- 4 次围观
产品图片
货号/SKU
NR-15640
货号/规格
EA
库存与交货期
3-8周
人民币价格
14000
试剂海关审批
A/B级风险物质只能直接使用人购买并持有实验室有效资质,其它询客服确认
国外采购
支持/部分限制一年内购买数量
厂牌
BEI Resources(ATCC)
产品基础信息
生物安全等级建议分类:美国、2
产品描述信息
NR-15640??Mus musculus, Microglial Cell Line Derived from TRIF/MyD88 Double Knockout Mice(Cell Banks)|Mus musculus|Microglial Cell Line Derived from TRIF/MyD88 Double Knockout Mice|-100°C or colder, preferably in the vapor phase of a liquid nitrogen freezer|D GolenbockAcknowledgment for publications should read "The following reagent was obtained through BEI Resources, NIAID, NIH: Microglial Cell Line Derived from TRIF/MyD88 Double Knockout Mice, NR-15640."|Quantity limit per order for this item is 1. This item can be ordered twice a year. Orders over this limit will be sent to NIAID for approval before shipment.
The murine microglial cell line, NR-15640, was derived using brain tissue from TRIF (toll-interleukin-1 receptor domain-containing adaptor-inducing interferon-?)/MyD88 (myeloid differentiation primary response protein 88) double knockout mice. The microglial cells were immortalized by infection with the ecotropic transforming replication-deficient retrovirus J2 using techniques described in the literature. Characterization based on immunofluorescence, stimulation assays and flow cytometry demonstrated that the immortalized cell line retains its microglial-specific morphological, functional and surface expression properties.
Each vial contains approximately 1 mL of cell culture suspension frozen in cell growth medium (90%) and DMSO (10%) cryopreservative. Sufficient cells are provided to initiate at least one new culture.
The murine microglial cell line, NR-15640, was derived using brain tissue from TRIF (toll-interleukin-1 receptor domain-containing adaptor-inducing interferon-?)/MyD88 (myeloid differentiation primary response protein 88) double knockout mice. The microglial cells were immortalized by infection with the ecotropic transforming replication-deficient retrovirus J2 using techniques described in the literature. Characterization based on immunofluorescence, stimulation assays and flow cytometry demonstrated that the immortalized cell line retains its microglial-specific morphological, functional and surface expression properties.
Each vial contains approximately 1 mL of cell culture suspension frozen in cell growth medium (90%) and DMSO (10%) cryopreservative. Sufficient cells are provided to initiate at least one new culture.
主要内容
此项目的每个订单数量限制为1.此商品每年可订购两次.此限制的订单将在发货前发送到NIAID批准.
使用来自TRIF的脑组织(含Toll-Interleukin-1受体结构域的适配器诱导干扰素-β)/ myD88(骨髓分化初级反应蛋白88)双敲除小鼠,鼠髓细胞系NR-15640的NR-15640衍生出来衍生出来自TRIF的脑组织.使用文献中描述的技术感染生态转化转化复制缺陷型逆转录病毒J2,使微胶质细胞永生化.基于免疫荧光,刺激测定和流式细胞仪的表征证明了永生化的细胞系保留了其微胶质特异性形态学,功能性和表面表达特性.
每个小瓶含有约1ml细胞生长培养基(90%)和DMSO(10%)冷冻保存的细胞培养悬浮液.提供足够的细胞以引发至少一种新培养物.
厂牌介绍
BEI Resources 由美国国家过敏和传染病研究所 ( NIAID )成立,旨在为研究 A、B 和 C 类优先病原体、新兴传染病病原体、非病原微生物和其他相关微生物材料提供试剂、工具和信息到研究界。BEI Resources 获取、验证和生产科学家进行基础研究和开发改进的诊断测试、疫苗和疗法所需的试剂。通过将这些功能集中在 BEI Resources 中,可以监控科学界对这些材料的访问和使用,并确保试剂的质量控制。
除了为传染病界提供材料外,BEI Resources 还鼓励和支持研究人员和机构的材料存放。使用 BEI Resources存放材料对研究人员和研究社区有许多优势,包括安全存储、社区访问和分发;同时保护存款人的知识产权。只要有需要,BEI 资源库将作为研究人员的资源进行维护。您在 BEI Resources 的存款是一项有助于未来研究的长期投资。
BEI Resources 自 2003 年起由美国典型培养物保藏中心 (ATCC) 根据合同管理。2016 年 5 月, ATCC获得了一份为期七年的继续管理 BEI Resources的合同。合同范围已扩大到更全面的研究目录材料,包括由其他政府支持的研究项目存放的材料,将提供给生物防御和新兴传染病科学界。真菌、寄生虫、载体和其他相关材料已添加到现有的细菌、病毒和毒素试剂中,涵盖 NIAID A、B 和 C 类优先病原体和 NIAID 指定的新发传染病病原体和生物。
品牌标识
可能感兴趣的内容
2021-12-21
2024-01-21
2021-12-21
2022-04-01
2021-12-21
2020-06-09
2024-05-19
2020-08-04
2022-04-01
2022-04-01
2024-08-16
2022-04-01
2022-01-10
2021-12-21
2022-04-01
2022-04-01