NR-53796单克隆抗SARS相关冠状病毒2SpikeRBD-mFc融合蛋白(体外生产)(单克隆抗体）

NR-53796?? Monoclonal Anti-SARS-Related Coronavirus 2 Spike RBD-mFc Fusion Protein (produced in vitro)(Monoclonal Antibodies)|SARS-Related Coronavirus 2|Monoclonal Anti-SARS-Related Coronavirus 2 Spike RBD-mFc Fusion Protein (produced in vitro)| -20°C to -80°C|Sino BiologicalAcknowledgment for publications should read “The following reagent was obtained through BEI Resources, NIAID, NIH: Monoclonal Anti-SARS-Related Coronavirus 2 Spike RBD-mFc Fusion Protein (produced in vitro), NR-53796.”|Quantity limit per order for this item is 1. This item can be ordered twice a year. Orders over this limit will be sent to NIAID for approval before shipment. Antibody Class: IgG2b Mouse monoclonal antibody prepared against the fusion protein containing severe acute respiratory syndrome-related coronavirus 2 (SARS-CoV-2) spike (S) glycoprotein receptor binding domain (RBD) (R319 to F541) and mouse IgG Fc (mFc) domain was purified from a hybridoma supernatant by protein A affinity chromatography. The B cell hybridoma was generated by the fusion of mouse myeloma cells with splenocytes from mice immunized with recombinant SARS-CoV-2 spike RBD-mFc fusion protein (Sino Biological 40592-V05H; GenPept: YP_009724390.1). NR-53796 is specific to the SARS-CoV-2 spike RBD as shown in ELISA, with cross reactivity to the SARS-CoV-2 spike S1 protein (Sino Biological 40591-V08B1). No cross reactivity was observed in ELISA with S1 glycoproteins from SARS-CoV, MERS-CoV, HCoV-HKU1 (isolates N1 and N5), HCoV-NL63, HCoV-229E and HCoV-OC43. The biological activity of NR-53796 was measured by its binding ability using biosensor analysis, in which biotinylated recombinant SARS-CoV-2 Spike RBD recombinant protein (His tag) (Sino Biological 40592-V08B-B) can bind NR-53796; the affinity constant is 0.05 to 0.07 nM. Inhibitor Screening ELISA Kit (Sino Biological KIT001) detected serial dilutions of NR-53796; the IC₅₀ is 3.694 nM. NR-53796 is functional in in vitro microneutralization assays using 293T-ACE2 cells infected with SARS-CoV-2 spike pseudovirus; the IC₅₀ is 0.41 micrograms per milliliter. Each vial of NR-53796 contains approximately 50 ?g of purified monoclonal antibody in phosphate buffered saline (PBS). For more information please visit the Sino Biological website.