产品图片
货号/SKU
NR-52421
货号/规格
EA
库存与交货期
3-8周
人民币价格
14000
试剂海关审批
A/B级风险物质只能直接使用人购买并持有实验室有效资质，其它询客服确认
国外采购
支持/部分限制一年内购买数量
厂牌
BEI Resources(ATCC)
产品基础信息
生物安全等级建议分类：美国、1
产品描述信息
NR-52421?? Vector pCMV Containing the SARS-Related Coronavirus 2, Wuhan-Hu-1 Spike Glycoprotein Ectodomain(Plasmid/Vectors)|SARS-Related Coronavirus 2|Vector pCMV Containing the SARS-Related Coronavirus 2, Wuhan-Hu-1 Spike Glycoprotein Ectodomain| -20°C or colder|D VeeslerAcknowledgment for publications should read “The following reagent was contributed by David Veesler for distribution through BEI Resources, NIAID, NIH: Vector pCMV Containing the SARS-Related Coronavirus 2, Wuhan-Hu-1 Spike Glycoprotein Ectodomain, NR-52421. Work making use of this reagent should also cite Walls, A. C. and Y.-J. Park, et al. Cell 181 (2020): 281-292. PubMed: 32155444.”|Quantity limit per order for this item is 1. This item can be ordered twice a year. Orders over this limit will be sent to NIAID for approval before shipment.
Note: The label on the vial is incorrect; the correct vector is pCMV. The pCMV vector is identical to the pcDNA3.1 vector used for other plasmids provided by Dr. Veesler.
The vector for the spike (S) glycoprotein gene from severe acute respiratory syndrome-related coronavirus 2 (SARS-CoV-2), Wuhan-Hu-1 (GenBank: MN908947) was designed by codon optimizing the S glycoprotein ectodomain (residues 14 to 1211) for mammalian expression, fused to an N-terminal mu-phosphatase signal sequence and C-terminal trimerizing foldon domain and octa-histidine tag, and subcloned into the pCMV mammalian expression vector. The recombinant protein is stabilized by substitution at the furin S1/S2 cleavage site (RRAR→SGAG; residues 682 to 685) and KV→PP mutations (residues 983 and 984). NR-52421 contains the beta-lactamase gene, TEM-116, to provide transformant selection through ampicillin resistance in Escherichia coli (E. coli), and a neomycin (G418) selectable marker for mammalian expression. The resulting size of the plasmid is approximately 9300 base pairs. The complete plasmid sequence and map are provided on the BEI Resources webpage. The plasmid was produced in E. coli and extracted.
The S glycoprotein mediates viral binding to the host angiotensin converting enzyme 2 (ACE2). This protein forms a trimer, and when bound to a host receptor allows fusion of the viral and cellular membranes. The S protein is a target for neutralizing antibodies.
Each vial contains 0.3 ?g of plasmid DNA in 10 mM Tris-HCl, 1 mM EDTA, pH 8.0. The vial should be centrifuged prior to opening. Note: The contents of the vial should be used to replicate the plasmid in E. coli prior to mammalian expression.
Note: The label on the vial is incorrect; the correct vector is pCMV. The pCMV vector is identical to the pcDNA3.1 vector used for other plasmids provided by Dr. Veesler.
The vector for the spike (S) glycoprotein gene from severe acute respiratory syndrome-related coronavirus 2 (SARS-CoV-2), Wuhan-Hu-1 (GenBank: MN908947) was designed by codon optimizing the S glycoprotein ectodomain (residues 14 to 1211) for mammalian expression, fused to an N-terminal mu-phosphatase signal sequence and C-terminal trimerizing foldon domain and octa-histidine tag, and subcloned into the pCMV mammalian expression vector. The recombinant protein is stabilized by substitution at the furin S1/S2 cleavage site (RRAR→SGAG; residues 682 to 685) and KV→PP mutations (residues 983 and 984). NR-52421 contains the beta-lactamase gene, TEM-116, to provide transformant selection through ampicillin resistance in Escherichia coli (E. coli), and a neomycin (G418) selectable marker for mammalian expression. The resulting size of the plasmid is approximately 9300 base pairs. The complete plasmid sequence and map are provided on the BEI Resources webpage. The plasmid was produced in E. coli and extracted.
