项有助于未来研究的长期投资。
BEI Resources 自 2003 年起由美国典型培养物保藏中心 (ATCC) 根据合同管理。2016 年 5 月, ATCC获得了一份为期七年的继续管理 BEI Resources的合同。合同范围已扩大到更全面的研究目录材料,包括由其他政府支持的研究项目存放的材料,将提供给生物防御和新兴传染病科学界。真菌、寄生虫、载体和其他相关材料已添加到现有的细菌、病毒和毒素试剂中,涵盖 NIAID A、B 和 C 类优先病原体和 NIAID 指定的新发传染病病原体和生物。
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