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产品图片
货号/SKU
NR-15204
货号/规格
EA
库存与交货期
3-8周
人民币价格
14000
试剂海关审批
A/B级风险物质只能直接使用人购买并持有实验室有效资质，其它询客服确认
国外采购
支持/部分限制一年内购买数量
厂牌
BEI Resources(ATCC)
产品基础信息
生物安全等级建议分类：美国、1
产品描述信息
NR-15204?? Modified pCAGGS Vector Containing the SARS Coronavirus, Urbani Non-Structural Protein 3C Gene(Plasmid/Vectors)|SARS Coronavirus|Modified pCAGGS Vector Containing the SARS Coronavirus, Urbani Non-Structural Protein 3C Gene| -20°C or colder |P KuhnAcknowledgment for publications should read “The following reagent was obtained through BEI Resources, NIAID, NIH: Modified pCAGGS Vector Containing the SARS Coronavirus, Urbani Non-Structural Protein 3C Gene, NR-15204.”|Quantity limit per order for this item is 1. This item can be ordered twice a year. Orders over this limit will be sent to NIAID for approval before shipment.
The non-structural protein 3 (nsp3) gene from severe acute respiratory syndrome coronavirus (SARS-CoV), Urbani (GenBank: AY278741) was designed for expression of a C-terminal NSP3 fragment (residues 1319-1922; NSP3C) and cloned into the modified pCAGGS mammalian expression vector. pCAGGS was modified by adding a hemagglutinin (HA) tag as well as a tobacco etch virus (TEV) cleavable BirA biotinylation tag C-terminal to the insert coding sequence. NR-15204 contains the beta-lactamase gene, bla, to provide transformant selection through ampicillin resistance in Escherichia coli (E. coli). The complete plasmid sequence and map are provided on the BEI Resources webpage. The plasmid was produced in E. coli and extracted.
NSP3 is a multidomain protein located within the SARS-CoV ORF1ab polyprotein. The C-terminal fragment of NSP3 includes the ectodomain, Y1 and CoV-Y domains, although the exact function of this fragment is still under study. Together with NSP4 and NSP6, NSP3 induces the formation of double-membrane vesicles, which are critical structures required for viral replication.
Each vial contains plasmid DNA in TE buffer (10 mM Tris-HCl, 1 mM EDTA, pH 8.0). The vial should be centrifuged prior to opening. Note: The contents of the vial should be used to replicate the plasmid in E. coli prior to mammalian expression.
Additional information and tools are available at ViPR (Virus Pathogen Resource).
The non-structural protein 3 (nsp3) gene from severe acute respiratory syndrome coronavirus (SARS-CoV), Urbani (GenBank: AY278741) was designed for expression of a C-terminal NSP3 fragment (residues 1319-1922; NSP3C) and cloned into the modified pCAGGS mammalian expression vector. pCAGGS was modified by adding a hemagglutinin (HA) tag as well as a tobacco etch virus (TEV) cleavable BirA biotinylation tag C-terminal to the insert coding sequence. NR-15204 contains the beta-lactamase gene, bla, to provide transformant selection through ampicillin resistance in Escherichia coli (E. coli). The complete plasmid sequence and map are provided on the BEI Resources webpage. The plasmid was produced in E. coli and extracted.
NSP3 is a multidomain protein located within the SARS-CoV ORF1ab polyprotein. The C-terminal fragment of NSP3 includes the ectodomain, Y1 and CoV-Y domains, although the exact function of this fragment is still under study. Together with NSP4 and NSP6, NSP3 induces the formation of double-membrane vesicles, which are critical structures required for viral replication.
Each vial contains plasmid DNA in TE buffer (10 mM Tris-HCl, 1 mM EDTA, pH 8.0). The vial should be centrifuged prior to opening. Note: The contents of the vial should be used to replicate the plasmid in E. coli prior to mammalian expression.
Additional information and tools are available at ViPR (Virus Pathogen Resource).
主要内容
此项目的每个订单数量限制为1.此商品每年可订购两次.此限制的订单将在发货前发送到NIAID批准.
来自严重急性呼吸综合征Coronavirus（SARS-COV）的非结构蛋白3（NSP3）基因，Urbani（Genbank： AY278741 ）设计用于表达C末端NSP3片段（残留物1319-1922; NSP3C）并将其克隆到修改后的 pcaggs 哺乳动物表达载体.通过添加血凝素（HA）标签以及烟草蚀刻病毒（TEV）可切割的Bira Biotinylation标签C末端通过添加Hemagglutinin（HA）标签来修饰PCAGGs. NR-15204含有β-内酰胺酶基因， BLA ，以通过氨苄青霉素抗性在大肠杆菌（大肠杆菌）中提供转化体选择.在北部资源网页上提供完整的质粒序列和地图.在 e中制备质粒. Coli 并提取.
NSP3是位于SARS-COV ORF1ab Polyprotein内的多麦粉蛋白. NSP3的C末端片段包括突突，Y1和COV-Y结构域，尽管该片段的确切功能仍在研究中.与NSP4和NSP6一起，NSP3诱导双膜的形成
囊泡，这是病毒复制所需的关键结构.
每个小瓶在Te缓冲液中含有质粒DNA（10mM Tris-HCl，1mM EDTA，pH8.0）.小瓶应该是
在打开之前离心. 注意：应使用小瓶的内容来复制
在 e中的质粒. Coli 在哺乳动物表达之前.
vipr （病毒病原体资源）提供了附加信息和工具.
厂牌介绍
BEI Resources 由美国国家过敏和传染病研究所 ( NIAID )成立，旨在为研究 A、B 和 C 类优先病原体、新兴传染病病原体、非病原微生物和其他相关微生物材料提供试剂、工具和信息到研究界。BEI Resources 获取、验证和生产科学家进行基础研究和开发改进的诊断测试、疫苗和疗法所需的试剂。通过将这些功能集中在 BEI Resources 中，可以监控科学界对这些材料的访问和使用，并确保试剂的质量控制。
除了为传染病界提供材料外，BEI Resources 还鼓励和支持研究人员和机构的材料存放。使用 BEI Resources存放材料对研究人员和研究社区有许多优势，包括安全存储、社区访问和分发；同时保护存款人的知识产权。只要有需要，BEI 资源库将作为研究人员的资源进行维护。您在 BEI Resources 的存款是一项有助于未来研究的长期投资。
BEI Resources 自 2003 年起由美国典型培养物保藏中心 (ATCC) 根据合同管理。2016 年 5 月， ATCC获得了一份为期七年的继续管理 BEI Resources的合同。合同范围已扩大到更全面的研究目录材料，包括由其他政府支持的研究项目存放的材料，将提供给生物防御和新兴传染病科学界。真菌、寄生虫、载体和其他相关材料已添加到现有的细菌、病毒和毒素试剂中，涵盖 NIAID A、B 和 C 类优先病原体和 NIAID 指定的新发传染病病原体和生物。
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