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产品图片
货号/SKU
NR-12208
货号/规格
EA
库存与交货期
3-8周
人民币价格
14000
试剂海关审批
A/B级风险物质只能直接使用人购买并持有实验室有效资质,其它询客服确认
国外采购
支持/部分限制一年内购买数量
厂牌
BEI Resources(ATCC)
产品基础信息
生物安全等级建议分类:美国、1
产品描述信息
NR-12208?? Monoclonal Anti-Sudan Ebolavirus Envelope Glycoprotein, Clone 6D11 (produced in vitro)(Monoclonal Antibodies)|Sudan ebolavirus|Monoclonal Anti-Sudan Ebolavirus Envelope Glycoprotein, Clone 6D11 (produced in vitro)|-20°C or colder|B HaynesAcknowledgment for publications should read "The following reagent was obtained through BEI Resources, NIAID, NIH: Monoclonal Anti-Sudan Ebolavirus Envelope Glycoprotein, Clone 6D11 (produced in vitro), NR-12208."|Quantity limit per order for this item is 1. This item can be ordered twice a year. Orders over this limit will be sent to NIAID for approval.
Antibody Class: IgG1?
Mouse monoclonal antibody prepared against the envelope glycoprotein (GP) of Sudan ebolavirus (EBOV) was purified from clone 6D11 hybridoma supernatant by protein G affinity chromatography. The B cell hybridoma was generated by the fusion of P3X63-Ag8 BALB/c mouse myeloma cells with splenocytes from female BALB/c mice that had been immunized intramuscularly with VRC6204 plasmid and boosted with purified recombinant GP of the Sudan EBOV Gulu strain. VRC6204 consists of a synthetic human codon-optimized gene expressing the transmembrane-deleted GP of the Sudan EBOV Gulu strain.
Note: The P3X63-Ag8 myeloma cell line secretes the MOPC21 myeloma protein, a mouse IgG1? antibody of unknown specificity. Thus, NR-12208 may contain both MOPC21 protein and EBOV GP-specific antibody of the IgG1? isotype, as well as inactive hybrid immunoglobulin molecules.
Each vial of NR-12208 contains approximately 100 ?L of purified monoclonal antibody in PBS.
NR-12208 is reported to recognize Sudan EBOV GP in western blot assays and not to cross-react with other known EBOV species.
Antibody Class: IgG1?
Mouse monoclonal antibody prepared against the envelope glycoprotein (GP) of Sudan ebolavirus (EBOV) was purified from clone 6D11 hybridoma supernatant by protein G affinity chromatography. The B cell hybridoma was generated by the fusion of P3X63-Ag8 BALB/c mouse myeloma cells with splenocytes from female BALB/c mice that had been immunized intramuscularly with VRC6204 plasmid and boosted with purified recombinant GP of the Sudan EBOV Gulu strain. VRC6204 consists of a synthetic human codon-optimized gene expressing the transmembrane-deleted GP of the Sudan EBOV Gulu strain.
Note: The P3X63-Ag8 myeloma cell line secretes the MOPC21 myeloma protein, a mouse IgG1? antibody of unknown specificity. Thus, NR-12208 may contain both MOPC21 protein and EBOV GP-specific antibody of the IgG1? isotype, as well as inactive hybrid immunoglobulin molecules.
Each vial of NR-12208 contains approximately 100 ?L of purified monoclonal antibody in PBS.
NR-12208 is reported to recognize Sudan EBOV GP in western blot assays and not to cross-react with other known EBOV species.
主要内容
此项目的每个订单数量限制为1.此商品每年可订购两次.通过此限制的订单将被发送到NIAID进行批准. 抗体类:IgG1?对苏丹·糖蛋白(EBOV)的封套糖蛋白(GP)制备的小鼠单克隆抗体纯化克隆6d11杂交瘤上清液通过蛋白质g亲和层析. B细胞杂交瘤由P3X63-AG8 BALB / C小鼠骨髓瘤细胞的熔化产生,含有VRC6204质粒的肌肉BALB / C小鼠的脾细胞,并用苏丹EBOV GULU菌株的纯化重组GP升压. VRC6204包括一种表达苏丹EBOV Gulu菌株的跨膜已删除GP的合成人码子优化基因. 注意:P3X63-AG8骨髓瘤细胞系分泌MOPC21骨髓瘤蛋白,小鼠IgG1?特异性未知的抗体.因此,NR-12208可以含有IgG1的MOPC21蛋白和EBOV GP特异性抗体?同种型,以及无活性的混合免疫球蛋白分子. 每个小瓶中的每个小瓶在PBS中含有大约100μl纯化的单克隆抗体. Western印迹测定和与其他已知的EBOV物种交叉反应.
厂牌介绍
BEI Resources 由美国国家过敏和传染病研究所 ( NIAID )成立,旨在为研究 A、B 和 C 类优先病原体、新兴传染病病原体、非病原微生物和其他相关微生物材料提供试剂、工具和信息到研究界。BEI Resources 获取、验证和生产科学家进行基础研究和开发改进的诊断测试、疫苗和疗法所需的试剂。通过将这些功能集中在 BEI Resources 中,可以监控科学界对这些材料的访问和使用,并确保试剂的质量控制。
除了为传染病界提供材料外,BEI Resources 还鼓励和支持研究人员和机构的材料存放。使用 BEI Resources存放材料对研究人员和研究社区有许多优势,包括安全存储、社区访问和分发;同时保护存款人的知识产权。只要有需要,BEI 资源库将作为研究人员的资源进行维护。您在 BEI Resources 的存款是一项有助于未来研究的长期投资。
BEI Resources 自 2003 年起由美国典型培养物保藏中心 (ATCC) 根据合同管理。2016 年 5 月, ATCC获得了一份为期七年的继续管理 BEI Resources的合同。合同范围已扩大到更全面的研究目录材料,包括由其他政府支持的研究项目存放的材料,将提供给生物防御和新兴传染病科学界。真菌、寄生虫、载体和其他相关材料已添加到现有的细菌、病毒和毒素试剂中,涵盖 NIAID A、B 和 C 类优先病原体和 NIAID 指定的新发传染病病原体和生物。
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