NR-49335刚地弓形虫,PruA7Δhxgprt::gra2-GFP::tub1-FLUC(寄生原生动物）

NR-49335??Toxoplasma gondii, Pru A7 Δhxgprt::gra2-GFP::tub1-FLUC(Parasitic Protozoa)|Toxoplasma gondii|Pru A7 Δhxgprt::gra2-GFP::tub1-FLUC|-130°C or colder (Note: Do not under any circumstances store vials at temperatures warmer than -70°C. Storage under these conditions will result in the death of the culture.)|J BoothroydAcknowledgment for publications should read "The following reagent was obtained through BEI Resources, NIAID, NIH: Toxoplasma gondii, Strain Pru A7 Δhxgprt::gra2-GFP::tub1-FLUC, NR-49335."|Quantity limit per order for this item is 1. This item can be ordered twice a year. Orders over this limit will be sent to NIAID for approval before shipment. This item is currently in our production queue. Please allow ample time for distribution lots to be made available. Toxoplasma gondii (T. gondii), strain Pru A7 Δhxgprt::gra2-GFP::tub1-FLUC (also referred to as Pru A7 Δhpt::gra2-GFP::tub1-FLUC) was deposited to BEI Resources as a green-fluorescent-protein- and luciferase-expressing transgenic clone derived from the deletion mutant strain Prugniaud (Pru) Δhxgprt (also referred to as Pru Δhpt), which lacks the hxgprt (hypoxanthine-xanthine-guanine-phosphoribosyltransferase) gene. The original parent strain Prugniaud (Pru) is a Type II strain originally isolated in 1964 from a human with lethal congenital toxoplasmosis in Limoges, France. T. gondii, strain Pru A7 Δhxgprt::gra2-GFP::tub1-FLUC was engineered by transfection of the Pru Δhxgprt strain with a plasmid expressing GFP and FLUC under the control of the Toxoplasma GRA2 and TUB1 promoters, respectively, resulting in constitutive expression of GFP and FLUC in both tachyzoite and bradyzoite stages of the parasite. Strain Pru A7 Δhxgprt::gra2 GFP::tub1-FLUC has been used to study the role of bradyzoite-specific antigens in establishing infections. Each vial of NR-49335 contains approximately 0.5 mL of culture in cryopreservative [7.5% dimethylsulfoxide (DMSO)].