产品图片
货号/SKU
NR-53818
货号/规格
EA
库存与交货期
3-8周
人民币价格
14000
试剂海关审批
A/B级风险物质只能直接使用人购买并持有实验室有效资质，其它询客服确认
国外采购
支持/部分限制一年内购买数量
厂牌
BEI Resources(ATCC)
产品基础信息
生物安全等级建议分类：美国、2
产品描述信息
NR-53818??SARS-Related Coronavirus 2, Wuhan-Hu-1 Spike-Pseudotyped Lentivirus, Luc2/ZsGreen(Pseudotyped Lentivirus)|SARS-Related Coronavirus 2|Wuhan-Hu-1 Spike-Pseudotyped Lentivirus, Luc2/ZsGreen| -60°C or colder|J Bloom, A BalaszAcknowledgment for publications should read “The following reagent was obtained through BEI Resources, NIAID, NIH: SARS-Related Coronavirus 2, Wuhan-Hu-1 Spike-Pseudotyped Lentivirus, Luc2/ZsGreen, NR-53818.”|Quantity limit per order for this item is 1. This item can be ordered twice a year. Orders over this limit will be sent to NIAID for approval before shipment.
A pseudotyped lentiviral form of the spike (S) glycoprotein gene from severe acute respiratory syndrome-related coronavirus 2 (SARS-CoV-2), Wuhan-Hu-1 (GenBank: NC_045512) was produced in human embryonic kidney HEK293 cells and purified by sucrose cushion. NR-53818 expresses a C-terminally truncated S glycoprotein, which increases titers of viral particles pseudotyped with SARS-CoV-2 S glycoprotein, as well as synthetic firefly luciferase (Luc2) and synthetic Zoanthus sp. green fluorescent protein (ZsGreen1). NR-53818 is produced from a plasmid kit (BEI Resources NR-52516, NR-52517, NR-52518, NR-52519 and NR-53742; kit NR-53816). Protocols for the use of these items are published, and updates are available at protocols.io.
The S glycoprotein mediates viral binding to the host angiotensin converting enzyme 2 (ACE2). This protein forms a trimer, and when bound to a host receptor, allows fusion of the viral and cellular membranes. The S protein is a target for neutralizing antibodies.
Successful use of this product necessarily depends on third party reagents. It is critically important that users confirm effective assay performance in their own laboratories using the appropriate control templates.
Each vial contains approximately 25 ?L of purified lentiviral particles from transfected HEK293 supernatant in DMEM supplemented with 10% heat-inactivated fetal bovine serum. NR-53818 includes sufficient material for one 96 well plate neutralization assay.
Additional information and tools are available at ViPR (Virus Pathogen Resource).
ZsGreen
For information on purchasing a license to use this product for research (for-profit entities) or commercial purposes (any entity) other than those permitted in the limited use label license, please contact a licensing representative at Takara Bio USA, Inc., by phone at 650.919.7320 or by e-mail at licensing@takarabio.com.
Luc2
Researchers may use this product for research use only, no commercial use is allowed. "Commercial use" means any and all uses of this product and derivatives by a party for money or other consideration and may include but is not limited to use in: (1) product manufacture; and (2) to provide a service, information or data; and/or resale of the product or its derivatives, whether or not such product or derivatives are resold for use in research. Researchers shall have no right to modify or otherwise create variations of the nucleotide sequence of the luciferase gene except that researchers may: (1) create fused gene sequences provided that the coding sequence of the resulting luciferase gene has no more than four deoxynucleotides missing at the affected terminus compared to the intact luciferase gene sequence, and (2) insert and remove nucleic acid sequences in splicing research predicated on the inactivation or reconstitution of the luminescence of the encoded luciferase. No other use or transfer of this product or derivatives is authorized without the prior express written consent of Promega. In addition, researchers must either: (1) use luminescent assay reagents purchased from Promega for all determinations of luminescence activity of this product and its derivatives; or (2) contact Promega to obtain a license for use of the product and its derivatives. Researchers may transfer derivatives to others for research use provided that at the time of transfer a copy of this label license is given to the recipients and recipients agree to be bound by the terms of this label license. With respect to any uses outside this label license, including any diagnostic, therapeutic or prophylactic uses, please contact Promega for supply and licensing information. PROMEGA MAKES NO REPRESENTATIONS OR WARRANTIES OF ANY KIND, EITHER EXPRESSED OR IMPLIED, INCLUDING FOR MERCHANTABILITY OR FITNESS FOR A PARTICULAR PURPOSE WITH REGARDS TO THE PRODUCT. The terms of this label license shall be governed under the laws of the State of Wisconsin, USA. This label license relates to Promega patents and/or patent applications on improvements to the luciferase gene.
