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产品图片
货号/SKU
NR-12186
货号/规格
EA
库存与交货期
3-8周
人民币价格
14000
试剂海关审批
A/B级风险物质只能直接使用人购买并持有实验室有效资质,其它询客服确认
国外采购
支持/部分限制一年内购买数量
厂牌
BEI Resources(ATCC)
产品基础信息
生物安全等级建议分类:美国、1
产品描述信息
NR-12186?? Monoclonal Anti-Bacillus anthracis Protective Antigen, Clone PA-27H11 (produced in vitro)(Monoclonal Antibodies)|Bacillus anthracis|Monoclonal Anti-Bacillus anthracis Protective Antigen, Clone PA-27H11 (produced in vitro)|-20°C or colder|B HaynesAcknowledgment for publications should read "The following reagent was obtained through BEI Resources, NIAID, NIH: Monoclonal Anti-Bacillus anthracis Protective Antigen, Clone PA-27H11 (produced in vitro), NR-12186."|Quantity limit per order for this item is 1. This item can be ordered twice a year. Orders over this limit will be sent to NIAID for approval.
Antibody Class: IgG1κ and IgG2bκ
Monoclonal antibody prepared against recombinant protective antigen (rPA) from Bacillus anthracis (B. anthracis) was purified from hybridoma clone PA-27H11 supernatant by protein G affinity chromatography. The B cell hybridoma was generated by the fusion of P3X63-Ag8 BALB/c mouse myeloma cells with splenocytes from female C57BL/6 x BALB/c F1 mice immunized intranasally with purified rPA.
Note: The P3X63-Ag8 myeloma cell line secretes the MOPC21 myeloma protein, a mouse IgG1κ antibody of unknown specificity. Thus, NR-12186 contains both MOPC21 protein and B. anthracis PA-specific antibody of the IgG2bκ isotype, as well as inactive hybrid immunoglobulin molecules.
Each vial of NR-12186 contains approximately 100 ?L of purified monoclonal antibody in PBS.
NR-12186 is released without confirmation of functional activity. Clone PA-27H11 antibody is reported to bind B. anthracis PA in ELISA and surface plasmon resonance assays. The antibody did not neutralize anthrax lethal toxin in cell-based assays.
Antibody Class: IgG1κ and IgG2bκ
Monoclonal antibody prepared against recombinant protective antigen (rPA) from Bacillus anthracis (B. anthracis) was purified from hybridoma clone PA-27H11 supernatant by protein G affinity chromatography. The B cell hybridoma was generated by the fusion of P3X63-Ag8 BALB/c mouse myeloma cells with splenocytes from female C57BL/6 x BALB/c F1 mice immunized intranasally with purified rPA.
Note: The P3X63-Ag8 myeloma cell line secretes the MOPC21 myeloma protein, a mouse IgG1κ antibody of unknown specificity. Thus, NR-12186 contains both MOPC21 protein and B. anthracis PA-specific antibody of the IgG2bκ isotype, as well as inactive hybrid immunoglobulin molecules.
Each vial of NR-12186 contains approximately 100 ?L of purified monoclonal antibody in PBS.
NR-12186 is released without confirmation of functional activity. Clone PA-27H11 antibody is reported to bind B. anthracis PA in ELISA and surface plasmon resonance assays. The antibody did not neutralize anthrax lethal toxin in cell-based assays.
主要内容
此项目的每个订单数量限制为1.此项目每年可订购两次.通过此限制的订单将被发送到NIAID进行批准. 抗体类:IgG1κ和IgG2bκ 对重组保护抗原制备的单克隆抗体(RPA)从芽孢杆菌(B.炭疽)通过蛋白G亲和色谱法从杂交瘤克隆PA-27H11上清液中纯化.通过P3X63-AG8 BALB / C小鼠骨髓瘤细胞的融合产生B细胞杂交瘤,含有纯化的RPA免疫的雌性C57BL / 6 x BALB / C F1小鼠的脾细胞. 注意:P3X63-AG8骨髓瘤细胞系分泌MOPC21骨髓瘤蛋白,一种未知特异性的小鼠IgG1κ抗体.因此,NR-12186含有MOPC21蛋白和 B. IgG2Bκ同种型的炭疽感和Pa特异性抗体,以及无活性的杂交免疫球蛋白分子. 每个小瓶NR-12186在PBS中含有约100μl纯化的单克隆抗体. NR-12186未经确认功能活性释放.据报道克隆PA-27H11抗体结合 b.炭疽生长在ELISA和表面等离子体共振测定中.抗体在基于细胞的测定中没有中和炭疽致命毒素.
厂牌介绍
BEI Resources 由美国国家过敏和传染病研究所 ( NIAID )成立,旨在为研究 A、B 和 C 类优先病原体、新兴传染病病原体、非病原微生物和其他相关微生物材料提供试剂、工具和信息到研究界。BEI Resources 获取、验证和生产科学家进行基础研究和开发改进的诊断测试、疫苗和疗法所需的试剂。通过将这些功能集中在 BEI Resources 中,可以监控科学界对这些材料的访问和使用,并确保试剂的质量控制。
除了为传染病界提供材料外,BEI Resources 还鼓励和支持研究人员和机构的材料存放。使用 BEI Resources存放材料对研究人员和研究社区有许多优势,包括安全存储、社区访问和分发;同时保护存款人的知识产权。只要有需要,BEI 资源库将作为研究人员的资源进行维护。您在 BEI Resources 的存款是一项有助于未来研究的长期投资。
BEI Resources 自 2003 年起由美国典型培养物保藏中心 (ATCC) 根据合同管理。2016 年 5 月, ATCC获得了一份为期七年的继续管理 BEI Resources的合同。合同范围已扩大到更全面的研究目录材料,包括由其他政府支持的研究项目存放的材料,将提供给生物防御和新兴传染病科学界。真菌、寄生虫、载体和其他相关材料已添加到现有的细菌、病毒和毒素试剂中,涵盖 NIAID A、B 和 C 类优先病原体和 NIAID 指定的新发传染病病原体和生物。
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