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产品图片
货号/SKU
NR-44008
货号/规格
EA
库存与交货期
3-8周
人民币价格
14000
试剂海关审批
A/B级风险物质只能直接使用人购买并持有实验室有效资质，其它询客服确认
国外采购
支持/部分限制一年内购买数量
厂牌
BEI Resources(ATCC)
产品基础信息
生物安全等级建议分类：美国、3
产品描述信息
NR-44008??SARS coronavirus, Recombinant Infectious Mouse-Adapted Mutant of Urbani Strain with Non-Functional Exoribonuclease (icSARS-CoV-MA15-ExoN1)(Viruses)|SARS coronavirus|Recombinant Infectious Mouse-Adapted Mutant of Urbani Strain with Non-Functional Exoribonuclease (icSARS-CoV-MA15-ExoN1)|-60°C or colder|RS BaricAcknowledgment for publications should read "The following reagent was obtained through BEI Resources, NIAID, NIH: SARS Coronavirus, Recombinant Infectious Mouse-Adapted Mutant of Urbani Strain with Non-Functional Exoribonuclease (icSARS-CoV-MA15-ExoN1), NR-44008."|Quantity limit per order for this item is 1. This item can be ordered twice a year. Orders over this limit will be sent to NIAID for approval before shipment.
Recombinant infectious mouse-adapted mutant of Urbani strain with non-functional exoribonuclease (icSARS-CoV-MA15-ExoN1).
The complete genome of SARS-CoV, MA15 ExoN1 has been sequenced (GenBank: FJ882943).
NR-44008 is an engineered recombinant coronavirus based on a full-length infectious clone of the SARS-CoV, Urbani genome. SARS-CoV, Urbani was serially passaged in the lungs of BALB/c mice fifteen times, and a biological clone, MA15, was recovered which was 100% lethal in BALB/c mice within six days of inoculation. Alanine substitution mutations in the DEDD motif 1 of nonstructural protein 14 (nsp14) that inactivate the coronavirus 3'→5' exoribonuclease (ExoN) were subsequently engineered onto the MA15 background. The resulting virus, termed MA-ExoN (or MA15 ExoN1), expresses a stable mutator phenotype with profoundly decreased fidelity and attenuation of pathogenesis both in young and in aged immunosenescent mice. The ExoN inactivation genotype and mutator phenotype did not revert to virulence even after serial passage or long-term persistent infection in vivo. Vaccination with the live attenuated virus protected aged mice from lethal challenge with "wild type" mouse adapted SARS-CoV.
Each vial contains approximately 1 mL of cell lysate and supernatant from Vero E6 (C1008) cells (ATCC ? CRL-1586?) infected with icSARS-CoV-MA15-ExoN1.
Additional information and tools are available at ViPR (Virus Pathogen Resource).
Recombinant infectious mouse-adapted mutant of Urbani strain with non-functional exoribonuclease (icSARS-CoV-MA15-ExoN1).
The complete genome of SARS-CoV, MA15 ExoN1 has been sequenced (GenBank: FJ882943).
NR-44008 is an engineered recombinant coronavirus based on a full-length infectious clone of the SARS-CoV, Urbani genome. SARS-CoV, Urbani was serially passaged in the lungs of BALB/c mice fifteen times, and a biological clone, MA15, was recovered which was 100% lethal in BALB/c mice within six days of inoculation. Alanine substitution mutations in the DEDD motif 1 of nonstructural protein 14 (nsp14) that inactivate the coronavirus 3'→5' exoribonuclease (ExoN) were subsequently engineered onto the MA15 background. The resulting virus, termed MA-ExoN (or MA15 ExoN1), expresses a stable mutator phenotype with profoundly decreased fidelity and attenuation of pathogenesis both in young and in aged immunosenescent mice. The ExoN inactivation genotype and mutator phenotype did not revert to virulence even after serial passage or long-term persistent infection in vivo. Vaccination with the live attenuated virus protected aged mice from lethal challenge with "wild type" mouse adapted SARS-CoV.
Each vial contains approximately 1 mL of cell lysate and supernatant from Vero E6 (C1008) cells (ATCC ? CRL-1586?) infected with icSARS-CoV-MA15-ExoN1.
Additional information and tools are available at ViPR (Virus Pathogen Resource).
主要内容
此项目的每个订单数量限制为1.此项目每年可订购两次.通过此限制的订单将在发货前发送到NIAID进行批准. 与非功能性放射酶（ICSARS-COV-MA15-EXON1）的Urbombinant Invedious小鼠适应突变体（ICSARS-COV-MA15-EXON1）. SARS-COV的完整基因组，MA15 EXON1已被测序（Genbank：FJ882943）. NR-44008是基于SARS-COV的全长传染性克隆的工程重组冠状病毒，Urbani Genome. SARS-COV，Urbani在Balb / C小鼠的肺部连续传代，并且在接种的六天内回收了生物克隆，MA15，在BALB / C小鼠中致死100％.随后在MA15背景上工程，在非结构蛋白14（NSP14）的DIDD蛋白14（NSP14）的丙氨酸替代突变中灭活冠状病毒3'→5'AxORIBONUCLEASE（外显子）.所得病毒，称为Ma-Exon（或Ma15 Exon1），表达稳定的突变表型，其在年轻和老化的免疫小鼠中具有深刻地降低的保证性和发病机制的衰减.即使在串联通道或长期持续感染在体内，外显子灭活基因型和突变表型也没有恢复毒力.用活衰减病毒的疫苗接种受到致命挑战的致命攻击与“野生型”小鼠适应SARS-COV. 每个小瓶含有约1ml的细胞裂解物和来自Vero E6（C1008）细胞的上清液（ATCC ? CRL-1586?）感染ISSAR-COV-MA15-EXON1. vipr （病毒病原体资源）.
厂牌介绍
BEI Resources 由美国国家过敏和传染病研究所 ( NIAID )成立，旨在为研究 A、B 和 C 类优先病原体、新兴传染病病原体、非病原微生物和其他相关微生物材料提供试剂、工具和信息到研究界。BEI Resources 获取、验证和生产科学家进行基础研究和开发改进的诊断测试、疫苗和疗法所需的试剂。通过将这些功能集中在 BEI Resources 中，可以监控科学界对这些材料的访问和使用，并确保试剂的质量控制。
除了为传染病界提供材料外，BEI Resources 还鼓励和支持研究人员和机构的材料存放。使用 BEI Resources存放材料对研究人员和研究社区有许多优势，包括安全存储、社区访问和分发；同时保护存款人的知识产权。只要有需要，BEI 资源库将作为研究人员的资源进行维护。您在 BEI Resources 的存款是一项有助于未来研究的长期投资。
BEI Resources 自 2003 年起由美国典型培养物保藏中心 (ATCC) 根据合同管理。2016 年 5 月， ATCC获得了一份为期七年的继续管理 BEI Resources的合同。合同范围已扩大到更全面的研究目录材料，包括由其他政府支持的研究项目存放的材料，将提供给生物防御和新兴传染病科学界。真菌、寄生虫、载体和其他相关材料已添加到现有的细菌、病毒和毒素试剂中，涵盖 NIAID A、B 和 C 类优先病原体和 NIAID 指定的新发传染病病原体和生物。
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