产品图片
货号/SKU
NR-40624
货号/规格
EA
库存与交货期
3-8周
人民币价格
14000
试剂海关审批
A/B级风险物质只能直接使用人购买并持有实验室有效资质,其它询客服确认
国外采购
支持/部分限制一年内购买数量
厂牌
BEI Resources(ATCC)
产品基础信息
生物安全等级建议分类:美国、2
产品描述信息
NR-40624??Salmonella enterica subsp. enterica, 14028s ΔSTM14:2908 (Serovar Typhimurium) (Bacteria)|Salmonella enterica subsp. enterica|14028s ΔSTM14:2908 (Serovar Typhimurium) |-60°C or colder|F HeffronAcknowledgment for publications should read "The following reagent was obtained through BEI Resources, NIAID, NIH: Salmonella enterica subsp. enterica, Strain 14028s ΔSTM14:2908 (Serovar Typhimurium), NR-40624."|Quantity limit per order for this item is 1. This item can be ordered twice a year. Orders over this limit will be sent to NIAID for approval before shipment.
Salmonella enterica (S. enterica) subsp. enterica, strain 14028s ΔSTM14:2908 (serovar Typhimurium) was derived from strain 14028s (strain 14028s was originally known as strain 14028, however, a variant of the original strain with a rough colony morphology was designated 14028r and the original smooth strain was renamed 14028s). Strain 14028 is a descendent of strain CDC 6516-60 which was isolated from pools of hearts and livers of 4-week-old chickens.
The ΔSTM14:2908 mutant of strain 14028s was produced by creating a PCR product with STM14:2908 homologous sequences at the 5' and 3' ends of a linear fragment containing a kanamycin resistance cassette. S. enterica subsp. enterica, strain 14028s was transformed, and insertion of the kan cassette in place of STM14:2908 was confirmed by PCR. The final non-polar deletions were constructed by elimination of the kan cassette. The complete genome (GenBank: CP001363.1) and plasmid (GenBank: CP001362.1) sequences are available for strain 14028s.
Each vial contains approximately 0.5 mL of bacterial culture in 0.5X LB broth (supplemented with 1% Bacto-tryptone, 0.5% yeast extract and 1% sodium chloride) with 10% glycerol.
Salmonella enterica (S. enterica) subsp. enterica, strain 14028s ΔSTM14:2908 (serovar Typhimurium) was derived from strain 14028s (strain 14028s was originally known as strain 14028, however, a variant of the original strain with a rough colony morphology was designated 14028r and the original smooth strain was renamed 14028s). Strain 14028 is a descendent of strain CDC 6516-60 which was isolated from pools of hearts and livers of 4-week-old chickens.
The ΔSTM14:2908 mutant of strain 14028s was produced by creating a PCR product with STM14:2908 homologous sequences at the 5' and 3' ends of a linear fragment containing a kanamycin resistance cassette. S. enterica subsp. enterica, strain 14028s was transformed, and insertion of the kan cassette in place of STM14:2908 was confirmed by PCR. The final non-polar deletions were constructed by elimination of the kan cassette. The complete genome (GenBank: CP001363.1) and plasmid (GenBank: CP001362.1) sequences are available for strain 14028s.
Each vial contains approximately 0.5 mL of bacterial culture in 0.5X LB broth (supplemented with 1% Bacto-tryptone, 0.5% yeast extract and 1% sodium chloride) with 10% glycerol.
主要内容
此项目的每个订单数量限制为1.此商品每年可订购两次.在此限制上订单将在发货前发送到NIAID进行批准. Salmonella entenica(S. enterica)子公司. 肠溶,菌株14028sΔstm14:2908(血清虫毛螺旋藻)衍生出菌株14028s(菌株14028s最初称为菌株14028,然而,用粗糙菌落形态的原始菌株的变体被指定为14028r和原始的光滑菌株重命名为14028s).菌株14028是菌株CDC 6516-60的后代,其与4周龄鸡的心脏和肝脏池中分离出来. ΔStm14:2908突变体通过产生PCR产物产生14028s的菌株STM14:2908在含有卡那霉素抵抗盒的线性片段的5'和3'末端的同源序列. s. enterica 子公司.转化菌株14028s,菌株14028s,并通过PCR确认代替STM14:2908的盒的插入盒.通过消除 KaN 盒来构建最终的非极性缺失.完整的基因组(Genbank: cp001363.1 )和质粒(Genbank:CP001362.1 )序列可用于应变14028s. 每个小瓶含有约0.5毫升的细菌0.5X LB肉汤中的培养物(补充有1%的豆荚酮,0.5%酵母提取物和1%氯化钠),其中10%甘油.
厂牌介绍
BEI Resources 由美国国家过敏和传染病研究所 ( NIAID )成立,旨在为研究 A、B 和 C 类优先病原体、新兴传染病病原体、非病原微生物和其他相关微生物材料提供试剂、工具和信息到研究界。BEI Resources 获取、验证和生产科学家进行基础研究和开发改进的诊断测试、疫苗和疗法所需的试剂。通过将这些功能集中在 BEI Resources 中,可以监控科学界对这些材料的访问和使用,并确保试剂的质量控制。
除了为传染病界提供材料外,BEI Resources 还鼓励和支持研究人员和机构的材料存放。使用 BEI Resources存放材料对研究人员和研究社区有许多优势,包括安全存储、社区访问和分发;同时保护存款人的知识产权。只要有需要,BEI 资源库将作为研究人员的资源进行维护。您在 BEI Resources 的存款是一项有助于未来研究的长期投资。
BEI Resources 自 2003 年起由美国典型培养物保藏中心 (ATCC) 根据合同管理。2016 年 5 月, ATCC获得了一份为期七年的继续管理 BEI Resources的合同。合同范围已扩大到更全面的研究目录材料,包括由其他政府支持的研究项目存放的材料,将提供给生物防御和新兴传染病科学界。真菌、寄生虫、载体和其他相关材料已添加到现有的细菌、病毒和毒素试剂中,涵盖 NIAID A、B 和 C 类优先病原体和 NIAID 指定的新发传染病病原体和生物。
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