NR-42490主要利什曼原虫,NIHSD(MHOM/SN/74/SD),Δgp631-7+gp63-1(寄生原生动物）

NR-42490??Leishmania major, NIH SD (MHOM/SN/74/SD), Δgp63 1-7 + gp63-1(Parasitic Protozoa)|Leishmania major|NIH SD (MHOM/SN/74/SD), Δgp63 1-7 + gp63-1|-130°C or colder (Note: Do not under any circumstances store vials at temperatures warmer than -70°C. Storage under these conditions will result in the death of the culture.)|R McMasterAcknowledgment for publications should read "The following reagent was obtained through BEI Resources, NIAID, NIH: Leishmania major, Strain NIH SD (MHOM/SN/74/SD) Δgp63 1-7 + gp63-1, NR-42490."|Quantity limit per order for this item is 1. This item can be ordered twice a year. Orders over this limit will be sent to NIAID for approval before shipment.Leishmania major (L. major), strain NIH SD (MHOM/SN/74/SD) Δgp63 1-7 + gp63-1 [also referred to as (MHOM/SN/74/SD) Δgp63 1-7 + gp63-1 and NIH S (MHOM/SN/74/Seidman) Δgp63 1-7 + gp63-1] is a mutant of the wild-type strain NIH SD (MHOM/SN/74/SD) created by the targeted deletion of the leishmanolysin (gp63) gene complex followed by transfection with plasmid pLEXNeo-gp63-1 containing the gp63-1 gene. Complementation mutants were selected from G418-resistant promastigotes and are reported to be resistant to hygromycin B, nourseothricin and neomycin. The parent strain NIH SD (MHOM/SN/74/SD) [also known as strain NIH S (MHOM/SN/74/Seidman)] was isolated in 1973 from a human patient with cutaneous leishmaniasis in Senegal, West Africa.L. major, strain NIH SD (MHOM/SN/74/SD) Δgp63 1-7 is reported to display increased sensitivity to complement-mediated lysis and delayed lesion formation in a mouse model.Each vial of NR-42490 contains approximately 0.5 mL of culture in 10% glycerol.