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产品图片
货号/SKU
NR-50174
货号/规格
EA
库存与交货期
3-8周
人民币价格
14000
试剂海关审批
A/B级风险物质只能直接使用人购买并持有实验室有效资质,其它询客服确认
国外采购
支持/部分限制一年内购买数量
厂牌
BEI Resources(ATCC)
产品基础信息
生物安全等级建议分类:美国、1
产品描述信息
NR-50174?? H5 Hemagglutinin (HA) Protein from Influenza Virus, A/northern Pintail/WA/40964 /2014 (H5N8), Recombinant from Baculovirus(Proteins)|Influenza A virus|H5 Hemagglutinin (HA) Protein from Influenza Virus, A/northern Pintail/WA/40964 /2014 (H5N8), Recombinant from Baculovirus|-20°C|F KrammerAcknowledgment for publications should read “The following reagent was obtained through BEI Resources, NIAID, NIH: H5 Hemagglutinin (HA) Protein from Influenza Virus, A/northern Pintail/WA/40964/2014 (H5N8), Recombinant from Baculovirus, NR-50174.”|Quantity limit per order for this item is 1. This item can be ordered twice a year. Orders over this limit will be sent to NIAID for approval before shipment.
A recombinant form of the H5 hemagglutinin (HA) protein from influenza A virus, A/northern Pintail/WA/40964/2014 (H5N8) was produced in Sf9 insect cells using a baculovirus expression vector system. The predicted transmembrane and endodomain coding regions of the HA gene were replaced with a synthetic gene segment encoding a thrombin cleavage site, trimerizing (foldon) domain and eight histidine residues, as described for several influenza A virus subtypes. The HA0 form of the protein was purified by nickel affinity chromatography and then treated with thrombin to remove the foldon domain and the histidine tag. The thrombin-treated protein was further purified prior to final formulation.
Each vial contains 50 ?g to 150 ?g of purified recombinant HA protein in PBS (pH 7.4).
A recombinant form of the H5 hemagglutinin (HA) protein from influenza A virus, A/northern Pintail/WA/40964/2014 (H5N8) was produced in Sf9 insect cells using a baculovirus expression vector system. The predicted transmembrane and endodomain coding regions of the HA gene were replaced with a synthetic gene segment encoding a thrombin cleavage site, trimerizing (foldon) domain and eight histidine residues, as described for several influenza A virus subtypes. The HA0 form of the protein was purified by nickel affinity chromatography and then treated with thrombin to remove the foldon domain and the histidine tag. The thrombin-treated protein was further purified prior to final formulation.
Each vial contains 50 ?g to 150 ?g of purified recombinant HA protein in PBS (pH 7.4).
主要内容
此项目的每个订单数量限制为1.此商品每年可订购两次.通过此限制的订单将在发货前发送到NIAID进行批准. H5 Hemagglutinin(HA)的重组形式
来自流感的蛋白质是病毒,A /北九尾/ WA / 40964/2014(H5N8)使用杆状病毒表达载体系统在SF9昆虫细胞中产生.预测的跨膜和内阳瘤
HA基因的编码区域用合成基因段代替
编码凝血酶裂解位点,三聚化(粘合剂)结构域和八个组氨酸
残留物,如几种流感病毒亚型所描述的.蛋白质的HA0形式纯化
镍亲和层析,然后用凝血酶处理以除去
卷轴域和组氨酸标签.这
在最终制剂之前进一步纯化凝血酶处理的蛋白质. 每个小瓶含有 50μg到 150μgpbs中的纯化重组ha蛋白(pH7.4).
厂牌介绍
BEI Resources 由美国国家过敏和传染病研究所 ( NIAID )成立,旨在为研究 A、B 和 C 类优先病原体、新兴传染病病原体、非病原微生物和其他相关微生物材料提供试剂、工具和信息到研究界。BEI Resources 获取、验证和生产科学家进行基础研究和开发改进的诊断测试、疫苗和疗法所需的试剂。通过将这些功能集中在 BEI Resources 中,可以监控科学界对这些材料的访问和使用,并确保试剂的质量控制。
除了为传染病界提供材料外,BEI Resources 还鼓励和支持研究人员和机构的材料存放。使用 BEI Resources存放材料对研究人员和研究社区有许多优势,包括安全存储、社区访问和分发;同时保护存款人的知识产权。只要有需要,BEI 资源库将作为研究人员的资源进行维护。您在 BEI Resources 的存款是一项有助于未来研究的长期投资。
BEI Resources 自 2003 年起由美国典型培养物保藏中心 (ATCC) 根据合同管理。2016 年 5 月, ATCC获得了一份为期七年的继续管理 BEI Resources的合同。合同范围已扩大到更全面的研究目录材料,包括由其他政府支持的研究项目存放的材料,将提供给生物防御和新兴传染病科学界。真菌、寄生虫、载体和其他相关材料已添加到现有的细菌、病毒和毒素试剂中,涵盖 NIAID A、B 和 C 类优先病原体和 NIAID 指定的新发传染病病原体和生物。
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