NR-36205Caviaporcellus,豚鼠表达克隆CD8α/pUB6A(克隆）

NR-36205??Cavia porcellus, Guinea Pig Expression Clone CD8α/pUB6A(Clones)|Cavia porcellus|Guinea Pig Expression Clone CD8α/pUB6A|-20°C or colder|RG RankAcknowledgment for publications should read "The following reagent was obtained through BEI Resources, NIAID, NIH: Guinea Pig Expression Clone CD8α/pUB6A, NR-36205."|Quantity limit per order for this item is 1. This item can be ordered twice a year. Orders over this limit will be sent to NIAID for approval before shipment.NR-36205 is a full-length expression clone containing the CD8α gene (GenBank: NM_001172876) from guinea pig (Cavia porcellus). The CD8α gene was cloned into vector pUB6A via BamHI and EcoRV insertion sites, transformed into MAX Efficiency^?Escherichia coli (E. coli) DH5α? competent cells (Invitrogen?), and extracted using a QIAGEN^? Plasmid Mega Kit.Note: Sequencing results for NR-36205 revealed that most regions of the vector match the depositor's information, including the ampicillin resistance gene, ubiquitin C promoter, polyadenylation signals, and the pMB1 and f1 origins of replication. However, the blasticidin resistance gene and associated EM-7 promoter normally encoded by pUB6A have been completely replaced with the hygromycin resistance gene under control of the SV40 promoter (presumably taken from the pcDNA 3.1 vector). The resulting size of the plasmid is 6.801 kilobases. The plasmid map and the complete plasmid sequence are provided on the Certificate of Analysis for NR-36205.Each vial of NR-36205 contains approximately 1 ?g of plasmid DNA in TE buffer.Note: The vial labels for NR-36205 incorrectly state that the plasmid DNA is provided as an E. coli glycerol stock. NR-36205 is purified plasmid DNA that was extracted from bacteria prior to vialing.