A pseudotyped lentiviral form of the spike (S) glycoprotein gene from severe acute respiratory syndrome-related coronavirus 2 (SARS-CoV-2), Wuhan-Hu-1 (GenBank: NC_045512) was produced in human embryonic kidney HEK293 cells and purified by sucrose cushion. NR-53818 expresses a C-terminally truncated S glycoprotein, which increases titers of viral particles pseudotyped with SARS-CoV-2 S glycoprotein, as well as synthetic firefly luciferase (Luc2) and synthetic Zoanthus sp. green fluorescent protein (ZsGreen1). NR-53818 is produced from a plasmid kit (BEI Resources NR-52516, NR-52517, NR-52518, NR-52519 and NR-53742; kit NR-53816). Protocols for the use of these items are published, and updates are available at protocols.io.
The S glycoprotein mediates viral binding to the host angiotensin converting enzyme 2 (ACE2). This protein forms a trimer, and when bound to a host receptor, allows fusion of the viral and cellular membranes. The S protein is a target for neutralizing antibodies.
Successful use of this product necessarily depends on third party reagents. It is critically important that users confirm effective assay performance in their own laboratories using the appropriate control templates.
Each vial contains approximately 25 ?L of purified lentiviral particles from transfected HEK293 supernatant in DMEM supplemented with 10% heat-inactivated fetal bovine serum. NR-53818 includes sufficient material for one 96 well plate neutralization assay.
Additional information and tools are available at ViPR (Virus Pathogen Resource).
ZsGreen
For information on purchasing a license to use this product for research (for-profit entities) or commercial purposes (any entity) other than those permitted in the limited use label license, please contact a licensing representative at Takara Bio USA, Inc., by phone at 650.919.7320 or by e-mail at licensing@takarabio.com.
Luc2
Researchers may use this product for research use only, no commercial use is allowed. "Commercial use" means any and all uses of this product and derivatives by a party for money or other consideration and may include but is not limited to use in: (1) product manufacture; and (2) to provide a service, information or data; and/or resale of the product or its derivatives, whether or not such product or derivatives are resold for use in research. Researchers shall have no right to modify or otherwise create variations of the nucleotide sequence of the luciferase gene except that researchers may: (1) create fused gene sequences provided that the coding sequence of the resulting luciferase gene has no more than four deoxynucleotides missing at the affected terminus compared to the intact luciferase gene sequence, and (2) insert and remove nucleic acid sequences in splicing research predicated on the inactivation or reconstitution of the luminescence of the encoded luciferase. No other use or transfer of this product or derivatives is authorized without the prior express written consent of Promega. In addition, researchers must either: (1) use luminescent assay reagents purchased from Promega for all determinations of luminescence activity of this product and its derivatives; or (2) contact Promega to obtain a license for use of the product and its derivatives. Researchers may transfer derivatives to others for research use provided that at the time of transfer a copy of this label license is given to the recipients and recipients agree to be bound by the terms of this label license. With respect to any uses outside this label license, including any diagnostic, therapeutic or prophylactic uses, please contact Promega for supply and licensing information. PROMEGA MAKES NO REPRESENTATIONS OR WARRANTIES OF ANY KIND, EITHER EXPRESSED OR IMPLIED, INCLUDING FOR MERCHANTABILITY OR FITNESS FOR A PARTICULAR PURPOSE WITH REGARDS TO THE PRODUCT. The terms of this label license shall be governed under the laws of the State of Wisconsin, USA. This label license relates to Promega patents and/or patent applications on improvements to the luciferase gene.
主要内容
此项目的每个订单数量限制为1.此商品每年可订购两次.此限制的订单将在发货前发送到NIAID批准.