The S glycoprotein mediates viral binding to the host angiotensin converting enzyme 2 (ACE2). This protein forms a trimer, and when bound to a host receptor allows fusion of the viral and cellular membranes. The S protein is a target for neutralizing antibodies.
Each vial contains 0.3 ?g of plasmid DNA in 10 mM Tris-HCl, 1 mM EDTA, pH 8.0. The vial should be centrifuged prior to opening. Note: The contents of the vial should be used to replicate the plasmid in E. coli prior to mammalian expression.
主要内容
此项目的每个订单数量限制为1.此商品每年可订购两次.通过此限制的订单将在发货前发送至NIAID进行批准.
注意：小瓶上的标签不正确;正确的矢量是PCMV . PCMV载体与用于vesler博士提供的其他质粒的PCDNA3.1载体相同.
来自严重急性呼吸综合征相关的冠状病毒2（SARS-COV-2），武汉-U-1（Genbank：结果由密码子优化用于哺乳动物表达的S糖蛋白外立骨髓（残留物14至1211），融合到N-末端MU-磷酸酶信号序列和C-终端三聚集卷轴域和Octa-hasteatine标签，并括到 pcmv 哺乳动物表达式矢量.通过在Furin S1 / S2切割位点（RRAR→SGAG;残基682至685）和KV→PP突变（残基983和984）稳定重组蛋白质通过取代稳定. NR-52421含有β-内酰胺酶基因TEM-116，以通过在哺乳动物表达中通过氨苄青霉素抗性提供转化体选择，以及用于哺乳动物表达的新霉素（G418）选择标记.得到的质粒尺寸约为9300碱基对.在北部资源网页上提供完整的质粒序列和地图.在 e中制备质粒. Coli 并提取.
S糖蛋白介导与宿主血管紧张素转化酶2（ACE2）的病毒结合.该蛋白质形成三聚体，并且当与宿主受体结合时，允许融合病毒和细胞膜. S蛋白是中和抗体的靶标.
每个小瓶含有0.3μg质粒DNA，10mM Tris-HCl，1mM EDTA，pH8.0.在打开之前应该离心小瓶. 注意：Vial的内容应该用于复制 e中的质粒. Coli 在哺乳动物表达之前.
厂牌介绍
BEI Resources 由美国国家过敏和传染病研究所 ( NIAID )成立，旨在为研究 A、B 和 C 类优先病原体、新兴传染病病原体、非病原微生物和其他相关微生物材料提供试剂、工具和信息到研究界。BEI Resources 获取、验证和生产科学家进行基础研究和开发改进的诊断测试、疫苗和疗法所需的试剂。通过将这些功能集中在 BEI Resources 中，可以监控科学界对这些材料的访问和使用，并确保试剂的质量控制。
除了为传染病界提供材料外，BEI Resources 还鼓励和支持研究人员和机构的材料存放。使用 BEI Resources存放材料对研究人员和研究社区有许多优势，包括安全存储、社区访问和分发；同时保护存款人的知识产权。只要有需要，BEI 资源库将作为研究人员的资源进行维护。您在 BEI Resources 的存款是一项有助于未来研究的长期投资。
BEI Resources 自 2003 年起由美国典型培养物保藏中心 (ATCC) 根据合同管理。2016 年 5 月， ATCC获得了一份为期七年的继续管理 BEI Resources的合同。合同范围已扩大到更全面的研究目录材料，包括由其他政府支持的研究项目存放的材料，将提供给生物防御和新兴传染病科学界。真菌、寄生虫、载体和其他相关材料已添加到现有的细菌、病毒和毒素试剂中，涵盖 NIAID A、B 和 C 类优先病原体和 NIAID 指定的新发传染病病原体和生物。
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