MRA-912质粒pHTH,用于恶性疟原虫的转染(质粒/载体）

MRA-912?? Plasmid pHTH, for Transfection in Plasmodium falciparum(Plasmid/Vectors)|Plasmodium falciparum|Plasmid pHTH, for Transfection in Plasmodium falciparum|-80°C or colder|JH AdamsAcknowledgment for publications should read "The following reagent was obtained through BEI Resources, NIAID, NIH: Plasmid pHTH, for Transfection in Plasmodium falciparum, MRA-912, contributed by John Adams."|Quantity limit per order for this item is 1. This item can be ordered twice a year. Orders over this limit will be sent to NIAID for approval before shipment.MRA-912 is a cotransfection plasmid, pHTH, featuring a piggyBac transposase. Plasmid pHTH contains a 5'HSP86, 3'HSP86, CDS-piggyBac Transposase expression cassette, designed for piggyBac-mediated transformation of Plasmodium falciparum parasites. The resulting size of the plasmid is 8.792 kilobases.Each vial of MRA-912 contains approximately 500 ng of plasmid DNA in TE buffer at a concentration of 20 ng/?L.Cary, L. C., et al. "Transposon Mutagenesis of Baculoviruses: Analysis of Trichoplusia ni Transposon IFP2 Insertions within the FP-Locus of Nuclear Polyhedrosis Viruses." Virology 172 (1989): 156-169. PubMed: 2549707.Balu, B.,et al. "High-Efficiency Transformation of Plasmodium falciparum by the Lepidopteran Transposable Element piggyBac." Proc. Natl. Acad. Sci. USA 102 (2005): 16391-16396. PubMed: 16260745.Fraser, M. J., et al. "Assay for Movement of Lepidopteran Transposon IFP2 in Insect Cells Using a Baculovirus Genome as a Target DNA." Virology 211 (1995): 397-407. PubMed: 7645244.