NR-36060_血吸虫属,用于在血吸虫中表达的载体pLNHX_cHS4_650,在大肠杆菌中重组(质粒/载体）

NR-36060??Schistosoma spp., Vector pLNHX_cHS4_650 for Expression in Schistosoma, Recombinant in Escherichia coli(Plasmid/Vectors)|Schistosoma spp.|Vector pLNHX_cHS4_650 for Expression in Schistosoma, Recombinant in Escherichia coli|-60°C or colder|P BrindleyAcknowledgment for publications should read "The following reagent was provided by the NIAID Schistosomiasis Resource Center for distribution through BEI Resources, NIAID, NIH: Vector pLNHX_cHS4_650 for Expression in Schistosoma, Recombinant in Escherichia coli, NR-36060."|Quantity limit for this item is 2 per year. Orders over this limit will be sent to NIAID for approval before shipment.Vector pLNHX_cHS4_650 was derived from pLNHXΔD70 (BEI Resources NR-36059) which was derived from the Clontech Laboratories vector pLNHX, a vector designed for retroviral gene delivery and expression. Once pLNHX is transfected into a packaging cell line, pLNHX can express a transcript containing the viral packaging signal, the neomycin selection marker and a target gene. To produce pLNHX_cHS4_650, pLNHX was digested with XhoI to remove the Drosophila heat shock protein 70 promoter resulting in pLNHXΔD70. pLNHXΔD70 was further modified by insertion of a 650 bp portion of the cHS4 insulator (including 250 bp of the core DNA sequence from the 5' end of the cHS4 fused with 400 bp from the 3' end of the cHS4) into the XbaI site of the U3 region of the 3'-LTR region to produce pLNHX_cHS4_650. The cHS4 element is a chromatin insulator which can block the interactions of the globin gene promoters and enhancers in cell lines and can protect expression cassettes in transformed cell lines and transgenic mammals from position effects. Shorter versions of the cHS4 insulator are known to retain the insulator activity of the full length element. Both vectors can be used for transduction of Schistosoma.Vector: pLNHX_cHS4_650 (GenBank: JN000001)Selection: Ampicillin (Prokaryotic) and Neomycin (Eukaryotic)Each vial contains approximately 1 mL of Escherichia coli DH5α, transformed with vector pLNHX_cHS4_650, in SOC media (Super Optimal broth with Catabolite repression) supplemented with 15% glycerol.