来自严重急性呼吸综合征相关的冠状病毒2（SARS-COV-2），武汉-U-1（Genbank：）在人胚胎肾Hek293细胞中产生并通过蔗糖垫纯化. NR-53818表达C末端截短的糖蛋白，其用SARS-COV-2S糖蛋白和合成萤火虫荧光素酶（Luc2）和合成的 Zoanthus Sp增加了序列序列的病毒颗粒的滴度.绿色荧光蛋白（Zsgreen1）. NR-53818由质粒试剂盒（BEI资源NR-52516，NR-52517，NR-52518，NR-52519和NR-53742; KIT NR-53816）产生.发布用于使用这些项目的协议，并且更新在协议.
S糖蛋白介导与宿主血管紧张素转化酶2（ACE2）的病毒结合.该蛋白质形成三聚体，并且当与宿主受体结合时，允许融合病毒和细胞膜. S蛋白是中和抗体的靶标.
成功使用本产品必然取决于第三方试剂.非常重要的是，用户使用适当的控制模板确认自己的实验室中有效的测定性能.
每个小瓶含有大约25μl纯化的慢病毒颗粒，其在补充有10％热灭活的胎牛血清的DMEM中的转染HEK293上清液中. NR-53818包括一个96个孔板中和测定的足够材料.
vipr （病毒病原体资源）提供了附加信息和工具.
Zsgreen
有关购买许可证用于使用本产品进行研究（营利性实体）或商业目的（任何实体）以外的商业目的（任何实体），请联系Takara Bio USA，Inc.的许可代表，电话在650.919.7320或通过在 licensing@takarabio.com .
Luc2
研究人员可以使用本产品仅供研究，不允许商业用途. “商业用途”是指通过派对或其他代价的缔约方的任何和所有用途，并可能包括但不限于：（1）产品制造; （2）提供服务，信息或数据;和/或转售产品或其衍生物，是否这些产品或衍生物是用于研究的转售.研究人员对荧光素酶基因的核苷酸序列的核苷酸序列的含量没有权利，除了研究人员可以：（1）产生融合基因序列，条件是所得荧光素酶基因的编码序列不超过四个脱氧核苷酸缺失与完整的荧光素酶基因序列相比，受影响的末端，（2）插入并除去剪接研究中的核酸序列，其预先灭活或重构编码的荧光素酶的发光的灭活或重构.未经Promega事先明确书面同意，否则没有其他使用或转移本产品或衍生物.此外，研究人员必须：（1）使用从Promega购买的发光测定试剂用于本产品的发光活动的所有测定及其衍生物;或（2）联系Promega以获得使用产品及其衍生物的许可证.研究人员可以将衍生品传递给其他人进行研究用途，条件规定，在转移此标签许可证的副本时，给予收件人，收件人同意受此标签许可条款的约束.关于此标签许可外的任何用途，包括任何诊断，治疗或预防用途，请联系Promega以供提供和许可信息. Promega没有任何形式的陈述或保证，无论是表达还是暗示，包括用于产品的特定目的的商家或适应性.该标签许可的条款应根据美国威斯康辛州的法律管辖.该标签许可证涉及Promega Patents和/或专利申请改善Luciferase基因.
厂牌介绍
BEI Resources 由美国国家过敏和传染病研究所 ( NIAID )成立，旨在为研究 A、B 和 C 类优先病原体、新兴传染病病原体、非病原微生物和其他相关微生物材料提供试剂、工具和信息到研究界。BEI Resources 获取、验证和生产科学家进行基础研究和开发改进的诊断测试、疫苗和疗法所需的试剂。通过将这些功能集中在 BEI Resources 中，可以监控科学界对这些材料的访问和使用，并确保试剂的质量控制。
除了为传染病界提供材料外，BEI Resources 还鼓励和支持研究人员和机构的材料存放。使用 BEI Resources存放材料对研究人员和研究社区有许多优势，包括安全存储、社区访问和分发；同时保护存款人的知识产权。只要有需要，BEI 资源库将作为研究人员的资源进行维护。您在 BEI Resources 的存款是一项有助于未来研究的长期投资。
BEI Resources 自 2003 年起由美国典型培养物保藏中心 (ATCC) 根据合同管理。2016 年 5 月， ATCC获得了一份为期七年的继续管理 BEI Resources的合同。合同范围已扩大到更全面的研究目录材料，包括由其他政府支持的研究项目存放的材料，将提供给生物防御和新兴传染病科学界。真菌、寄生虫、载体和其他相关材料已添加到现有的细菌、病毒和毒素试剂中，涵盖 NIAID A、B 和 C 类优先病原体和 NIAID 指定的新发传染病病原体和生物。
品牌标识
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