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产品图片
货号/SKU
NR-53522
货号/规格
EA
库存与交货期
3-8周
人民币价格
14000
试剂海关审批
A/B级风险物质只能直接使用人购买并持有实验室有效资质，其它询客服确认
国外采购
支持/部分限制一年内购买数量
厂牌
BEI Resources(ATCC)
产品基础信息
生物安全等级建议分类：美国、1
产品描述信息
NR-53522??Homo sapiens, Human Lung Carcinoma Cells (A549) Expressing Human Angiotensin-Converting Enzyme 2 (HA-FLAG)(Cell Banks)|Homo sapiens|Human Lung Carcinoma Cells (A549) Expressing Human Angiotensin-Converting Enzyme 2 (HA-FLAG)|-100°C or colder, preferably in the vapor phase of a liquid
nitrogen freezer|A García-SastreAcknowledgment for publications should read "The following reagent was obtained through BEI Resources, NIAID, NIH: Human Lung Carcinoma Cells (A549) Expressing Human Angiotensin-Converting Enzyme 2 (HA-FLAG), NR-53522."|Quantity limit per order for this item is 1. This item can be ordered twice a year. Orders over this limit will be sent to NIAID for approval before shipment.
This item is currently in our production queue. Please allow ample time for distribution lots to be made available.
NR-53522 contains a preparation of human (Homo sapiens) lung carcinoma epithelial cells (A549) expressing human angiotensin-converting enzyme 2 (ACE2) with C-terminal HA-tag and FLAG-tag. A549-hACE2 (HA-FLAG) cells were created by transducing A549 cells with lentiviral vector pLVX-Puro (Clontech?) expressing human ACE2 under the control of human cytomegalovirus immediate early promoter. A549-hACE2 (HA-FLAG) cells have been shown to express ACE2 at high levels. pLVX-Puro contains a puromycin resistance gene for maintenance of stable transductants.
Note: NR-53522 is not a clonal population. If a clonal population is required for subsequent work, user should first select a stable clone from the provided mixed cell population. It is recommended that the cells undergo minimal passages.
ACE2 is a human receptor that is expressed widely, including in heart, kidney, small intestine and lung cells, and is involved in the regulation of hypertension and other cardiovascular diseases. The SARS-Related Coronavirus 2 spike glycoprotein mediates viral binding to the host ACE2 receptor. This protein forms a trimer, and when bound to ACE2, allows fusion of the viral and cellular membranes, allowing viral entry and replication. The interaction of ACE2 and the spike glycoprotein during viral infection is currently under study.
Each vial contains approximately 1 mL of cell culture suspension frozen in Dulbecco’s Modified Eagle’s Medium (80%) containing fetal bovine serum (10%) and DMSO (10%) cryopreservative. Sufficient cells are provided to initiate at least one new culture.
This item is currently in our production queue. Please allow ample time for distribution lots to be made available.
NR-53522 contains a preparation of human (Homo sapiens) lung carcinoma epithelial cells (A549) expressing human angiotensin-converting enzyme 2 (ACE2) with C-terminal HA-tag and FLAG-tag. A549-hACE2 (HA-FLAG) cells were created by transducing A549 cells with lentiviral vector pLVX-Puro (Clontech?) expressing human ACE2 under the control of human cytomegalovirus immediate early promoter. A549-hACE2 (HA-FLAG) cells have been shown to express ACE2 at high levels. pLVX-Puro contains a puromycin resistance gene for maintenance of stable transductants.
Note: NR-53522 is not a clonal population. If a clonal population is required for subsequent work, user should first select a stable clone from the provided mixed cell population. It is recommended that the cells undergo minimal passages.
ACE2 is a human receptor that is expressed widely, including in heart, kidney, small intestine and lung cells, and is involved in the regulation of hypertension and other cardiovascular diseases. The SARS-Related Coronavirus 2 spike glycoprotein mediates viral binding to the host ACE2 receptor. This protein forms a trimer, and when bound to ACE2, allows fusion of the viral and cellular membranes, allowing viral entry and replication. The interaction of ACE2 and the spike glycoprotein during viral infection is currently under study.
Each vial contains approximately 1 mL of cell culture suspension frozen in Dulbecco’s Modified Eagle’s Medium (80%) containing fetal bovine serum (10%) and DMSO (10%) cryopreservative. Sufficient cells are provided to initiate at least one new culture.
主要内容
此项目的每个订单数量限制为1.此商品每年可订购两次.通过此限制的订单将在发货前发送至NIAID进行批准.
此项目目前处于我们的生产队列中.请允许充足的时间进行分销批次.
NR-53522含有用C-末端HA标签和Flag标签表达人血管紧张素转换酶2（ACE2）的人类（HOMO sapiens）肺癌上皮细胞（A549）的制备.通过在人巨细胞病毒立即早期启动子的控制下将A549细胞转化为表达人ACE2的慢病毒载体PLVX-plvx-plvx-puro（clontech?）来产生A549-Hace2（Ha-Flag）细胞.已显示A549-Hace2（HA-FLAG）细胞在高水平以ACE2表达. plvx-puro含有嘌呤霉素抗性基因，用于维持稳定的转鼓.
注意：NR-53522不是克隆人群.如果后续工作所需的克隆人群，则用户应该首先从提供的混合细胞群中选择稳定的克隆.建议细胞接受最小的段落.
ACE2是一种人类受体，广泛表达，包括心脏，肾，小肠和肺细胞，并参与高血压和其他心血管疾病的调节. SARS相关的冠状病毒2穗糖蛋白介导病毒结合到宿主ACE2受体.该蛋白质形成三聚体，并且当与ACE2结合时，允许融合病毒和细胞膜，允许病毒进入和复制.在病毒感染期间ACE2和穗糖蛋白的相互作用目前正在研究中.
每个小瓶含有大约1ml的含有胎儿牛血清（10％）和DMSO（10％）冷冻保存的胎儿牛血清（80％）的细胞培养悬浮液.提供足够的细胞以引发至少一种新培养物.
厂牌介绍
BEI Resources 由美国国家过敏和传染病研究所 ( NIAID )成立，旨在为研究 A、B 和 C 类优先病原体、新兴传染病病原体、非病原微生物和其他相关微生物材料提供试剂、工具和信息到研究界。BEI Resources 获取、验证和生产科学家进行基础研究和开发改进的诊断测试、疫苗和疗法所需的试剂。通过将这些功能集中在 BEI Resources 中，可以监控科学界对这些材料的访问和使用，并确保试剂的质量控制。
除了为传染病界提供材料外，BEI Resources 还鼓励和支持研究人员和机构的材料存放。使用 BEI Resources存放材料对研究人员和研究社区有许多优势，包括安全存储、社区访问和分发；同时保护存款人的知识产权。只要有需要，BEI 资源库将作为研究人员的资源进行维护。您在 BEI Resources 的存款是一项有助于未来研究的长期投资。
BEI Resources 自 2003 年起由美国典型培养物保藏中心 (ATCC) 根据合同管理。2016 年 5 月， ATCC获得了一份为期七年的继续管理 BEI Resources的合同。合同范围已扩大到更全面的研究目录材料，包括由其他政府支持的研究项目存放的材料，将提供给生物防御和新兴传染病科学界。真菌、寄生虫、载体和其他相关材料已添加到现有的细菌、病毒和毒素试剂中，涵盖 NIAID A、B 和 C 类优先病原体和 NIAID 指定的新发传染病病原体和生物。
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